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11.
人线粒体DNA序列分析在法医学中的应用研究及其进展 总被引:1,自引:0,他引:1
综述人线粒体DNA(m tDNA)序列分析在法医学种属鉴别、个体识别,以及个体年龄推断中的应用研究及其进展,展望对m tDNA异质性的研究及建立人m tDNA数据库,并具有重要的法医学实践意义。 相似文献
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Steffen L. Lauritzen Julia Mortera 《Forensic Science International Supplement Series》2002,130(2-3):125-126
We derive a simple inequality for the probability of observing a given DNA profile when assuming a fixed number of unknown persons have contributed to the mixed stain. We then show how this inequality can be used to obtain an upper bound for the number of unknown contributors needed to be considered. 相似文献
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《Forensic Science International: Genetics Supplement Series》2013,4(1):e29-e30
Improvised explosive devices (IEDs) are used in devastating terrorist attacks worldwide and daily in Thailand. Touch DNA deposited during IED assembly are subjected to intense heat and pressure, resulting in rare events of usable DNA profiles obtained from real casework. No study has simultaneously evaluated both swab brands and moistening agents for touch DNA collection from substrates encountered in IED evidence. In this study, we investigated the effects of swab brands and moistening agents on DNA collection from adhesive tape, a common IED substrate. A full factorial design using four cotton swab brands (two forensic and two medical cotton swabs) and six moistening agents (DNA-free water, phosphate-buffered saline, ethanol, sodium dodecyl sulfate, isopropanol, and lysis buffer) was employed (24 total combinations). Using buffy coats, we found that DNA recovery depended on both swab brands and moistening agents (p < 0.05). The optimal method recovered significantly higher DNA amount from real IED cases compared to the standard Royal Thai Police method. Percentages of high partial profiles also increased. Our results changed the standard operating protocol of the Thai police. Other commonly found substrates from IED cases are being investigated to maximize the evidential value obtained from touch DNA. 相似文献
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Darrell O. Ricke Ph.D. Philip Fremont‐Smith M.S. James Watkins B.S. Sara Stankiewicz M.S. Tara Boettcher B.S. Eric Schwoebel Ph.D. 《Journal of forensic sciences》2019,64(5):1468-1474
High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures. 相似文献
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ABSTRACT The present research examined the CSI Effect and the impact of DNA evidence on mock jurors and jury deliberations using a 3 (Crime Drama Viewing: low, moderate, high)?×?3 (Evidence: DNA innocent, DNA guilty, no DNA control) design. A sample of 178 jury-eligible college students read a case of breaking and entering. Pre-deliberation, some support for a CSI Effect was found with high viewers’ extent of guilt ratings significantly lower than moderate and low viewers’ in the no DNA control and the DNA innocent conditions. This effect was not present for verdicts. Contrary to a CSI Effect, crime drama viewing was not related to guilt judgments with incriminating DNA evidence. A content analysis of comments made during deliberations found little support for the CSI Effect entering the jury room. Specifically, CSI Effect predictions were not supported when examining the discussion of DNA evidence, expressing DNA opinions, or mentioning missing evidence. Overall, the limited CSI Effect found for individuals was attenuated during deliberation. The alarm raised over a possible CSI Effect influencing jury decision making may be unwarranted. 相似文献
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DNA侦查技术克服了以往遗传标记检测的种种缺陷以及指纹技术的一些局限 ,实现了物证检验从否定到认定的飞跃。目前DNA侦查技术正在走向更广阔、更深入的领域 ,出现了具有良好应用前景的一些新技术 ;许多国家均投资建立国家DNA犯罪数据库。我国的DNA犯罪数据库也在加紧建设中。从目前我国的有关情况看 ,建立DNA技术标准化和质量控制体系 ,已成为我国法庭科学亟待解决的问题。然而 ,DNA鉴定的不断完善 ,还有赖于人类基因组计划。 相似文献
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Hari Jang M.S. Sang Eon Shin Ph.D. Kyu Jin Youm B.S. Mustafa Zafer Karagozlu Ph.D. Chang Bae Kim Ph.D. Kwang Soo Ko M.S. Seong Hwan Park M.D. Ph.D. 《Journal of forensic sciences》2020,65(1):283-287
Species identification of necrophagous insects found on a dead body is an essential key in applying medicolegal entomology to the estimation of postmortem interval (PMI). Due to limited morphological identification of insect evidence, several studies have identified species using molecular information such as DNA markers. While considerable cytochrome c oxidase subunit I (COI) gene sequence data of necrophagous fly species have been collected and annotated, those of necrophagous beetle species have not. Since necrophagous beetles such as Dermestes species have a larval period longer than that of flies, beetles are useful in even the late decomposition phase in estimating minimum PMI. To obtain the full-length COI gene sequences of six Dermestes species collected from South Korea, we designed primers for polymerase chain reaction amplification and sequencing. The obtained full COI nucleotide sequences were used for performing phylogenic analysis and comparison with previously reported sequences. The results demonstrated that the COI gene sequences could be used to identify forensically important Dermestes species in South Korea. 相似文献