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51.
用RT PCR技术对猪瘟兔化弱毒C株、石门强毒株 (Shimen)、近期地方流行的属于第 1群毒株的新疆毒株 (XJ)和第 2群代表毒株甘肃临洮 (LT)株E2基因的主要抗原区进行扩增 ,均得到约 5 6 1bp的基因片段 ;经克隆测序及序列分析 ,该基因编码 187个氨基酸残基 ,预计蛋白分子质量为 2 1.7ku。将这 4个基因分别克隆到原核表达载体 pGEX 4T 1中 ,经酶切、PCR扩增和测序分析确证其正确插入到表达载体中 ,阅读框是正确的 ,从而构建成重组表达载体。重组质粒转化至宿主菌BL2 1(DE3)中 ,用异丙基硫代 β D 半乳糖苷 (IPTG )进行诱导表达 ,对表达的蛋白用SDS PAGE电泳、免疫印迹和薄层凝胶扫描分析。结果表明 ,E2基因的主要抗原区可以在大肠埃希氏菌中表达 ,表达的蛋白多以包涵体形式存在 ,表达产物的分子质量约为 5 0 .0ku ,与理论推测的分子质量一致 ;薄层凝胶扫描分析表明 ,表达蛋白约占菌体蛋白总量的 2 0 .1% ;免疫印迹结果证明 ,表达的E2蛋白可被猪瘟阳性血清所识别。 相似文献
52.
Gómez MA Peñaherrera MS Aguirre-Tello V Vela-Cavinato M Giovambattista G 《Journal of forensic sciences》2008,53(2):510-511
POPULATION: Quito City population (Ecuador, South America, n = 116-207). 相似文献
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Susan S. Hetzel B.A. 《Journal of forensic sciences》2015,60(Z1):S197-S206
The regulations for gasoline's content vary depending on the time of year and physical location within the United States while the refinery and distribution system mixes product batches; this results in variability of content. ASTM E1618 requires both the aromatic and alkane EIP patterns of gasoline to compare with references. A survey was conducted by collecting gasoline from Florida to Oregon, from 85 to 93 octane. Samples were analyzed in accordance with ASTM E1618 in various states of evaporation. The range of differences found in the 90% evaporated alkane EIPs is presented and showed a continuum of response when the n-alkane response was compared with the branched alkane response. Similarly, the ratio of the alkane EIP to the aromatic EIP also showed a continuum of response at the 90% evaporated state. Gasoline samples with unusual characteristics are also discussed. 相似文献
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Seán Molloy 《International Journal of Politics, Culture, and Society》2003,17(2):279-306
This article examines the appeal of Carr's theory of international relations, which has enjoyed a longevity matched by few other bodies of work in a discipline characterised by faddism. I attribute Carr's success as an international theorist to his subtle use of philosophy, history, and political theory. Carr's holistic and interdisciplinary approach achieves its best expression in his employment of a critical dialectics of international theory in The Twenty Years' Crisis. The article does not confine itself to this element of Carr's writings as I also examine several works, such as The Future of Nations. Independence or Interdependence?, Conditions of Peace, and Nationalism and After,that consider the transformation of the international society. The latter works also employ a dialectic of power and morality in order to envision a world very different from the international system that Carr observed, an international order that approximates the emerging relations among states at the beginning of the twenty-first century. The article concludes with an examination of the role relativism played in Carr's theory scheme and the extent to which his dialectics of utopianism and realism is an important precursor of critical and postmodern theories of international relations. 相似文献
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142例过敏性休克死亡法医病理学分析 总被引:1,自引:0,他引:1
目的 探讨过敏性休克死亡案例的特点.方法 对142例过敏性休克死亡案例进行回顾性分析,并对过敏性休克死亡案例与62例非过敏性休克死亡案例的血清IgE水平进行统计分析.结果 过敏性休克死亡大多发生于医疗机构,占77.46%.采用单纯静脉给药方式致过敏性休克死亡案例占53.53%.β-内酰胺类抗生素、糖皮质激素类药物、中药制剂在过敏性休克死亡案例生前治疗药物中占有重要比例.过敏性休克死亡案例多无特异性组织病理学改变,与非过敏性休克死亡组的血清IgE水平的差异具有统计学意义(P<0.05).结论 过敏性休克死亡案例死因鉴定应根据案情、解剖检验结果及血清IgE水平等检测指标进行综合分析判定. 相似文献
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《Global Crime》2013,14(4):311-323
This paper employs the modified Wald (MWALD) causality test to re-examine the relationship between crime and its determinants (inflation and unemployment) in the United States from 1960 to 2005. Bounds test approach is employed to investigate the existence of a long-run relationship. The empirical evidence suggests that inflation and crime rates are cointegrated with a positive relationship. Moreover, the causal link is from inflation and unemployment to crime. 相似文献