Zum Erfordernis eines Teilurteils trotz "Außerstreitstellung" des Mietzinsrückstandes

Bei Verbindung von Mietzins- und R?umungsbegehren ist ein Teilurteil auch dann zwingend zu erlassen, wenn zwar die H?he des qualifizierten Mietzinsrückstandes im darauf gestützten R?umungsprozess nicht strittig ist, aber der Mieter behauptet, er sei nach § 1096 ABGB von der Mietzinszahlung ganz oder teilweise befreit; dies gilt auch bei Au?erstreitstellung des Rückstandes der H?he nach. Das Teilurteil geht bei der Kombination von Mietzinsund R?umungsklage dem Rückstandsbeschluss zwingend vor.     

非洲猪瘟CD2v胞外区基因片段的真核表达及其多克隆抗体的制备

本研究利用真核表达系统表达非洲猪瘟病毒的CD2v胞外区蛋白,研究其免疫特性,制备多克隆抗体并研究其性质,为研发特异性单抗奠定数据基础。以非洲猪瘟病毒Wuhan2019-1株(GenBank:MN393476.1)为模板,合成CD2v胞外区基因序列,并将其克隆至真核表达载体pCAGGS载体中,获得pCAGGS-CD2v胞外区重组质粒。经酶切和测序鉴定后,将重组质粒转染293F细胞,获得重组胞外CD2v蛋白(简称His-CD2v)。用Ni-NTA柱纯化His-CD2v,并以此为抗原免疫BALB/c小鼠制备多克隆抗体,利用ELISA和间接免疫荧光对多克隆抗体进行鉴定。结果显示,(1)His-CD2v融合蛋白在293F中以可溶形式表达,SDS结果显示,相对分子质量约为35~80 ku的弥散带;(2)融合表达的His-CD2v能与阳性血清反应,与对照不反应;(3)HisCD2v制备的多抗效价最高达1∶218700,且能与阳性血清竞争性结合CD2v抗原。上述结果表明,His-CD2v融合蛋白的表达及其多抗的成功制备,为进一步筛选CD2v特异性单抗及研制竞争ELISA试剂盒,为非洲猪瘟感染与免疫鉴别方法的建立奠定了坚实的基础。     

从Napster的卷土重来看p2p版权及相关问题

P2p技术的相关利益主体之间相互博弈行为中存在一系列法律问题。彻底地解决p2p引发的版权及相关问题,只有将其商业化,建立利益共享机制,并完善相关版权法律制度。     

互联网融资创新、风险及其法律规制——以P2P网贷平台为分析视角

互联网金融作为传统金融自发改革、创新的产物,具有传统金融无可比拟的优势,但它既具备金融领域的固有风险,也具有互联网先进技术与传统金融结合而生成的新风险。对于这种风险,应该坚持"非法律规范—行政法规范—刑法规范"的调整位阶,以保护金融创新为监管的出发点。对于致力于信用担保的P2P网贷平台,应该对其资金池引入第三方托管,不应一概以非法吸收公众存款罪取缔。金融创新背景下,对金融管理秩序的垄断性保护失去了其正当性,对于非法吸收公众存款罪的解释,应该以公众财产权益这一法益为指导。     

基于SVM的汉语评论情感分类方法研究

情感分类是一项具有较大实用价值的分类技术．它可以对网上纷繁复杂的信息进行情感倾向标注．为用户提供一个简洁的总结信息，进而为人们制定决策提供帮助，然而目前针对汉语的情感分类开展的工作并不多。提出一种基于SVM机器学习的情感分类方法，并引入基于2-POS模型的句子主观性分析方法，利用SVM进行机器学习，实现汉语评论的情感分类。实验表明这种方法能够有效地判定评论信息的情感倾向。     

Political accountability,fiscal conditions and local government performance—cross‐sectional evidence from Indonesia

Why are some local governments more successful than others in managing resources and delivering services? And even more vitally, how can malfunctioning governments be reformed so that they perform their responsibilities more effectively? This article contributes to our understanding of theses overarching questions by exploring the interactions between political institutions and public sector performance in the context of decentralisation and local governance. It shows–both theoretically and empirically–that performance outcomes are determined by the extent to which people can hold their governments accountable through political institutions. The basic hypothesis underlying this research is that political accountability, either by encouraging sanctions upon non‐compliant public agents or simply by reducing the informational gap regarding government activities, will create forceful incentives for elected officials and civil servants to reduce opportunistic behaviour and improve performance. Using a cross‐sectional regression the hypothesis is empirically tested against evidence from newly empowered local governments in Indonesia. The empirical findings broadly support our hypotheses. Improved public services on the ground, both in terms of quantity and quality, require informed and well functioning decision‐making processes that allocate resources to priority areas that meet the demand of the broader community. Copyright © 2008 John Wiley & Sons, Ltd.     

4株猪瘟流行野毒株病毒结构蛋白E2基因的核苷酸序列分析

应用RT-PCR和nPCR扩增由4株甘肃省近期(1997-1998)猪瘟流行野毒株的E2基因,将其克隆到pGEMTEasy载体上,经转化、筛选、鉴定后,测出核苷核序列.4株流行毒株的E2基因核苷酸序列同源性为89.2%-99.7%,相应的氨基酸序列同源性为93.8%-99.0%.这4株流行毒株;与C-株(疫苗种毒)E2基因的核苷酸序列同源性为82.2%-84.3%,相应的氨基酸序列同源性为87.9%-90.2%,表明近期猪瘟流行毒株与C-株的gp55蛋白之间存在一定的差异.     

Sample Mining and Data Mining: Combined Real-Time and Retrospective Approaches for the Identification of Emerging Novel Psychoactive Substances

Novel psychoactive substances (NPS) are synthetic drugs that pose serious public health and safety concerns. A multitude of NPS have been identified in the United States, often implicated in forensic investigations. The most common and effective manner for identifying NPS is by use of mass spectrometry and the true utility lies within nontargeted acquisition techniques. During this study, a liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) assay was developed, validated, and implemented for forensic toxicology testing. A SCIEX TripleTOF™ 5600 + with SWATH^® acquisition was used. Resulting data were compared against an extensive library database containing more than 800 compounds. The LC-QTOF-MS assay was applied to the reanalysis of biological sample extracts to discover emergent NPS. More than 3,000 sample extracts were analyzed, and more than 20 emerging NPS were detected for the first time. Among these were isopropyl-U-47700, 3,4-methylenedioxy-U-47700, fluorofuranylfentanyl, N-methyl norfentanyl, 2F-deschloroketamine, 3,4-methylenedioxy-alpha-PHP, eutylone, and N-ethyl hexedrone.     

Making a Place for Alternative Technologies: The Case of Agricultural Bio‐Inputs in Argentina

This article characterizes the ways in which the actors in charge of designing and implementing public policies intervene to promote the emergence of alternatives to problematic technologies. It is based on a case study conducted in Argentina that focuses on initiatives to promote the development of biological agricultural inputs in the context of increasingly controversial chemical inputs. The study spotlights the political, institutional, and semantic efforts made by policy makers and public administrations to ensure these new inputs find their way into organizations and onto their agendas. Their work consists in attenuating the boundaries between chemical and biological inputs, and reducing opposition by creating categories and organizations that downplay potential dissension and highlight the possible coexistence of technological paradigms. Contrary to what the injunctions of technological substitution suggest, we show that putting alternative technologies on the public agenda depends largely on their inclusion in institutional and regulatory infrastructures originally designed for technologies that are likely to decline. More broadly, it relies on the construction of continuity between the two types of technologies.     

Determinants of Firm‐Level Lobbying and Government Responsiveness in Agricultural Biotechnology in China

Understanding what stimulates agribusiness firms to lobby the government and what makes the government responsive to lobbying are the two issues that have been discussed extensively in the debates concerning determinants of biotechnology policy. This paper examines the factors influencing agribusiness firms' lobbying and government response using econometric modeling on a new data set of 160 leading agribusiness firms in the food, feed, chemical, and seed industries in China. The results show that approximately 10% of agribusiness firms lobbied the government about biotechnology policy and regulations and over half of those that lobbied received a verbal or written acknowledgment from government agencies. Seed and feed companies are more likely to engage in lobbying than chemical companies. Owning GM patents not only has a positive impact on firms' lobbying activities, but firms with these patents are more likely to receive a government response to their lobbying efforts. The experience of selling GM products does not significantly influence lobbying activities or response from the government.