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目的建立尿中2,4-D、2,4-DP、MCPA、MCPP等4种苯氧羧酸类除草剂的分析方法;方法液液提取分离,乙醚为提取液,DCPA为内标,硫酸正丁醇酯化衍生化,气质联用分析法分析;结果尿中4种除草剂添加样品的相对回收率在80%以上,检测限都在5ng/ml以下,对中毒兔尿样进行了分析;结论对实际发生的中毒案件分析有足够的灵敏度。 相似文献
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Christina Valgren Sara Wester Oskar Hansson 《Forensic Science International: Genetics Supplement Series》2008,1(1):76-77
Manual Chelex®-100 and organic extractions (phenol/chloroform) are used as routine methods at the Swedish National Laboratory of Forensic Science, SKL. The aim of this study was to find an automated DNA purification system to replace the organic method. The following methods were evaluated and compared to each other and to the organic method used routinely; BioRobot® EZ1 with EZ1 DNA Investigator Kit and Card (Qiagen), iPrep™ Purification Instrument with iPrep™ ChargeSwitch® Forensic Kit and Card (Invitrogen), Magnatrix™ 1200 Workstation with the Magnatrix™ gDNA Blood Kit Forensic and two different protocols; Forensic protocol A and B (Magnetic Biosolutions). Blood on fats, cotton swabs, moist snuff, paper towels and leather, post-mortem blood and muscle tissue were extracted with the different methods. DNA concentration and quality of the electropherograms were examined. Individual comparisons between the four extraction methods showed that iPrep™ and Magnatrix™ 1200 gave significantly lower mean quantities compared to BioRobot® EZ1 and the organic extraction method (p < 0.05). There were no significant differences between the latter two. BioRobot® EZ1 generated the best results and is in the process of being validated for routine analysis at SKL. 相似文献
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FTA-DNA直接提取法的研究与应用 总被引:1,自引:1,他引:0
目的建立一种简约且易于自动化操作的Whatman FTA卡血样DNA提取方法。方法取直径1.2mm FTA卡血样,分别用FTA-DNA直接提取法及FTA常规提取方法提取DNA,AB-Identifiler^TM试剂盒分别进行10山和25山体系检测比较,并筛选批量grA卡血样DNA自动化提取程序。结果200份grA卡血样分别采用2种方法提取DNA模板,25μl体系PCR-STR检测,分型结果均良好。10μl体系检测,FTA-DNA直接提取法优于FTA常规提取方法,图谱RFU值为1000~2000,采用自动化工作站运行该提取程序较手工操作DNA分型图谱均衡性更佳;采用FTA常规提取方法检测,图谱RFU值100~2000,小片段优先扩增现象明显,且有19%的样本出现丢峰现象,采用自动化工作站运行该程序对其结果改善不明显。经工作站运行FTA-DNA直接提取法自动程序及10山体系检测本2万余份FTA卡采集的血样,均获得16个STR基因座DNA分型结果。结论本文建立的FTA-DNA直接提取法适用于FTA卡血样自动化DNA建库。 相似文献
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石超英 《胜利油田党校学报》2008,21(2):116-118
《论语》的基本精神“仁、义、札、智、信”,不仅是伦理之道,而且也是经营管理之道。2007年是胜利油田的“和谐文化建设年”,胜利油田采油院把学《论语》引入到企业和谐文化建设中来。《论语》赋予采油院和谐文化建设新的生命力与形式。 相似文献
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In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improve DNA extraction efficiency. Fingernails (5 mg) provided by ten volunteers were used as samples. Nail pieces (1–3 pieces), shredded with nail clippers, were thinly stretched by applying 2 t of pressure to each piece using a hydraulic press. DNA was extracted by incubation at 56 °C for 10 min and 1 h during proteolysis. DNA yield from the nails pretreated using the HONMA method increased by 0.20–7.10 times compared with that from unprocessed nails. In particular, 10-min incubation using the HONMA method resulted in an average 2.05-fold increase in DNA yield compared with that under overnight incubation. However, the impact of using the HONMA method varied widely among individuals, and the amount of extracted DNA decreased in some cases, suggesting that the yield may differ depending on the nail quality. 相似文献