Polymorphism analysis of Y-chromosomal haplotypes in the Chinese Han population living in the Shaanxi province of China

Population: Chinese Han population living in the Shaanxi Province of China.     

DYS391、DYS393基因座X染色体扩增对法医鉴定影响的探讨

目的探讨提高DYS391和DYS393基因座特异性扩增方法及两基因座的X染色体扩增产物对法医学鉴定结论的影响。方法DYS391和DYS393基因座采用PCR扩增、聚丙烯酰胺凝胶电泳结合银染进行分析。结果稀释模板DNA浓度和提高退火温度对提高此两Y染色体基因座特异性扩增并不明显。DYS391和DYS393的X染色体扩增产物对法医学鉴定结论有误导可能性。结论在法医学鉴定尤其是性别鉴定中应谨慎应用DYS391和DYS393基因座。     

Unterhaltsgewährung durch Einräumung eines Wohnrechtes

Die Einr?umung eines Wohnungsrechtes (Gebrauchsrechtes) an die Ehegattin kann in Erfüllung einer gesetzlichen Unterhaltsverpflichtung nicht schenkungssteuerbar aber auch schenkungssteuerbefreit erfolgen.     

太原地区汉族人群DYS390、DYS391、DYS393基因座多态性

Y 染色体STR基因座在不同人群的差异性远高于常染色体基因座[1] 。因此 ,为了获得鉴定能力高的检测系统 ,要尽可能多的分析多个STR基因座。本文作者采用复合扩增结合银染技术 ,对太原地区汉族男性人群DYS390、DYS391、DYS393基因座进行单个基因座等位基因频率和单倍型分布频率调查 ,旨在为遗传学、法医学及其他相关研究提供基础资料。1　材料与方法1 1　样本16 3例无血缘关系汉族男性个体的枸橼酸钠抗凝血 (山西医科大学第一医院提供 ) ;10例两代家系血(本室亲子鉴定案例 ) ;1例健康男性个体的肾脏、肝脏、脾脏、血液 (本院病理室提…     

A DYS438 null allele observed in two generations of a large family

Typing of Y-chromosomal STR loci using the AmpFISTR^® Yfiler kit showed a DNA profile lacking the DYS438 allele in an Austrian Caucasoid brother pair.Buccal swabs were collected from additional males of two generations and different branches of the family. All family members investigated did not show a DYS438 allele in their Yfiler profile. Using the Powerplex^® Y system an allele with dramatically reduced peak height was amplified. Subsequent sequencing of the DYS438 locus exhibited a transition upstream of the repetitive region.     

Analysis of mutations in father–son pairs with 17 Y-STR loci

We have examined 389 father/son sample pairs from U.S. Caucasians, African Americans, Hispanics and Asians using the 17 Y-STR loci in the Yfiler™ kit and observed a total of 24 differences between father and son. Thirteen mutations resulted in the gain of a repeat in the son and 11 resulted in a loss of a repeat. All samples resulted in single repeat mutations except one sample which contained a two repeat loss at Y-GATA-H4. Furthermore, two different sample pairs were found to have two mutations. An African American sample pair had a mutation at DYS458 and a second at DYS635 and an Asian sample pair had mutations at DYS439 and Y-GATA-H4.     

2个Y-STR基因座在成都汉族群体中的遗传学研究

目的　为了寻求新的适合于法医学应用的Y染色体STR基因座 ,我们调查了基因座DYS44 2和DYS44 6在成都群体中的分布。　方法　样本来自于成都地区汉族无血缘关系的个体 ,通过Chelex法提取样本DNA ,利用PCR扩增硝酸银染色方法进行分型。　结果　DYS44 2是一个四核苷酸简单重复基因座 ,而DYS44 6则为五核苷酸简单重复基因座。男性样本都出现了谱带 ,而女性样本则无PCR产物。DYS44 2基因座和DYS44 6基因座变异度分别为 :0 .6 86 7、0 .75 5 2。　结论　DYS44 2和DYS44 6是非常适合于法医学应用的STR基因座。     

Y-chromosome and autosomal STR diversity in four proximate settlements in Central Anatolia

Due to the longstanding human presence in the region and the influence of social traditions, the genetic make-up of populations currently inhabiting Turkey (Anatolia) is quite complex. To characterize the patterns of genetic diversity in rural Central Anatolian villages, we analyzed samples collected at four local settlements for variation at 17 Y-chromosome STR and 15 autosomal STR loci. The resulting data reveal considerable diversity within these settlements, as well as some structure in the paternal genetic variation, with a limited number of haplotypes being shared between the communities. These findings have important implications for forensic studies of Turkish populations.     

Sequence characterization of microvariant alleles at DYS627 and DYS458

Y chromosome short tandem repeats (Y-STRs) have been widely used in genetic applications and forensic casework. Recently, we found two intermediate alleles, the DYS627 allele 24.1 and the DYS458 allele 15.3, from Chinese Han population. The two allelic variants have not been recorded by the YHRD database. We have examined the molecular structure of these allelic variants by Sanger sequencing. The results showed that this intermediate allele at DYS627 was confirmed as 24.1, the sequence of which showed a base “A” insertion in the 13th repeat unit, and the intermediate allele at DYS458 was confirmed as 15.3, the sequence of which showed a base “G” deletion in the 12th repeat unit. This may be important for individual identification and paternal kinship testing. Besides, more allelic variants detected can be enriched in the Y-STR database.