Bettelverordnung Fürstenfeld

Der zust?ndige Landesgesetzgeber hat bereits seiner Auffassung nach unerwünschte Formen der Bettelei (aggressives oder Betteln durch Minderj?hrige) verboten. Ein weitergehendes (absolutes) Bettelverbot mittels ortspolizeilicher V der Gemeinde ist mangels Vorliegens eines ?rtlichen Missstandes verfassungswidrig.     

O型口蹄疫病毒VP1基因和牛集落刺激因子基因的融合表达

将O型口蹄疫病毒VP1基因和牛集落刺激因子(GMCSF)成熟肽基因共同克隆到原核表达载体pGEX6P1中,转化RS21后经IPTG诱导,实现了重组融合蛋白BoGMCSF/VP1在大肠埃希氏菌RS21中的高效表达,表达产物经SDSPAGE和Westernblotting分析,重组的蛋白质分子质量约为66ku,与预期大小相符。表达蛋白占菌体总蛋白的30%,表达产物以包涵体形式存在;包涵体提取物经变性复性,用透析方法提纯,得到高纯度的表达蛋白。     

论文艺接受中的民俗心理

民俗心理是支配文艺接受主体的潜在的巨大合力,且渗透在文艺接受过程的每一个环节。接受主体的民俗心理对审美客体有筛选和吸附、同化功能,即主体对客体的认同或排斥或吸收。民俗心理结构是一个开放性的结构,它不断发展、扩充自己的内涵,因而生机勃勃,具有强盛的生命向力。尊重民俗心理是创作精典式艺术作品的基础。     

A family with unusual Kell genotypes

A family in which kKp^a, kKp^b, KKp^b and K^o are segregating confirms the depressing effect of Kp^a on the products of other Kell genes in cis. The significance of such unusual Kell genotypes in forensic testing is discussed.     

Population data on the forensic genetic markers: phosphoglucomutase-1, esterase D, erythrocyte acid phosphatase and glyoxylase I

Blood specimens from white and black sample populations from Baltimore, Maryland, were analyzed for the four most forensically important, polymorphic red cell enzyme systems-phosphoglucomutase-1, esterase D, erythrocyte acid phosphatase and glyoxalase I. The distributions of the phenotypes for each marker in each racial group were in Hardy-Weinberg equilibrium. The population data were similar to previously reported data for Whites and Blacks from different geographical locations within the United States.     

鸡传染性支气管炎病毒SY毒株生物学特性及其S1基因分子遗传变异分析

通过生物学特性研究 ,验证了分离自沈阳地区的SY毒株确实为 1株鸡传染性支气管炎病毒。病毒中和试验表明 ,SY血清型不同于参考毒株T、H52 、M41及国内其他流行株如HD、XB、DB等 ;S1基因序列分析表明 ,SY核苷酸序列及推导的氨基酸序列与参考毒株Beau、M41、H12 0 、N1 62、Gray、6/ 82和Ark99等毒株的同源率都低于 80 % ,进化关系与各参考毒株也相距甚远 ,糖基化位点出现 3个新位点 ,氨基酸疏水性区域也存在差异。     

Cerebral massive hemorrhage in congophilic angiopathy and its medicolegal significance

The principles of a special type of senile cerebromeningeal angiopathy, these days usually named “congophilic angiopathy”, are demonstrated on the basis of eight cases, complicated by spontaneous massive intracranial hemorrhages in seven and late traumatic hemorrhage in one of them. The occurrence of massive hemorrhages associated with congophilic angiopathy has until now only rarely been reported. The medicolegal significance of this vascular disease is emphasized by the eighth, traumatic, case, which became forensically relevant; the late massive hemorrhage had to be regarded as significantly favoured by amyloid angionecrosis, which is the main feature of congophilic angiopathy.     

外伤性癫痫患者癫痫灶中GABABR1和GluR1的表达

目的探讨外伤性癫痫患者颞叶癫痫灶组织γ-氨基丁酸B受体亚单位（gamma-aminobutyric acid B receptorsubunit,GABABR1）和谷氨酸受体亚单位（glutamic acid receptor subunit,GluR1）的表达变化,以期为阐释外伤性癫痫发生机制和法医学鉴定提供依据。方法收集15例外伤性癫痫患者脑皮质（外伤性癫痫组）1、5例非外性癫痫患者脑皮质（非外伤性癫痫组）、15例交通事故死亡者脑皮质（交通事故死亡对照组）,运用荧光免疫组化技术和RT-PCR技术观察GABABR1和GluR1的表达水平。结果外伤性癫痫组GABAB R1表达水平明显低于对照组,但高于非外伤性癫痫组;外伤性癫痫组GluR1表达水平明显高于对照组,但低于非外伤性癫痫组。结论 GABABR1和GluR1的表达水平的变化在外伤性癫痫病的发病过程中起着一定的作用,可以作为区分外伤性癫痫与非外伤性癫痫的病变化指标之一。     

实时定量PCR检测大鼠脑挫伤后HIF-1α mRNA的表达

目的研究大鼠脑挫伤后挫伤部位脑组织HIF-1α mRNA的表达随损伤时间变化的规律及其在脑损伤时间推断中的应用。方法以Feeney法建立大鼠闭合性脑挫伤模型,应用实时荧光定量PCR技术检测脑挫伤部位HIF-1α mRNA的表达,采用2-△△Ct法比较实验组与对照组脑组织HIF-1α mRNA表达的倍数关系。结果与对照组相比,HIF-1α mRNA的表达在伤后1h即达到高峰,随后下降,伤后24h出现一次较小高峰,3～14d逐渐恢复至对照组水平。结论脑挫伤后HIF-1α mRNA的表达随损伤时间的变化呈现出一定规律性,可作为推断早期脑挫伤经过时间的生物学指标之一。     

Data protection: The future of privacy

The Art. 29 Working Party (hereinafter “Art. 29 WP”) is an influential body comprised of representatives from the Member State Data Protection Authorities² established under the Data Protection Directive 95/46/EC, has recently issued an opinion with the Working Party on Police and Justice. This is quite significant, since the opinion sets out some of the issues that will need to be addressed in the lead up to the revision of the Data Protection Directive 95/46/EC.³ This comes at a time, when there have been discussions on the current application of the European Data Protection Directive to the internet,⁴ (such as social networking) and the recent European Commission’s consultation on the legal framework for the fundamental right to protection of personal data. Not least, there have been a number of cases brought before the European Court of Justice dealing with the partial implementation of the Data Protection Directive 95/46/EC.⁵The aim of this paper is to consider in detail the issues set out by the Art. 29 WP and the likely challenges in revising the Data Protection Directive 95/46/EC.