副鸡嗜血杆菌基因文库的构建与筛选

为了筛选副鸡嗜血杆菌的体内表达基因,提取了副鸡嗜血杆菌的全基因组,构建了副鸡嗜血杆菌基因组的pET系统表达文库。运用PCR及核酸内切酶(SalⅠ+NdeⅠ)鉴定基因文库,并以病原菌吸附后的康复血清作为探针,采用菌落原位杂交的方法对基因文库进行筛选。结果显示,重组质粒中有0.5～2kb的片段插入,99%的基因包含在基因文库中;重复筛选后得到的阳性克隆再经过PCR与SalⅠ+NdeⅠ酶切鉴定后定向测序,并对测序结果在NCBI上进行分析后发现筛选获得的基因中,有1个表达为转运谷氨酰还原酶、1个表达为转录终止因子,1个表达为荚膜合成域2,还有2个表达为保守假想蛋白。结果表明,本研究应用体内诱导抗原技术(IVIAT)筛选到了一些副鸡嗜血杆菌体内诱导表达基因,并对基因的功能做了初步探讨,在找寻副鸡嗜血杆菌在体内生存以及致病关键基因的道路上前进了一步,为传染性鼻炎的预防和治疗积累了有价值的资料。     

LC-MS/MS-MRM筛选血液中22种有毒生物碱成分

目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI )、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。     

The price of non-compliance with the Kyoto Protocol: The remarkable case of Norway

One of the ways to induce compliance is for an international enforcement mechanism to authorize the use of punitive consequences against a non-compliant country. However, such consequences should not cause significant damage to other (compliant) countries. The compliance mechanism of the Kyoto Protocol fails to meet this requirement. The Enforcement Branch of the Compliance Committee is instructed to impose punitive consequences on a non-compliant country that will have considerable adverse welfare effects for compliant countries as well. Using a numerical model, we show that in the case of Norway, the welfare effects can actually be worse if another country is punished than if Norway itself is punished.

Indoor Wet Screening of Exhumed Skeletal Remains: A Suggested Procedure for the Preparation of Fragile Evidence for Anthropological Analysis

Abstract: The 2007 exhumation of three children’s graves, located in rural upstate New York and dating to 1979 and 1980, was warranted as their mother had come under suspicion for the death of a child she had been babysitting in late 2006. The local March weather conditions had been wet, and heavy rains fell during the 2‐day process of casket removal. The extremely wet soil and the poor preservation of two wooden caskets increased the likelihood of damage to evidence. After remains’ transport to the forensic center, an indoor wet‐screening station was established so that skeletal elements could be (i) separated from soil matrix and (ii) preserved carefully for analysis. Not only were the remains relatively small and fragile in comparison with those of an adult, but two of the three remains were known to be fire damaged, thus the use of special laboratory preparation techniques was crucial.     

“商法通则”三大基本制度研究

目前,法学界对于"商法通则"的制定问题已经展开了讨论,但是,其中大部分的研究还只是停留在诸如是否要制定"商法通则",以及:"商法通则"的基本框架结构的层面上,而较少涉及"商法通则"所应规范的基本制度的研究.在"商法通则"所应规范的基本制度方面谈一些自己的意见,仅供立法者和研究者参考.     

Detection of Brodifacoum and other Rodenticides in Drug Mixtures using Thermal Desorption Direct Analysis in Real Time Mass Spectrometry (TD‐DART‐MS)

In recent months, there has been increased reporting of seized drug and toxicology cases containing rodenticides, the active ingredient in rat poisons. Seeing as rodenticides are not scheduled substances, they are not commonly screened for in seized drug analysis. This work investigates the use of TD‐DART‐MS for the simultaneous detection of rodenticides and drugs. Six rodenticides were evaluated, an optimal method was established, and limits of detection in the tens of nanograms were calculated. Additional studies highlight that detection at less than 1% by weight in mixtures with AB‐FUBINACA, cocaine, heroin, or methamphetamine was possible. This work presents an optimized method for detection of these compounds, allowing for the simultaneous detection of drugs and rodenticides, providing drug chemists with a tool for rapid identification of these compounds for forensic or public health purposes.     

中国民法典的三个创新

新中国第一部以法典命名的法律终于颁布施行了.建国70多年以来,民法典先后经历了五次的起草编纂,这一次我们终于把我国这么多年来现行的民商事法律法规编纂成一部法典.民法典在编纂上既没有采取德国五编制或法国三编制,而是采取了中国特色的七编制,单独设立了人格权编、侵权责任编,摈弃债法总则.民法典内容上的创新也不少.民事主体采用...     

关于证券欺诈的诉讼时效研究

目前,我国关于证券欺诈类案件的诉讼时效适用于《民法通则》的一般诉讼时效为2年,最长时效不超过20年的规定,这种做法对复杂多变的证券交易行为不太适宜。本文在研究了国外证券发达国家有关证券诉讼时效的法律规定后,提出应采取不同于《民法通则》的规定,规定证券诉讼特别诉讼时效为1年,最长时效不超过5年,对特别情况可做例外规定。     

马来西亚政党政治的变化及其走向

巫统一党独大是马来西亚长期以来政党政治的一大特点 ,但是 1999年末马来西亚第 10次大选却表明这种局面正在发生明显的变化 ,这必将对马来西亚的政局产生深远的影响。本文试从马来西亚政党政治变化入手 ,分析变化的成因 ,并对今后一个时期马来西亚政党政治和政局的发展趋势作一展望。     

论习近平的大局观

习近平的大局观是中国共产党大局观的重要组成部分。习近平总书记的"两个大局"致力于从全球发展和世纪伟业的高度来解决中华民族前途命运问题。把握这"两个大局"还必须与总体布局、战略布局、新发展格局贯通起来,放到中国特色社会主义事业发展的整体中去考虑。