我国科技人力资源总量测算方法的研究

本文以我国的科技人力资源为研究对象,对中国科技人力资源发展研究报告中关于科技人力资源的测算方法进行了分析和解读,认为中国科技人力资源总量的测算需从深化理论研究、建立评价体系、构建专门的数据库这几个方面进行完善。     

论警检关系的基本特征与发展趋势

尽管世界上几个主要国家的警检关系存在三大模式的分野,但各国警察机关和检察机关之间既存在形式上的分工关系,又具有实质上的合作关系,只不过三大模式的侧重点有所不同而已。大致说来,英美模式的侧重点在审判程序,而大陆模式、中俄模式的侧重点在审前程序。但从一些国家近年来的司法改革看,三大模式在相互吸收的基础上,警检关系开始出现紧密化的发展趋势。     

浙江汉族人群16个Y-STR基因座遗传多态性调查

目的进一步完善浙江省汉族人群Y-STR基因座遗传多态性研究,为其法医学应用提供基础数据。方法应用Y-filer荧光标记复合扩增系统,对浙江汉族203名无关男性个体进行16个STR基因座的复合扩增,统计各基因座的群体遗传学参数。结果其中15个Y-STR基因座分别检出4~13个等位基因,DYS385基因座检出47种单倍型,GD值为0.3918~0.9609;观察到16个Y-STR基因座共同构成的单倍型199种,其中196种单倍型出现1次,2种出现2次,1种出现3次,累计GD值为0.9998。结论16个Y-STR基因座具有较强的个体识别能力,适合浙江法庭科学应用。     

8例利多卡因过敏性休克死的血药浓度分析

目的研究利多卡因过敏性休克死者利多卡因血药浓度及其与死因的关系。方法采用高效液相色谱法(HPLC),分别对使用利多卡因麻醉术中各项指标均正常的8例术中因利多卡因过敏性休克死者的血液和11例顺利完成手术患者的血液进行利多卡因浓度的检测,对比分析两者结果。结果 8例死者利多卡因血药浓度(1．61mg± 0．45mg／L)低于11例正常患者利多卡因血药浓度(2．44mg±0．47mg／L)。结论利多卡因过敏性休克死者血药浓度在正常值范围内,与发生过敏性休克致死无关。     

丝状霉形体山羊亚种巢式PCR检测方法的建立

根据已发表的丝状霉形体簇各成员核苷酸序列,设计合成了2对引物McF、McR和MmcF、MmcR,建立了可以鉴别丝状霉形体山羊亚种(Mycoplasma mycoidessubsp.capri,Mmc)的巢式PCR方法。特异性和敏感性试验结果显示,该方法只能对Mmc扩增出195 bp的片段,而对其他病原菌不能扩增出任何条带,它最低能够检测出10 pg的Mmc DNA,说明该方法具有良好的特异性和很高的敏感性。田间试验结果显示,对4株霉形体分离株及其病料均可扩增出Mmc特异性片段,表明,该巢式PCR方法可用于Mmc快速鉴定和流行病学调查。     

中国共产党对宗教信仰自由政策的探索历程

党的宗教信仰自由政策是在土地革命时期初步形成,抗日战争时期逐步成熟,建国初期全面发展,新时期系统完善的.土地革命时期的宗教政策具有限制"信教自由"的特点;抗日战争时期具有统战性的特点;建国初期比较突出地体现了管理的特点;新时期以来则更多地体现出相适应的特点.     

金黄色葡萄球菌黏附素FnBPB-ClFA共表达质粒的构建与原核表达

采用PCR方法对编码金黄色葡萄球菌纤黏蛋白B的D区和纤黏蛋白原凝集素A的A区基因片段进行了特异性扩增,并通过重叠延伸PCR扩增了FnBPB-ClFA串联基因,构建了克隆质粒pMD19-FnB-PB-ClFA。将该基因片段定向插入到原核表达载体pET-32a(+)中,构建了表达质粒pET-FnBPB-ClFA,并将其转入宿主菌BL21(DE3)。SDS-PAGE分析表明,在1mmol/L IPTG诱导下,在51ku处出现了与目的蛋白一致的外源蛋白带。Western-blot分析表明,所表达的蛋白与目的蛋白一致。上述结果表明,该融合基因在原核细胞中成功表达。     

Trade Measures and Climate Compliance: Institutional Interplay Between WTO and the Marrakesh Accords

This article examines the potential of trade measures to induce more climate-friendly policies, focusing on the relationship between global trade rules and the Kyoto climate regime. At the core of this interplay is the normative consistency of trade-related rules in the two regimes and any hierarchical relationship between them. The stronger clout of the WTO and its compulsory dispute settlement system suggest that issues involving competing claims would be referred to WTO bodies. Such bodies have so far been restrictive regarding the exceptions in WTO agreements to the general ban on embargoes and discrimination. The normative compatibility of the two regimes will also depend on their participatory interplay, specifically how they differentiate groups of actors as to rights and obligations. Non-members of WTO receive the least protection, and their vulnerability to climate-related trade measures is largely determined by their interdependence with states that consider employment of such measures. Among WTO members, the findings of a dispute settlement body would presumably differ depending on the status of the target under the Kyoto Protocol. A non-complier with Kyoto commitments would be more shielded than a non-party, because by joining the Kyoto regime a non-complier has exposed itself to regime-internal and less trade intrusive measures that should be exhausted first. A third dimension of interplay is linkage, or efforts to influence the regime interplay. To date there has only been moderate cross-agency coordination, but considerable attention is paid within each regime, including in the Millennium Round of trade negotiations, to the desirability of avoiding conflict between them.     

Data Protection: An Opening Door?

Swedish public law has incorporated a general rule of public access to documents, and to information in the wider sense, since the Freedom of the Press Act of 1766. On the Community level, the relationship between Regulation (EC) 45/2001 on data protection and Regulation (EC) 1049/2001 on public access to documents exemplifies the tension between the public's interest in scrutinising the administration carried on in its name, and the integrity of the individual. However, a similar tension exists between Sweden's Personal Data Act of 1998, implementing Directive 95/46, and the Freedom of the Press Act. A misuse model of data protection law, by seeking to concentrate on serious rather than technical infringements of privacy, might serve to ease the tension. The views expressed in this article are solely those of the author and are not attributable to any Community institution. This revised version was published online in August 2006 with corrections to the Cover Date.     

检测青霉素过敏性休克机体血中IgE类抗体

采用酶联免疫吸附试验(ELISA)法,检测青霉素过敏性休克豚鼠体内的IgE类抗体。结果显示:休克组血清中总IgE的量明显高于对照组(P<0.01)。用青霉噻唑蛋白(Penicilloylated Protein)作包被抗原,检测特异性IgE,以阴性参考血清的平均光密度加3个标准差作为阳性判断标准,发现过敏性休克动物血清中特异性IgE均呈阳性反应。本研究还发现:休克组动物在休克前、后特异性IgE间的差异具有高度显著性(P<0.01),虽然休克后特异性IgE略下降,但仍明显高于阳性标准。本研究认为:IgE量的变化与发生过敏性休克的关系应是:体内IgE升高并不必然导致过敏性休克,但过敏性休克的发生甚至导致死亡,应是IgE,特别是特异性IgE出现和升高的结果。