血液和尿液样品中海洛因代谢物稳定性研究

目的对尿液和血液中海洛因代谢物3-β-D-葡萄糖醛酸吗啡（M3G）,吗啡,O6-单乙酰吗啡（O6）在180d内的稳定性进行研究。方法准备空白添加血液、尿液、染毒动物（大白兔）血液、尿液和吸食海洛因者血液、尿液样本,分别置于20℃、4℃、-20℃下,分别于0、1、2、4、7、14、28、56、112、156、180d时间点测定样品中M3G、吗啡,O6相对含量。结果在3种不同温度下,随保存时间的延长,血液、尿液中的O6含量均逐渐下降至零;血液中吗啡含量升高（空白血液添加组）或下降（染毒动物组）,在尿液则均升高;血液样中M3G含量均升高,尿样中则略有下降。下降和升高的幅度均随保存温度的下降而缩小。结论海洛因代谢物在-20℃时保存稳定性最佳。     

30个InDel位点在华东汉族和畲族人群的法医学应用

目的 评估Investigator（R） DIPplex试剂盒中30个插入/缺失（insertion/deletion,InDel）多态性位点在华东汉族和畲族人群的法医学应用价值.方法 采用Investigator（R） DIPplex试剂盒对华东地区565名汉族和119名畲族无关个体进行分型检测,统计分析30个位点的等位基因频率和群体遗传学参数.结果 在华东汉族人群中平均Ho为0.413 3,平均DP为0.551 1,平均PIC为0.320 0.在畲族人群中平均Ho为0.389 6,平均DP为0.543 3,平均PIC为0.3100.30个位点在汉族、畲族人群中均符合Hardy-Weinberg平衡（P＞0.05）.结论 Investigator（R） DIPplex试剂盒包含的30个位点在华东汉族和畲族中多态性良好,在特殊亲权鉴定案件中可作为良好的补充体系.     

Distinguishing the viewers,downloaders, and exchangers of Internet child pornography by individual differences: Preliminary findings

The current study assessed the relationship between personality and cognitive differences and the respondents' level of engagement in child pornography (searching for/viewing, downloading, or exchanging). 273 respondents completed the anonymous online survey; 257 respondents (94%) were classified as non-child pornography consumers, and 16 respondents (6%) were classified as Internet child pornography consumers. When only considering the child pornography users, 63% (n = 10) were categorized as searchers/viewers, 18.5% (n = 3) were categorized as downloaders, and 18.5% (n = 3) were categorized as exchangers. When discriminating between child pornography consumers, those individuals who only searched for and viewed child pornography were less extraverted than those individuals who reported exchanging Internet child pornography. Higher scores on extraversion may reflect the offender's ability to socially network on the Internet in order to gain access to child pornography (i.e., exchanging/sharing). Significant correlations also suggested a relationship between child pornography behaviors, conscientiousness, and excitement-seeking. Future research suggestions and limitations are discussed.     

Interaction of 3,4-methylenedioxymethamphetamine and methamphetamine during metabolism by in vitro human metabolic enzymes and in rats

Illicit amphetamine-type stimulant (ATS) tablets commonly contain one or more active ingredients, which have hallucinogenic and/or stimulant effects. Because components such as 3,4-methylenedioxymethamphetamine (MDMA) and methamphetamine (MA) in ATS tablets have similar chemical structures, they could be metabolized by common metabolic enzymes. To investigate potential metabolic interactions of ATS tablet components, we studied the in vitro metabolism of MDMA and MA using human metabolic enzymes. MDMA and MA were mainly metabolized by cytochrome P450 2D6 (CYP2D6) and mutually inhibited the production of their main metabolites. In vivo experiments were also performed using intravenous administration of MDMA, MA, or their mixture to rats. The plasma concentrations of MDMA and MA after co-administration were higher than those after administration of MDMA or MA alone. The results in this study imply that multiple components in ATS tablets can interact to mutually inhibit their metabolism and potentially enhance the toxicity of each component.     

Comparison of bulk and compound-specific δ13C isotope ratio analyses for the discrimination between cannabis samples

Five marijuana samples were compared using bulk isotope analysis compound-specific isotope ratio analysis of the extracted cannabinoids. Owing to the age of our cannabis samples, four of the five samples were compared using the isotope ratios of cannabinol (CBN), a stable degradation product of Δ(9)-tetrahydrocannabinol (THC). Bulk δ(13)C isotope analysis discriminated between all five samples at the 95% confidence level. Compound-specific δ(13)C isotope analysis could not distinguish between one pair of the five samples at the 95% confidence level. All the measured cannabinoids showed significant depletion in (13)C relative to bulk isotope values; the isotope ratios for THC, CBN, and cannabidiol were on average 1.6‰, 1.7‰, and 2.2‰ more negative than the bulk values, respectively. A more detailed investigation needs to be conducted to assess the degree fractionation between the different cannabinoids, especially after aging.     

Personal identification by the comparison of facial profiles: testing the reliability of a high-resolution 3D-2D comparison model

Identification from video surveillance systems is frequently requested in forensic practice. The "3D-2D" comparison has proven to be reliable in assessing identification but still requires standardization; this study concerns the validation of the 3D-2D profile comparison. The 3D models of the faces of five individuals were compared with photographs from the same subjects as well as from another 45 individuals. The difference in area and distance between maxima (glabella, tip of nose, fore point of upper and lower lips, pogonion) and minima points (selion, subnasale, stomion, suprapogonion) were measured. The highest difference in area between the 3D model and the 2D image was between 43 and 133 mm(2) in the five matches, always greater than 157 mm(2) in mismatches; the mean distance between the points was greater than 1.96 mm in mismatches, <1.9 mm in five matches (p < 0.05). These results indicate that this difference in areas may point toward a manner of distinguishing "correct" from "incorrect" matches.     

AGCU Mini系统在法医学中的应用

目的考察及评价AGCU Mini系统在法医学实践中的应用价值。方法应用AGCU Mini系统检测12 775份陈旧血样,进行梯度DNA模板浓度分析,并与IdentifilerTM试剂盒检测结果进行比对,评价体系的检测成功率及方法的灵敏度。针对AGCU Mini系统中D19S253基因座,对699份浙江汉族无关个体进行多态性调查。结果 12 775份陈旧血样采用AGCU Mini试剂盒检测,12 885份(96.1%)分型成功,检测灵敏度为40pg(10μL体系),方法成功率及灵敏度均高于IdentifilerTM试剂盒。D19S253基因座共检出9个等位基因,频率范围为0.005 7～0.316 2,杂合度为0.814 0,多态性信息含量为0.772 9。结论 AGCU Mini系统可用于法医微量物证的STR分析,与相关试剂盒联合使用价值更高。     

渤海油田漏油事故法律问题分析

渤海油田漏油事故造成了极为严重的环境污染,对海洋生态环境、海洋资源均产生严重影响。在此事件中,海洋局并未及时主动公开这一突发性的环境污染事件,应依法承担相应的行政责任,而事故责任人应承担相应的民事责任,包括但不限于清理油污、赔偿海洋生态损害、渔业资源损害、渔民损失等,海洋局和渔业部门可代表国家对事故责任人提供环境民事公益诉讼。检察机关亦应追究事故责任人的刑事责任。     

Abweisung des Grundbuchsgesuchs wegen Ungereimtheiten in den vorgelegten Urkunden und Zweifeln an der ausdrücklichen Erklärung des Verkäufers

Das geh?ufte Auftreten von Ungereimtheiten (hier: Nichtübereinstimmung der Geburtsdaten des Verk?ufers in den vorgelegten Urkunden und unzutreffende Gesamtfl?che) in Kombination mit dem Inhalt einer Spezialvollmacht, der den Machthabern in ungew?hnlicher Weise v?llig freie Hand bei der Ver?u?erung der Liegenschaft einr?umt und keinerlei Schranken zur Wahrung der Interessen des Verk?ufers vorsieht, verlangt nach einem strengen Ma?stab bei der Prüfung nach § 94 GBG. Rechtfertigen die M?ngel der vorgelegten Urkunden bei einer im Rahmen der vorzunehmenden Ermessensausübung geforderten Gesamtschau schwerwiegende Bedenken im dargestellten Sinn und bleiben daher Zweifel an der ausdrücklichen Erkl?rung desjenigen, dessen (hier: des Verk?ufers) Recht beschr?nkt belastet oder aufgehoben werden soll, dass er in die Einverleibung einwillige, muss dies zur Abweisung des Grundbuchsgesuchs führen.     

东盟三国农业发展的特点与战略举措

与其他发展中国家相比,东盟三国的农业发展具有增长速度较快、农业种植业结构多元化、农业架构比较合理等特点。东盟三国政府支持农业稳定发展的战略性举措,在早期主要是开拓土地耕种面积和土地改革,中期主要是推行绿色革命和促进农村农业企业的发展,近期注重持续农业的发展。东盟三国政府提供较为完善的农业信贷机制也是农业能够得到稳定发展的要因。