基于年龄相关DNA甲基化位点推断河南汉族个体年龄

目的探查中国河南汉族个体与年龄相关的DNA甲基化位点,构建年龄推断模型,进行甲基化和年龄相关性分析。方法采用焦磷酸测序法对ELOVL2、ClOrf132、KLF14、TRIM59和FHL2基因的34个CG位点进行甲基化分析,利用SPSS 23软件的多元回归方法建立模型,对甲基化和年龄相关性做分析。结果除ClOrf132基因3个CG位点的甲基化水平与年龄呈负相关外,其余4个基因的31个CG位点甲基化水平均与年龄呈正相关。多元回归分析表明,年龄与CG位点的甲基化水平存在明显的线性关系,实际年龄与推断年龄偏差在5岁以内的准确度达80%以上。结论本研究构建的河南汉族个体年龄推断模型,有助于通过检测血液等组织的DNA甲基化水平推断个体的年龄范围,具有法医学应用前景。     

Regulating for responsibility: reputation and social media

The framework brought forward by the United Kingdom's Defamation Act 2013 underlines a traditional hierarchy of expression in which news media are viewed as high-level speech. Although of a different form, social media are a dominant means of expression. The current study explores the rationale for a more robust and forceful discussion of responsibility in speech on social media platforms. The underlying premise here is that speech should be viewed as a qualified good and that a more appropriate paradigm is one found in the phrase ‘freedom to participate’.     

Self-expression and collaborative ‘pro-sumption’ in the digital brandscape

ABSTRACT

The evolution of the global economy has seen the emergence of a digital market space. The collaborative and expressive nature of interactive media and technology allows users to participate in the production and publication of branded content within digital communities of affinity. This presents increased possibilities for self-expression, as well as unlimited opportunities for participation in determining and influencing brand narratives. The digital brandscape has therefore become a collaborative space where surplus brand value is produced, but also where struggles in terms of power and counter power between brands and brand consumers evolve. Against this background, this conceptual article explores the brand challenges the notion of consumer brand self-expression poses in a collaborative open-source context where brand identity is constantly challenged, and brand narratives are continuously written and rewritten by multiple brand authors. The article concludes that it is important that brands do not navigate these risks by adopting defensive stances, but rather boldly seize the opportunities that these technologies offer for creating new market linkages and for developing customer insights that create innovative brand value and impact, by tapping into opportunities for creative self-expression.     

Nondestructive Methods for Recovery of Biological Material from Human Teeth for DNA Extraction

The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces.     

Evaluation of a Visualization Assay for Blood on Forensic Evidence

In forensics, bloodstains on dark fabrics might be invisible for the naked eye. Although several visualization, presumptive, and confirmatory blood tests have been developed, all have one or more disadvantages, especially on DNA analysis. We report here the use of a visualization assay that can visually detect blood drops up to 1/20 dilution. In this assay, the fabric is placed between two wet filter papers and covered by glass surfaces on both sides. Pressure is applied on the glass surfaces in which bloodstains transfer onto the filter papers through capillary forces. Detected stains can be tested with other more sensitive presumptive blood tests performed on the filter paper. Even more, DNA analysis can be performed on the transferred bloodstains. The presented visualization assay is easy to perform, extremely cheap, requires little hands on time, and does not affect bloodstain pattern analysis.     

Effects of Homeopathic Mother Tinctures on Breath Alcohol Testing

In some countries, it is illegal to drive with any detectable amount of alcohol in blood; in others, the legal limit is 0.5 g/L or lower. Recently, some defendants charged with driving under the influence of alcohol and have claimed that positive breath alcohol test results were due to the ingestion of homeopathic mother tinctures. These preparations are obtained by maceration, digestion, infusion, or decoction of herbal material in hydroalcoholic solvent. A series of tests were conducted to evaluate the alcoholic content of three homeopathic mother tinctures and their ability to produce inaccurate breath alcohol results. Nine of 30 subjects gave positive results (0.11–0.82 g/L) when tests were taken within 1 min after drinking mother tincture. All tests taken at least 15 min after the mother tincture consumption and resulted in alcohol-free readings. An observation period of 15–20 min prior to breath alcohol testing eliminates the possibility of false-positive results.     

An Accelerated Analytical Process for the Development of STR Profiles for Casework Samples

Significant efforts are being devoted to the development of methods enabling rapid generation of short tandem repeat (STR) profiles in order to reduce turnaround times for the delivery of human identification results from biological evidence. Some of the proposed solutions are still costly and low throughput. This study describes the optimization of an analytical process enabling the generation of complete STR profiles (single‐source or mixed profiles) for human identification in approximately 5 h. This accelerated process uses currently available reagents and standard laboratory equipment. It includes a 30‐min lysis step, a 27‐min DNA extraction using the Promega Maxwell^®16 System, DNA quantification in <1 h using the Qiagen Investigator^® Quantiplex HYres kit, fast amplification (<26 min) of the loci included in AmpF?STR^® Identifiler^®, and analysis of the profiles on the 3500‐series Genetic Analyzer. This combination of fast individual steps produces high‐quality profiling results and offers a cost‐effective alternative approach to rapid DNA analysis.     

基于裁判文书的法学实证研究之审视

知识图谱分析结果表明,基于裁判文书的法学实证研究成果数量总体上呈现增长趋势,但在近两年出现明显下降;基于裁判文书法学实证研究学术群体规模正在逐步缓慢扩大,但研究成果在研究主体间的分布呈较为集中趋势;研究成果所涉及学科领域具有较为明显的局限性,且具体研究内容伴随热点问题获得短暂爆发之后开始呈退缩之势。在方法转型背景之下,基于裁判文书法学实证研究的演变,受到研究方法在数据可得性、数据完整性以及数据客观性等方面所存在之优势与缺陷影响。研究人员自身应当在研究过程中通过丰富研究数据、规范研究方法之适用以及推动数据处理技术的发展等方式寻求基于裁判文书法学实证研究的长远发展之道。     

关于土地革命战争时期的毛泽东党建思想研究

土地革命战争时期,毛泽东在多篇重要的讲话、报告和文章中探讨了在当时的历史背号下中国共产党的自身建设问题,其内容主要涉及到党的思想建设、组织建设和作风建设等方面。进入21世纪,中国共产党人重温毛泽东土地革命战争时期的党建思想,对做好新时期的党建工作给予诸多而有益的重要历史启示。     

毛泽东文化大众化思想及其时代价值

文化大众化思想是毛泽东文化理论中极富特色的组成部分。从历史逻辑看,毛泽东文化大众化思想经历了一个初生萌芽、不断形成、逐渐成熟、继续发展的递进过程。从主要内容看,毛泽东文化大众化思想包括文化根本来源大众化、文化丰富内容的大众化、文化表现形式的大众化、文化创作主体的大众化和文化服务对象的大众化等具体内容。从时代价值而言,毛泽东文化大众化思想对于坚持社会主义先进文化的大众属性、维护广大人民群众切身的文化权益和促进当代中国大众文化的健康发展均具有重要意义。