DNase-I纯化结合碱性裂解法提取混合斑精子DNA

目的研究脱氧核糖核酸酶I(DNase-I)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用。方法收集79份性犯罪案件混合斑检材,分别用DNase-I纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果。结果应用DNase-I纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉。结论DNase-I纯化结合碱性裂解法提取混合斑精子DNA可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定。     

试论国际环境法的共同但有区别的责任原则

共同但有区别的责任原则主要包涵两层意思:共同责任和区别责任。但共同责任和区别责任是一个不可分割的有机联系的统一整体,并分别具有丰富的内涵。确立共同但有区别的责任原则是国际环境保护事业的历史发展所使然。由于该原则体现了发展中国家维护自身权益和平等地参与国际谈判以解决全球环境问题的意愿,故而其对发展中国家具有较强的亲和力。该原则具有很强的实践品格,其适用范围主要局限于环境保护领域。它是正在形成和发展中的国际环境法的基本原则,也许在不远的将来能获得国际习惯法的法律地位和效力。     

Evaluation of the Differex™ System

Differential extraction is an efficient method to separate sperm cells from epithelial cells. A manual Chelex^®-100 based method is used at the Swedish National Laboratory of Forensic Science, SKL. The Differex™ System (Promega) uses a Proteinase K digestion of epithelial cells followed by centrifugation and phase separation. The sperm- and epithelial fractions are further purified with DNA IQ™ System (Promega) or with phenol/chloroform. The Differex™ System in combination with DNA IQ™ System were evaluated and compared to the Chelex^®-100 method used routinely. After modifications, the Differex™ System gave comparable results to the Chelex^®-100 method. The modifications included additional Proteinase K and DTT, longer incubation time and additional steps when removing the solid support from the Digestion Solution. In the Chelex^®-100 based method microscopic examination is done on the sperm pellet in a total volume of 50 μl. It was not possible to do a microscopic examination in less than 100 μl using the Differex™ System. Additionally the sperms were in clusters of epithelial cell debris. Microscopic examination is an important part of the differential extraction at SKL. Therefore, the Differex™ System will not be implemented at our laboratory.     

差示分光光度法测定双氯灭痛片的含量

目的：采用差示分光光度法测定双氯灭痛片剂的含量，方法：在波长２９０ｎｍ处，以双氯灭痛在０．０１ｍｏｌ／Ｌ氢氧化钠溶液为参比液，测其在乙醇中的差示吸收值，结果：双氯灭痛浓度在１１～５０μｇ／ｍｌ范围内△Ａ与溶液浓度呈良好线性关系（ｒ＝０．９９９６）平均回收率为１００．６％，ＲＳＤ为０．６４％，结论：该法简便，快速，准确。     

The Effects of Mixing Offenders with Non-Offenders: Findings from a Danish Quasi-Experiment

This study examines the effects of mixing offenders with non-offenders at Skejby half-way house in Denmark. Skejby half-way house constitutes an outstanding social experiment, as offenders are deliberately mixed with non-offenders in order to reduce their risk of recidivism. The treatment group consists of offenders who have stayed at Skejby half-way house while the controls are selected from residents of four other half-way houses. A Cox regression analysis reveals that the probability of reoffending is 21% lower for the treatment group than it is for the control group.     

补肾方含药血清处理的大鼠成骨细胞miRNA差异表达

目的 筛选补肾方含药血清干预的大鼠成骨细胞中差异表达的miRNA,为补肾方治疗骨质疏松症提供可供选择的目标miRNA,以探讨其通过调控靶基因表达发挥治疗效应的机制。方法 经细胞性状鉴定的原代大鼠成骨细胞用含药血清干预,采用miRNA芯片检测补肾方含药血清干预3 d与干预1 d的成骨细胞miRNA表达水平,并挑选差异表达2倍以上的miRNA进行定量PCR验证。结果 培养的原代细胞具有典型的成骨细胞性状特征。与对照血清相比,补肾方含药血清干预3 d的成骨细胞共有24个2倍以上表达差异的miRNA,其中上调的16个,下调的8个。差异表达2倍以上的miRNA定量PCR结果与芯片数据一致。结论 补肾方通过成骨细胞中差异表达的miRNA调控靶基因改善骨质疏松症。