猪流感病毒广东株的分离鉴定及其特性

对广东省 2 5个猪场有呼吸道症状的 30～ 6 0日龄仔猪 ,用棉拭子采样 ,接种鸡胚分离病毒。经鉴定 ,7株为H1N1亚型毒株。对其中 3株分离株的形态学、部分理化特性和生物学特性的研究结果表明 ,病毒粒子在透射电镜下为杆状、丝状、椭圆形及圆形 ,大小为 80～ 12 0nm ;血凝素(HA)均为热不稳定型 ;均不耐热且对乙醚、氯仿敏感 ;均能凝集公鸡、豚鼠、兔、大白鼠、山羊等动物红细胞 ,对小白鼠红细胞的凝集性有所不同 ,均不凝集牛和猪红细胞 ;经绒毛尿囊腔接种 9日龄鸡胚 ,不能导致鸡胚死亡 ,EID50 分别为 10 — 6 .17/ 0 .2mL、10 - 5.83/ 0 .2mL、10 - 4.4 3/ 0 .2mL ;经鼻腔途径感染小白鼠 ,均未导致小鼠出现症状和死亡 ,接种后第 14d在部分存活的小鼠血清中可检出病毒抗体。     

细胞色素b基因序列测定进行种属鉴定应用研究

目的建立细胞色素b基因序列测定进行种属鉴定的方法。方法应用特异性引物扩增人与14种常见动物细胞色素b基因14817～15174区间长度为358bp的片段,用荧光标记自动测序技术对此片段进行序列测定,比较这些动物与人之间片段的序列差异。结果所有血痕样本均可以得到良好的测序结果,所测14种动物细胞色素b基因358bp的片段序列与人之间均存在明显的差异,最小为20.1%,最大差异为28.5%。结论测定细胞色素b基因序列进行种属鉴定特异性强、适用检材广,具有良好的应用价值。     

概率论在司法证明中的应用分析

目前 ,概率原理在司法证明中处于一种尴尬的地位。概率论无论在证据分析方面还是证明标准方面都具有普适性 ,问题在于必须科学地建构概率原理在司法证明中的操作性标准 ,信度理论或许可以为此构建做出有益的贡献。     

论有限公司股东的股权继承与股东资格继承

我国《公司法》第76条的规定应该解释为对死亡股东继承人股东资格的取得而设。可以认为,自然人股东死亡后,若公司章程没有相反的规定,其继承人或继承人的代理人可以马上向公司申请办理股东变更手续。为此,我国公司立法应该进一步明确公司有义务在合理的时间内办理股东变更手续,否则推定继承人取得股东资格。     

Direct PCR Improves the Recovery of DNA from Various Substrates

This study reports on the comparison of a standard extraction process with the direct PCR approach of processing low-level DNA swabs typical in forensic investigations. Varying concentrations of control DNA were deposited onto three commonly encountered substrates, brass, plastic, and glass, left to dry, and swabbed using premoistened DNA-free nylon FLOQswabs^™. Swabs (n = 90) were either processed using the DNA IQ^™ kit or, for direct PCR, swab fibers (~2 mm²) were added directly to the PCR with no prior extraction. A significant increase in the height of the alleles (p < 0.005) was observed when using the direct PCR approach over the extraction methodology when controlling for surface type and mass of DNA deposited. The findings indicate the potential use of direct PCR for increasing the PCR product obtained from low-template DNA samples in addition to minimizing contamination and saving resources.     

Inter-/Intra-observer Reliability of Hand Assessment Using Skin Detail: A Count-based Method

Skin detail of the hand is used in photographic comparisons, yet its reliability has not been evaluated. This study examines a count-based method for documenting skin features. In Part I, 14 individuals counted skin features on 40 color images of the hand, three of which were repeated. An average correlation value of 0.557 was obtained for interobserver assessment; values ranged from 0.545 to 0.832 for intra-observer assessment. The variation in correlation values for hands suggests that there are certain distinguishing characteristics that increase reliability. In Part II, 17 examiners assessed 20 nonrepeated grayscale images of hands by circling skin features. An average correlation value of 0.674 was obtained, but visual assessment of examiner markings suggested some examiners grouped features whereas others viewed them individually. The results suggest further research is warranted, some hands may be more suitable for comparisons, and a standardized method for examining skin features is needed.     

Is There a Correlation Between Footstep Length,Lower Extremities,and Stature?, ,

A probable correlation between stature and footstep length is expected, and consequently, the stature may be estimated from footstep length. The present research was conducted to study the correlation of footstep length with length of the lower extremities and stature. The study participants (n = 142) were asked to walk on a paper sheet with inked feet and footstep length was measured. Mean stature and lower limb length were significantly larger in males. Sex differences were not observed in the average footstep length. Average footstep length and lower limb length did not show a significant correlation among the participants. A statistically significant correlation was observed between average footstep length and stature only among females. Our observations suggest that the length of the lower limb may not be a major factor in determining the footstep length of a person and that the forensic utility of stature estimation from footstep length may be limited.     

Personality traits and party identification over time

Why do some people stably identify with a party while others do not? This study tests whether and how the direction, stability and strength of party identification are associated with big five personality traits, using panel data from a representative sample of German citizens. First, the study confirms that personality traits are related to identification with different political parties. Second, it moves beyond previous research by showing that personality traits are related to the strength and variation in party identification over time. The implications of the study for the classical perspectives on party identification, as well as the personality and politics literature, are discussed.     

Sequential vote choice: Applying a consideration set model of heterogeneous decision processes

This article analyzes the relative effects of a classic set of long-term and short-term determinants of party choice by treating voters' decision processes as a two-stage heterogeneous process. Departing from a consideration set model of voting behavior (CSM), we use panel data collected in Sweden in 2014 to analyze which voters considered voting for more than one party. To evaluate the CSM approach we estimate the relative effects of long-term and short-term determinants, for different parts of the electorate and at different stages of the decision process.Results confirm that the choice process for the ‘considering kind’ of voters is influenced by another mix of long-term and short-determinants than stable and party identified voters. Findings suggest that continued analyses of multi-stage decision making may bring new insights into electoral behavior. We argue that the core ideas of CSM – the sequential decision-making process and heterogeneity in the impact of long-term and short-term determinants – are generally applicable for analyses of voting behavior.