红细胞和血痕酸性磷酸酶(EAP)表型及广东人群EAP基因频率的调查

本文采用薄层聚丙烯酰胺凝胶等电聚焦电泳检测红细胞及血痕酸性磷酸酶表型,并对不同条件下的血痕标本进行检测,发现室温下(15～33℃)保存的110例纱布血痕7周内可全部正确分型,21例磁板血痕9周内均可正确分型;含血量≥5λl 的血痕可被正确检出 EAP 表型;日晒、水洗、发霉等因素可影响血痕 EAP 型的正确检出。同时调查了广东人群的 EAP 表型分布,基因频率为 p~a=0. 2338,p~b=0. 7662,发现 EAP 基因频率分布存在着地区差异。     

栅格特征在打印文件检验中的应用研究

目的通过对打印文字栅格特征进行实验研究,总结其稳定性和易引起特征变化的因素,为二次添加打印和换页变造文件检验探索新的思路。方法以500个常用汉字为主要研究对象,选用不同品牌、不同型号激光打印机,设置不同分辨率或质量模式,用同一台电脑或不同台电脑安装不同驱动程序,打印中易宋体常用字不同字号的样本。通过对打印文字笔画细节特征进行比对分析,总结打印文字栅格特征的规律特点。结果打印文字存在三类栅格特征,一是横笔段厚度特征,二是竖笔段宽度特征,三是斜笔画阶梯特征。同一台打印机一次打印的文件,相同字体字号文字的栅格特征反映相同;不同打印机或同一台打印机连接不同版本操作系统的电脑,或安装不同驱动程序,或选择不同的打印分辨率,栅格特征可能发生变化。结论栅格特征在同一次打印的文件中具有稳定性,在不同硬件和软件环境下可能发生改变,可以为换页文件和二次添加打印文件检验提供新的思路。     

地面三维激光扫描在交通事故勘查及重建中的应用进展

本文回顾了地面三维激光扫描技术的发展历程和应用场景,简要阐述了地面三维激光扫描系统的技术原理和作业流程,对地面三维激光扫描技术在交通事故领域的应用进行分类,并详细梳理了各类别下的最新研究成果与实例,结合交通事故勘查和重建发展需求,探讨了目前存在的问题和未来发展方向。     

Value-Added Tax (VAT) Development and Reform History—A Study of VAT Evolution Around the World and in China

Value-added Tax (VAT), a turnover tax levied on the value appreciated in commodities production, circulation and sale, has been widely practiced by economies worldwide for its neutrality. From the international perspective, European Union VAT and New Zealand VAT stand out as characteristic models of VAT development and reform history. With changing economic development models and the growth of e-commerce businesses, VAT systems have been upgraded to meet taxation challenges brought about by the digital economy. Recently, VAT policies and measures have been introduced particularly to combat the COVID-19 impact. In China, the VAT system has been reformed constantly over the past 40 years and has been converging with international practice. China now needs to address emerging VAT issues through legislation, tax incentives, and tax-rate grades so as to meet challenges in VAT collection and management and to suit the “new normal” of economic development.     

On the Legal Regulation of Algorithms

Increasingly, algorithms challenge legal regulations, and also challenge the right to explanation, personal privacy and freedom, and individual equal protection. As decision-making mechanisms for human-machine interaction, algorithms are not value-neutral and should be legally regulated. Algorithm disclosure, personal data empowerment, and anti-algorithmic discrimination are traditional regulatory methods relating to algorithms, but mechanically using these methods presents difficulties in feasibility and desirability. Algorithm disclosure faces difficulties such as technical infeasibility, meaningless disclosure, user gaming and intellectual property right infringement. And personal data empowerment faces difficulties such as personal difficulty in exercising data rights and excessive personal data empowerment, making it difficult for big data and algorithms to operate effectively. Anti-algorithmic discrimination faces difficulties such as non-machine algorithmic discrimination, impossible status neutrality, and difficult realization of social equality. Taking scenarios of algorithms lightly is the root cause of the traditional algorithm regulation path dilemma. Algorithms may differ in attributes due to specific algorithmic subjects, objects and domains involved. Therefore, algorithm regulation should be developed and employed based on a case-by-case approach to the development of accountable algorithms. Following these development principles, specific rules can be enacted to regulate algorithm disclosure, data empowerment, and anti-algorithmic discrimination.     

超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法检测血斑中乙醇生物标志物

Objective To establish a method for determination of two alcohol biomarkers (ethyl glucuronide, EtG;ethyl sulphate, EtS) in bloodstains via ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q/eOrbitrap HRMS). Methods The bloodstains were prepared on clean glass slide where to have dropped the venous blood spiked of EtG or EtS, having them extracted with 50% hydrated methanol. The bloodstain-extracted supernatant was separated on a Synicronis C18 column (150mm×2.1mm×5.0μm) with gradient elution that was run through the mobile phases consisting of 0.1% formic acid (phase A) and acetonitrile: methanol (v/v 1:1) (containing 0.1% formic acid ) (phase B). MS tests were coupled with HPLC under HESI (heating electrospray ionization) source operated in negative ionization full-scan mode. Results Both EtG and EtS were linear with their calibration curves among the range of 0.2-100ng/patch (R²=0.9991, 0.9994), showing good recovery, intra-and inter-day precision less than 15%. The matrix effect was in the range of 85%-120%. Conclusion The method is effective to detect alcohol biomarkers (EtG and EtS) in bloodstains. © 2021, Editorial Office of Forensic Science and Technology. All rights reserved.     

Use of Eucalyptus DNA profiling in a case of illegal logging

Eucalyptus is grown world-wide for paper pulp, solid wood, and other industries. Theft or illegal cutting of the trees causes hardship to owners of plantations and countries whose economies rely on the sale and export of eucalyptus products. Unfortunately, many of these crimes go unpunished due to lack of forensic evidence.Over 1200 short tandem repeat (STR) markers have been identified in the genomes of genus Eucalyptus and related species. However, their importance and utility in aiding forensic investigations of wood theft have not been explored. This study evaluated nine STRs for diversity and applied them to a case involving suspected wood theft.As expected, three dinucleotide STR markers showed greater variability but resulted in harder to interpret profiles. Four STR tetranucleotide markers evaluated in this study were found to contain additional repeat structures (dinucleotide or trinucleotide) that enhanced their variability but resulted in profiles with peaks at multiple stutter positions and heterozygote peak imbalance. The most promising STR markers were EGM37 and EMBRA 1374. Though less variable, they yielded robust and reproducible DNA profiles.All nine STR markers were applied to a case involving suspected wood theft. Samples were collected from seized wood and from remaining stumps in a plantation. No DNA match was found, thus eliminating the evidence samples as having originated from the forest. Dendrochronology analysis also resulted in an exclusion. This case study represents the first report using STR markers in any eucalyptus species to provide DNA evidence in a case of suspected wood theft.     

在新的历史起点上推动人民政协制度更加成熟定型

国家政治制度成熟定型必须具备系统完备、相对稳定、科学有效和广泛认同四个基本要件,人民政协制度更加成熟定型既要遵从上述要件,又要考虑政协性质特点,做到理论和政治上成熟,有鲜明的政治定性;职责和功能上成熟,有稳定的职能定位;制度机制成熟,有完备的工作定规。70年来人民政协制度化建设稳步推进,各项制度体系正在加速形成,新时代人民政协要更好地发挥在国家治理中专门协商机构作用,必须加快协商制度创新和完善,在新的历史起点上推动人民政协制度更加成熟定型。     

Ombudsman for Bangladesh: Theory and Reality

Abstract

Although the term “Ombudsman” is a relatively unfamiliar term in Bangladesh in general, it is widely used and is a practiced technique of ensuring administrative accountability and transparency in western developed countries. The offices of Ombudsmen, as currently found in an increasing number of countries, are institutions of government design to meet the perceived needs as to both out come and process. Their objectives are to secure fairness, integrity, accountability and efficiency in public affairs, by methods conductive to promoting confidence in all institutions of the state. In reality the Ombudsman's office is seen as an institution, which has been established in regional jurisdictions in order to promote high standards of public administration and management, and to protect the right of citizens in their dealing with government. In this article, an attempt is made to analyze the role of the “Ombudsman” concept and why it is needed and how it can be institutionalized in Bangladesh. In doing so, the article has been divided into two parts. Part I discusses the conceptual framework of the Ombudsman while part II throws light on the prospects and problems of this concept in Bangladesh as attempts are being made to establish the office of the Ombudsman.     

Shared Services and Cost Reduction Motive in the Public Sector

The intention of this article is to understand whether and how shared service centers can help reduce costs in the public sector context. We identify the sources of cost reduction for shared service centers and discuss the obstacles to making use of them. In order to illustrate and complement the theoretical discussion and the literature review, empirical insights from two Estonian cases are provided. The case studies indicate that when the context is enabling, shared service centers can help reduce back-office headcount. However, the total costs and benefits of the public-sector shared service centers are not calculated and remain unknown.