大鼠液压冲击脑损伤TGF-β1及其受体TβR I的表达

目的观察脑损伤后TGF-β1及其受体TβRI蛋白表达及其时序性变化,探讨脑损伤的分子机制及法医学脑损伤时间推断。方法用免疫组织化学方法观察大鼠液压冲击脑损伤后TGF-β1及其受体TβRI蛋白在伤后不同时间(30 min、1 h、3 h、6 h、12 h、1 d、3 d、7 d)的表达情况,以正常大鼠及手术大鼠作为对照。结果 (1)正常及手术对照组大鼠脑组织内有低水平的TGF-β1及TβRI表达;(2)脑损伤后1～3d,大脑皮质和脑干TGF-β1/TβRI蛋白表达逐渐增加,7 d时仍维持在高表达水平;(3)海马冲击后12 h～1 d逐渐增加,3 d时仍处于高表达水平,7 d时已开始下降。结论脑损伤可诱导TGF-β1/TβRI基因在脑内表达,其时序性改变可望用于法医学脑损伤时间推断。     

Data Protection: An Opening Door?

Swedish public law has incorporated a general rule of public access to documents, and to information in the wider sense, since the Freedom of the Press Act of 1766. On the Community level, the relationship between Regulation (EC) 45/2001 on data protection and Regulation (EC) 1049/2001 on public access to documents exemplifies the tension between the public's interest in scrutinising the administration carried on in its name, and the integrity of the individual. However, a similar tension exists between Sweden's Personal Data Act of 1998, implementing Directive 95/46, and the Freedom of the Press Act. A misuse model of data protection law, by seeking to concentrate on serious rather than technical infringements of privacy, might serve to ease the tension. The views expressed in this article are solely those of the author and are not attributable to any Community institution. This revised version was published online in August 2006 with corrections to the Cover Date.     

气候变化的国际法发展:从温室效应理论到《联合国气候变化框架公约》

本文考察了温室效应理论对缔结《联合国气候变化框架公约》的决定性影响 ,并对《联合国气候变化框架公约》的基本法律原则 (规则 )及其对国际环境立法的贡献进行了述评。     

警察出庭作证:透析与前瞻

警察作为证人出庭作证在国外十分普遍,然而我国一直将警察排除在证人之外,警察出庭作证少之又少。我国警察应当出庭作证,但应限制在特定范围之内。在目前情况下,警察出庭作证还存在较大障碍,这恰恰是我国刑事司法改革之所在。     

Reviews

Books reviewed:
Christine Gray, International Law and the Use of Force
Phoebe Okowa, State Responsibility for Transboundary Air Pollution in International Law
Brian Leiter, Objectivity in Law and Morals
Robert Wintemute and Mads Andenas (eds.), Legal Recognition of Same–Sex Partnerships: A Study of National, European and International Law
Gillian Douglas, An Introduction to Family Law
Sandra Fredman, Discrimination and Human Rights: The Case of Racism
John Monahan et al, Rethinking Risk Assessment: The MacArthur Study of Mental Disorder and Violence
Jenny Fleming and Ian Holland (eds.), Motivating Ministers to Morality     

第三人利益合同的变更和解除

建立第三人利益合同制度是完善我国合同法的迫切需要.其中一个重要的问题就是当事人是否可以像在两人合同中一样协议变更或解除第三人利益合同.在没有第三人同意的情况下,当事人行使变更权和解除权虽不应当完全否认,但是必须受到限制.具体标准取决于建立第三人利益合同制度的宗旨和该合同的特征.作为一般法规范的第三人利益合同的变更和解除制度将与我国现行合同法律法规共存,形成完整的体系.并在实践中按照特别法优于一般法的原则予以适用.     

冠状动脉内弹力膜缺损与冠心病猝死的关系

目的探讨冠状动脉内弹力膜(IEL)病变与冠心病猝死(SCD)的关系。方法从本单位近5年尸检案例中挑选60例病例和心脏标本,分为SCD组、对照组A和B组。每例分别在冠状动脉左前降支近段取材,常规制片,HE和双重组合特殊染色,显微镜下观察冠状动脉IEL,并用图像分析技术对特殊染色的IEL进行形态定量分析。结果60例中,SCD组(男性24例,女性6例,年龄30~83岁)的冠状动脉IEL缺失明显(缺失率=41.64%),未缺失的IEL失去原有弯曲度而变平直;对照组A(冠心病非猝死者,男性13例、女性2例,年龄41~80岁)的冠状动脉IEL缺失程度较SCD组稍轻(缺失率=30.53%);对照组B(无冠心病的其他死者,男性8例,女性7例,年龄17~54岁)的冠状动脉IEL仅有轻度缺失(缺失率=15.67%),多保持原有波浪状结构。统计学分析显示,SCD组与对照组、SCD组与对照组B、对照组A与对照组B之间IEL缺损率的差异均具有极显著性意义;SCD组与对照组A在IEL缺损率上的差异有显著性意义。结论SCD与其冠状动脉IEL缺损率的大小密切相关。     

堆型艾美球虫广东株3-1E基因的克隆与序列分析

根据GenBank中登录的堆型艾美球虫31E基因序列,设计了3条引物,以广东株堆型艾美球虫裂殖子总RNA为模板,利用反转录聚合酶链反应(RTPCR)扩增获得了31E基因部分片段,将这一片段克隆至pGEMTEasy载体中,经PCR、限制性内切酶分析和克隆片段的序列测定、比较,证实了克隆片段的可靠性。序列比较发现,所克隆的基因片段与Eimeriaacervulina美国株(US)、E.acervulinaQH株31EcDNA的核苷酸同源性分别为99.0%和99.2%,推导氨基酸的同源性分别为98.2%和97.6%。     

鸡肾型传染性支气管炎病毒的分离鉴定

从陕西省榆林市某蛋用种鸡场疑似肾型传染性支气管炎病鸡中分离到了1 株肾型IBV(定名为YL 04),并对分离病毒进行了血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定及S1基因5′端的RT PCR鉴定。结果表明,该分离株经10 g/L胰酶处理后的各代病毒尿囊收集液均可凝集鸡红细胞;标准阳性血清可特异性地抑制其凝集性;可复制出与自然发病相同的病例;病毒传代物有明显的致鸡胚矮小化作用;透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子;用RT PCR方法扩增到1 条373 bp的目的片段,其核苷酸序列与IBV CQ/01/2004株序列的同源性达98%。     

High performance liquid chromatographic determination of alcohols with reference to body distribution of methanol

A method has been developed on reverse phase high performance liquid chromatography for simultaneous determination of methyl, ethyl and isopropyl alcohols under refractive index detection using pure water as the mobile phase. A good separation has been achieved between these alcohols. Detector response was linear with a detection limit of 5 mg/100 ml. Recovery studies were performed by adding known amounts of methyl and ethyl alcohols to blood, lung and liver within the range 80–90%. The reproducibility of the results was always >90%. The quantitative distribution of methyl alcohol in postmortem body tissues and fluids has been reported in three cases of poisoning.