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71.
John Buckleton James Curran 《Forensic Science International: Genetics Supplement Series》2008,2(4):343-348
DNA mixture interpretation is undertaken either by calculating a LR or an exclusion probability (RMNE or its complement CPI). Debate exists as to which has the greater claim. The merits and drawbacks of the two approaches are discussed. We conclude that the two matters that appear to have real force are: (1) LRs are more difficult to present in court and (2) the RMNE statistic wastes information that should be utilised. 相似文献
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本文选择Promega公司的3个复合扩增系统对荆州地区汉族人群进行了基因频率调查,获得了9个STR基因座的群体遗传学参数,报告如下。1材料与方法1.1实验材料1.1.1样本146例汉族无关个体血样来自荆州市各县市区,系本实验室日常检案积累。1.1.2主要仪器设备eppendorf-5331型扩增仪(eppendorf公司);model-4001型电泳仪及SA-32型电泳槽(GIBCO公司)。1.1.3主要试剂Chelex-100(Bio-Rad公司);Taq酶(Promega公司);3个复合扩增试剂盒(Promega公司)。1.2实验方法1.2.1模板DNA的制备所有血样均用5%chelex-100快速提取DNA。1.2.2复合扩增扩增总体… 相似文献
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Competitive PCR assays were established for the mitochondrial DNA hypervariable region I and the human amelogenin locus. Using these assays, the copy numbers of DNA participating in PCR (amplifiable DNA) were quantified in tissues exposed to different environments. Human ribs, skin and nails were left in three exposure conditions (in the open air, in soil and in water). The amounts of amplifiable DNA in these tissues were quantified during a time period of up to two months. The amount of amplifiable DNA was well preserved in hard tissues (ribs and nails) regardless of the exposure conditions, whereas the soft tissues immersed in water showed a rapid decrease in amplifiable DNA. Strong PCR inhibition was observed in the DNA extracts obtained from buried bones. This phenomenon was clearly identified from an amplification failure of the internal standards in the competitive PCR. A preliminary examination to identify the PCR inhibitor suggested that the soil itself contributed to the inhibition. In addition, the amounts of amplifiable DNA in case samples were also investigated. 相似文献
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在实际检案中,经常遇到案发现场可获取的生物检材量微,在进行必要的种属检验、精斑确证实验、ABO血型检验等常规物证初检后,便无多余的生物检材移送DNA实验室进一步做法医DNA检验。因此,笔者通过对检案中遇到的上述微量物证检材的再行处理利用,在本实验室条件下,对其进行了TH01、HUMACTBP2、AluVpA、DIS80等位点的DNA-PCR分析,获得了良好的效果。使其在实际办案中更充分地发挥了证据作用,报告如下。1材料与方法检案中已经种属或ABO血型检验后的血痕、唾液斑(如烟蒂外层纸)浸泡凹板,加入300μl去离子水,… 相似文献
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《Science & justice》2021,61(5):542-554
This study investigates the effectiveness of forensic evidence in UK volume crime investigations. The main aim was to identify characteristics of forensic evidence that influence its effectiveness in converting detections into criminal charges, as well as to critically consider the effectiveness of a recent service level agreement (SLA) implemented by Wiltshire Police, which aimed at reducing CSI attendance. The sample consisted of 445 police recorded cases received from Wiltshire Police. Presence or absence and location-related characteristics of fingerprint, DNA, and footwear evidence were evaluated on the effectiveness of forensic evidence and examined within the contexts of different volume crimes. Results showed a high level of correlation in converting detections into criminal charges where the presence of DNA, footwear, and multiple evidence types was recorded; and a positive correlation between forensic evidence ineffectiveness and presence of fingerprints, particularly in residential burglaries. Differences between individual offence types were expressed. The most prominent feature influencing the effectiveness of forensic evidence was found to be related to the movability of the exhibit associated with the recovered evidence, with DNA recovered from non-movable items presenting the strongest effectiveness. Cases processed after the implementation of the SLA did not show significant differences in forensic evidence effectiveness as compared to cases processed prior to the SLA, however, they demonstrated a lack in effectiveness of DNA evidence. The findings of the current research provide a better understanding of the contextual influences on the potential of forensic evidence and can support improvement of crime scene screening and CSI resource deployment. 相似文献
80.
Alberto Fuentes-López Aarón Pedreño Sala Elena Romera José Galián 《Science & justice》2021,61(2):150-159
Studying dipterans at the scene of a death can provide essential information for interpreting the evidence and help to reconstruct the events happened to a corpse in the past. Molecular tools have been employed for identification at specific levels in the cases of cryptic species or poorly conserved specimens. Identification of specimens is essential in forensic entomology since each species has a specific growth rate, which determines the calculation of the minimum post mortem interval (minPMI). In addition, phylogeographic reconstruction within a species can help to differentiate the haplotypes from a geographic area, thereby helping to clarify the possible relocation of a corpse. The morphological identification of Sarcophagidae species is often difficult, especially for the females. This is an important Diptera family since some of its species are among the first to reach a corpse, especially in warm areas. In this study, we compared the sarcophagids found in human corpses in forensic cases in Alicante (southeast of Spain) with specimens collected from baited traps in the same area and surrounding provinces. In total, 189 specimens were collected, comprising 72 from forensic cases and 117 from baited traps. Molecular identification was conducted by sequencing the cox1 mitochondrial gene and analyzing the sequences using ABGD, GMYC, and BIN species delimitation methods. The median joining algorithm in the PopART program was used to construct phylogeographic networks. Eight species in the family Sarcophagidae were identified. The most widely collected species were Sarcophaga argyrostoma and Sarcophaga tibialis. The haplotype networks obtained for these species did not indicate a clear geographic distribution of haplotypes. The S. argyrostoma samples from Alcoy were clearly isolated. The results demonstrated that this method is useful for identifying Sarcophagidae samples in forensic investigations and it can be employed for minPMI estimation. 相似文献