武汉汉族人群短串联重复序列D5S818， D7S820位点多态性研究

目的研究Ｄ５Ｓ８１８,Ｄ７Ｓ８２０的多态性及法医学应用价值。方法应用聚合酶链反应（ＰＣＲ）、聚丙烯酰胺凝胶电泳分离及银染显带技术对武汉地区汉族２３２例无关个体作Ｄ５Ｓ８１８,Ｄ７Ｓ８２０位点分型调查。结果Ｄ５Ｓ８１８和Ｄ７Ｓ８２０位点分别检出８个和６个等位基因,获汉族人群基因频率分布。二位点基因型频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡。位点杂合度分别为０８１２１和０７９３４,个人识别能力分别为０９４１６和０９２５５,非父排除率分别为０５８４２和０５８１６。结论Ｄ５Ｓ８１８和Ｄ７Ｓ８２０ＳＴＲ位点均是高杂合度、高鉴别能力的遗传标记系统,在法医学个人识别和亲子鉴定中有较高实用价值     

应用MVR-PCR和银染法分析河北汉族人群D1S8基因座DNA结构多态性

研究D1S8基因座重复序列内部的变异,并进行数字编码。应用MVR－PCR和聚丙烯酰胺梯度凝胶电泳银染法对240名河北汉人无关个体进行检测。结果每个个体得到约30个数字编码,未发现任何两无关个体所有编码相同,30个编码完全相同的概率为3．55×10－11。3种重复单位a-型、t-型、o-型出现的频率分别为54.77％、42．54％、2．69％。为小卫星变异重复序列的研究及其在法医实践中的应用提供了一种新方法。     

Application of non-contact scanning to forensic podiatry: A feasibility study

Foot impression evidence recovered from crime scenes can be available in the form of barefoot prints, sock-clad footprints, or as impressions within footwear. In some cases, suspects leave their footwear at the scene of the crime, and the insoles from the footwear can be important in linking a person to the footwear. The application of 3D data-collecting technology is becoming more and more popular within forensic science and has been used to recover footwear impression evidence. The present study is a feasibility study to discover if 3D data capturing devices can be applied to insoles; to capture the footprint impression for measurement using the Gunn method (a method used in forensic podiatry casework). Three different methods of data capture were conducted; Adobe Photoshop, MeshLab, and calipers used directly on the insole. Paired t-tests and Intraclass Correlation Coefficient (ICC) were conducted for all three data capture methods. Seven measurements used in this study were significantly different across all three methods. ICC scores were moderate to excellent for the Photoshop method, poor to good for the 3D method, and moderate to excellent for the Direct method.     

液相色谱-三重四级杆/线性离子阱串联质谱法测定血液中1-(4-甲氧基苯基)哌嗪

目的建立血液中1-(4-甲氧基苯基)哌嗪的液相色谱-三重四级杆/线性离子阱串联质谱(LC-QTrap-MS/MS)的快速检测方法。方法血液经乙腈沉淀蛋白后,振荡、离心、经微孔滤膜过滤后供仪器分析;选用Phenomenex Kinetx®5.0μm Biphenyl 100Å(100mm×2.1mm)分离,柱温40℃;以0.1%甲酸水溶液-乙腈为流动相,流速0.5mL/min,梯度洗脱5.5min;以多反应监测(MRM)-信息依赖性采集(IDA)-增强子离子扫描(EPI)-谱库检索的模式进行分析,外标-标准曲线法进行定量分析。结果血液中1-(4-甲氧基苯基)哌嗪的浓度在2~200ng/mL范围内呈现良好的线性关系,相关系数达到0.9984,回收率在87%以上,日内精密度在5%以下,日间精密度在10%以下,方法的检出限是0.8ng/mL,定量限是2ng/mL。结论该方法采用LC-QTrap-MS/MS和MRM-IDA-EPI模式,既保持与MRM相同的定量灵敏度,也能提供更为丰富的二级质谱碎片,使定性结果更加准确。方法简单快捷,专属性好,回收率高,重现性好,能够用于血液中1-(4-甲氧基苯基)哌嗪同时定性和定量分析。     

“大学英语”精品课程建设：以“口语”为特色

根据《大学英语课程教学要求》,本文描述了以“口语”为特色,集课堂、英语寝室、计算机网络、校园文化等“四维”为系统的大学英语精品课程建设模式,要在教学改革创新中保留并丰富“口语领先”这一多年形成的传统智慧。     

联合国全民教育和教育可持续发展目标视角下拉美教育性别平等

由于贫困、社会偏见和传统教育体制的局限性,拉美教育存在较为严重的性别不平等,未达到联合国“2000—2015年全民教育”性别均等和平等目标的要求,主要表现为中学阶段男性失学现象严重,女性面临学习环境不包容、学业中存在障碍和就业竞争力弱等问题。要解决这些问题,需要运用新的思路打开局面。联合国“2015—2030年教育可持续发展目标”的17项具体目标均提及性别平等。该目标强调包容公平和终身学习两个要点,把全民教育关于教育普及和机会均等的各个分散目标整合到上述两个主题之下,这为解决拉美教育性别不平等问题提供了一个综合的解决思路和行为框架。按照该目标的思路,拉美国家应继续实施有条件现金转移支付等公共政策,促进教育机会公平,减少男性失学现象;应努力消除校园暴力和性别歧视,实现优质包容的教育;应重视职业技术教育,为所有男性和女性在学习和工作多个阶段提供灵活的、个性化的、以就业为导向的职业教育,帮助其实现终身学习和可持续发展,促进教育质量的性别平等。     

D20S161和D8S384两个基因座在法医学中的应用

评估D2 0S16 1和D8S384两个基因座在法医学中的应用价值。用自制的D2 0S16 1和D8S384两个DNA分型试剂盒 ,对人血、人精液、人唾液、动物血、人血与动物血的混合检材和人血痕、人精液斑、人唾液斑、动物血痕、人血与动物血的混合斑痕检材 ,以及陈旧血痕检材进行检测分型 ,并用这两个基因座PCR引物序列与DNA数据库进行联网对比分析。自制的D2 0S16 1和D8S384两个DNA分型试剂盒能对人血、人精液、人唾液、人血与动物血的混合检材分型 ,而动物血没有PCR产物 ;自制的D2 0S16 1和D8S384两个DNA分型试剂盒能对人血痕、人精斑、人唾液斑和人血与动物血的混合斑痕检材正确分型 ,而动物血痕没有PCR产物 ;斑痕检材分型结果与对应体液检材分型结果无差异 ;5 0份陈旧血痕检材全部获得阳性分型结果。DNA数据库联网比较提示 ,D2 0S16 1和D8S384基因座引物除了能与各自的模板序列发生特异性扩增外 ,理论上不能与DNA数据库中 6 0 6 36 4种已知序列产生PCR产物。D2 0S16 1和D8S384两个基因座具有高度的种属特异性 ,抗污染能力强 ,不易受降解的影响 ,是解决法医现场生物检材个人识别和亲子鉴定的理想手段     

Universitätsrecht,universitäre Selbstverwaltung,Aufsicht, Satzung WU Wien

Die WU Wien ist – durch das zur Au?envertretung berufene Rektorat – zur Erhebung der Beschwerde an den VfGH legitimiert. Eine Kompetenz des BM zur "Aufhebung" von Verordnungen (hier: Bestimmung der Satzung der WU Wien) mittels Bescheid anzunehmen verbietet sich aus verfassungsrechtlichen Gründen. Verletzung des Rechts auf den gesetzlichen Richter.     

Do specific forms of university-industry knowledge transfer have different impacts on the performance of private enterprises? An empirical analysis based on Swiss firm data

This study investigates the impact of a wide spectrum of Knowledge and Technology Transfer (KTT) activities (educational and research activities, activities related with technical infrastructure, and consulting) on two innovation indicators (a) in the framework of an innovation equation with variables for specific forms of KTT activities as additional determinants of innovation, and (b) based on a matched-pairs analysis for several specific forms of KTT activities. The data used in the study were collected by means of a survey of Swiss enterprises that took place at the beginning of 2005. We found that research and educational activities improve the innovation performance of firms in terms of sales of considerably modified products, research activities in addition also in terms of sales of new products. This could be shown by several methods: the innovation equation approach with instrument variables for specific forms of KTT activities as well as two matching methods.