托尔斯泰《艺术论》在中国

托尔斯泰的《艺术论》作为世界一流小说家比较系统的艺术理论著作,以呼吁艺术同美脱钩,专事情感交流,且以宗教性和世界性为伟大作品的标准,在19世纪美学中独树一帜。《艺术论》在中国的接受,以1921年、1958年和2005年的三个中译本为界,可分别以人生的艺术、宗教意识批判,以及意识形态的重新认可为其标识。可以说,《艺术论》在中国的接受史,大体也反映出中国一百年以来文艺理论的基本走向。     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

可卡因对小鼠离体腹腔巨噬细胞功能的影响

目的研究可卡因对体外培养小鼠腹腔巨噬细胞功能的影响。方法无菌分离小鼠腹腔巨噬细胞,制备单细胞悬液,分别用不同浓度的可卡因作用于正常的和LPS活化的腹腔巨噬细胞。MTT法检测其增殖反应;ELISA法检测巨噬细胞培养上清中TNF-α的分泌水平;生物活性检测法检测IL-1的活性。结果可卡因在终浓度10、20和100μtg／mL时能明显抑制LPS刺激的腹腔巨噬细胞增殖反应,减少TNF-α、IL．1的释放,与对照组相比较有显著性差异（P〈0．01）。结论可卡因对体外培养腹腔巨噬细胞功能有明显抑制作用。     

利用RASSF1A位点检测母体血浆中胎儿SNP分型

目的探讨利用母体血浆中高甲基化RASSF1A位点进行胎儿SNP分型的应用价值。方法随机收集10个未孕健康妇女和45例不同孕期（早期5例、中期20例、晚期20例）孕妇的血样本及相应胎儿组织（绒毛组织、羊水、胎盘组织）;利用甲基化敏感限制性内切酶BstUI酶切后进行PCR,产物进行血细胞、血浆和胎儿组织（绒毛或胎盘）DNA RASSF1A序列的甲基化模式检测,并采用直接测序法对SNP rs4688725位点进行分型。结果经BstUI酶消化,RASSF1A序列在母体血细胞中均未检出,而在绒毛或胎盘组织中均能检出;在45名孕妇血浆中,RASSF1A序列均能被检出,且序列内的SNP分型与相应胎儿组织一致;在10名非孕妇女血浆中均未检出RASSF1A序列。结论母体和胎儿DNA中RASSF1A基因启动子区域的甲基化模式存在差异,可用于对母体血浆中的游离胎儿DNA进行SNP分型。     

Determination of Acetamiprid and IM‐1‐2 in PostMortem Human Blood,Liver, Stomach Contents by HPLC‐DAD

An HPLC‐DAD method was developed to detect and quantify a neonicotinoid insecticide acetamiprid (ATP) and its metabolite IM‐1‐2 in autopsy samples of a fatal intoxication case. The postmortem blood and tissue distribution of ATP and IM‐1‐2 was determined for the first time. The method showed acceptable precisions and recoveries with relative standard deviations of <10% for ATP level and 1.38 % for IM‐1‐2. The detection and quantification limits for ATP were 0.015 μg/mL and 0.030 μg/mL for blood and were 0.035 μg/g and 0.050 μg/g for liver samples, respectively. The mean contents of ATP were 0.79 μg/g in the liver, 47.35 μg/g in the stomach contents and 2.7 μg/mL in the blood. IM‐1‐2 content was 17.0 μg/g in the stomach contents. ATP and IM‐1‐2 were not detected in the urine. The presence of ATP and IM‐1‐2 in the samples was confirmed by GC‐MS. The method can be exploited in future forensic casework.     

双手第1骨间背侧肌代谢物的氢质子磁共振波谱

目的 探讨氢质子磁共振波谱（1H-magnetic resonance spectroscopy,1H-MRS）检测技术在尺神经损伤预后评估的应用前景.方法 对12例健康青年志愿者、1例尺神经完全损伤者双手第1骨间背侧肌的代谢物进行1H-MRS检测,对采集到的数据进行统计分析. 结果 健康女性成年人第1骨间背侧肌的细胞外脂质（extra-myocellular lipids,EMCL）峰下面积高于男性（P＜0.05）,胆碱、肌酸、细胞内脂质（intra-myocellular lipids,IMCL）在两性间差异均无统计学意义（P＞0.05）,双手间各峰下面积差异均无统计学意义（P＞0.05）.尺神经损伤者患侧EMCL峰较健侧偏高,且FDI面积缩小.结论 1H-MRS对肌肉代谢物的无创性以及定量检测对周围神经损伤的预后评估具有一定价值.     

胡锦涛“七一”讲话民族思想研究

胡锦涛"七一"讲话高度重视民族问题,表达了重要的民族思想,包括:一、坚持和完善民族区域自治制度。二、加强民族团结,维护国家统一。三、为中华民族伟大复兴而努力。中华民族伟大复兴内涵丰富。中华民族伟大复兴意义重大。     

State transformation goes nuclear: Chinese National Nuclear Companies’ expansion into Europe

Abstract

Most of the literature on state transformation focuses on China’s relations with African, Asian and Latin American countries and the National Oil Companies’ overseas expansion to show that China has become fragmented, decentralised and internationalised. This article contributes novel findings by focusing on China’s relations with Europe and the actions of China’s National Nuclear Companies (NNCs). It shows that NNCs, which have become relatively autonomous actors, often pursue their agendas of expansion into Europe without much coordination with, or even in contradiction to, other ministries’ agendas and interests, especially the Ministry of Foreign Affairs. Instead of being orchestrated by the central government, their expansion reflects considerable disorganisation and sometimes undermines China’s official strategy. The article demonstrates this through case studies of NNCs’ involvement in the UK and Romania.     

砂拉越华族族群母语教育与文化传承的维护:以古晋中华第一中学之兴办为个案研究

砂拉越位于婆罗洲,原为文莱属地,1841年白人拉惹政权建立后,大批华人在其招垦下移入砂拉越。在华文教育萌芽的初期,白人拉惹对其取自由放任的态度,直至20世纪20年代,因华人社会中的政治运动及海外华侨民族主义在各个华文学校的发展,才颁布学校注册法令,开始干涉砂拉越地区的华文教育。二战后,白人拉惹将砂拉越让渡给英国,新成立的殖民地政府急于统合境内各族群对砂拉越的认同,遂推动以英语为教学媒介语的国家教育制度,迫使砂拉越华文中等学校在改制成以英语授课的学校或独立中学之间做出选择。为保存族群母语教育,为华族子弟接受母语教育提供机会,在古晋地区创立的中华第一中学,决定成为一间华文独立中学。这间华文独立中学至今仍有千名以上学生,其校园中充满华族文化气息。本文对古晋中华第一中学的创立、发展、经营作一个案研究,期盼对吾人进一步了解战后砂拉越华族社会族群语言的维护及文化的传承有所助益。     

论群体性事件处置中说服的艺术

群体性事件基本上属于人民内部矛盾,因此,公安机关在处置过程中,应最大限度地运用解决人民内‘部矛盾的方法和手段解决问题。在群体性事件处置中,说服的条件是事件态势处于相对控制之下：说服的原则主要包括平等性原则、针对性原则、可信性原则和感染性原则;说服的过程主要包括明确目标、分析对象、实施劝说、改变行为;说服方法主要包括标签效应法、“自己人”效应法、亲情效应法和威信效应法等。