Genetic polymorphisms of 20 STR loci in Chinese Han population in Tianjin,North China

The PowerPlex^® 21 System PCR Amplification Kit was a new PCR Amplification Kit developed for forensic laboratories, but there was a lack of data about this kit in Chinese people in Tianjin, North China. This kit contained 20 STR loci, D3S1358, D1S1656, D6S1043, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433 and FGA. In order to evaluate this kit and to get basic population data for its use in forensic practice in Chinese Han population, 360 unrelated Chinese Han individuals from Tianjin were typed using the Kit. Allele frequencies of the 20 STR loci and further population forensic genetic parameters were obtained. The observed genotype frequencies and expected genotype frequencies were evaluated by χ² test. No significant deviation from the Hardy–Weinberg equilibrium was observed in the population sample for the 20 STR loci. The population data in the present study can be used for routine forensic practice in Tianjin, North China.     

青岛地区汉族人群13个STR基因座的频率分布及法医学应用

目的　调查青岛地区汉族人群无关个体的 13个STR基因座 (D3S135 8、VWA、FGA、D8S1179、D2 1S11、D18S5 1、D5S818、D13S317、D7S82 0、D16S5 39、TH0 1、TPOX、CSFIPO)的基因频率分布 ,研究其遗传多态性及其在法医学个体识别及亲子鉴定中的应用价值。　方法　用美国ABI - 310型遗传分析仪对ProfilerPlus和Cofiler两个系统的 13个STR基因座的复合扩增产物进行毛细管电泳及四色荧光自动分析检测 ,基因分型软件为GeneScanv3.1和Genotyperv2 .5 .2。　 结果　获得 13个STR基因座在青岛地区汉族人群的基因频率分布数据 ,13个STR基因座的PIC >0 .5 ,DP >0 .71,CCE =0 .999999,TDP值接近 1,TPm =1.2× 10 -14 ,家系调查符合孟德尔遗传规律。　结论　ProfilerPlus和Cofiler两个系统的 13个STR基因座在法医学个体识别及亲子鉴定中具有较高的应用价值。     

Property in Body Parts and Products of the Human Body

An intriguing question, which until recently had not been directly explored by the courts, is the extent to which English law recognises body parts and products of the human body as property capable of ownership. Although the common law currently recognises no general property in a dead body (and only limited possessory rights in respect of it), this apparent “no-property rule” provides no justification, it is submitted, for denying proprietary status to parts or products of a living human body. The recent decision of the Court of Appeal in Yearworth v. North Bristol NHS Trust ([2009] EWCA Civ 37) lends strong support to the view that genetic material (as the product of a living human body) is capable of ownership, at least in the context of a claim in the tort of negligence and bailment. This article examines the various issues by reference to both English and Commonwealth authority.

广西地区3个群体15个STR基因座频率多态性

目的调查广西地区仡佬族、仫佬族、瑶族无关个体的15个STR基因座(D8S1179、1321S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法 应用AmpFISTR IdentifilerTM荧光标记复合扩增系统,对314例仡佬族、332例仫佬族、238例瑶族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI 3100遗传分析仪对扩增产物进行检测,GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果IdertifilerTM系统的15个STR基因座在仡佬、仫佬、瑶族的累积偶合率为1.839×10-16~5.073×10-17,累积非父排除率为0.9999983~0.9999991。结论该15个STR基因座足可满足仡佬族、仫佬族、瑶族法医学的个体识别及亲权鉴定的需要。     

11个Y—STR基因座遗传多态性研究

目的 调查11个Y染色体STR基因座的遗传多态性。方法 利用PCR扩增和聚丙烯酰胺凝胶电泳技术对广州地区汉族群体无关男性血样进行11个Y—STR基因座的分型。结果 11个Y—STR基因座在广州地区汉族群体分别发现3～5个等位基因,GD值最低为0.3037(DYS434),最高为0.8455(DYS390)。结论 11个Y—STR基因座在广州地区汉族群体均具有较高的遗传多态性,可应用于法科学个体识别和亲子鉴定。     

胰岛素对原代培养大鼠前体脂肪细胞增殖分化的影响

对分离自20日龄雄性SD大鼠附睾和肾周白色脂肪组织的前体脂肪细胞进行原代培养,并采用MTT比色法、油红O染色提取法分析了100~400 nmol/L胰岛素对其增殖、分化的影响,同时利用半定量RT-PCR分析了该浓度胰岛素对分化标志基因———脂肪酸合酶(FAS)、乙酰CoA羧化酶α(ACC1)基因mRNA表达的影响。结果显示,胰岛素能有效地促进前体脂肪细胞的增殖和分化(P<0.05),在400 nmol/L以下具有剂量依赖性;胰岛素处理72 h能显著提高脂肪细胞FAS和ACC1基因mRNA的表达水平(P<0.05)。证实胰岛素对原代培养大鼠前体脂肪细胞增殖分化有明显促进作用。     

亲权鉴定若干问题综述

对亲权鉴定中遗传标记的研究历史及现状进行了综述，并对国际法医遗传学会亲子鉴定委员会关于DNA生物计算的建议进行了解读。     

Population genetic data of five X-chromosomal loci in Bauru (São Paulo, Brazil)

Genetic population data for five X-STR (DXS6854, DXS7424, DXS101, DXS6808 and DXS7132) were obtained from Bauru population (São Paulo, Brazil). No deviations from the Hardy-Weinberg equilibrium were observed, with the exception of DXS101. The combined powers of discrimination in males and females were 0.99897253 and 0.99999120, respectively. These high values show the potential of this system in human identification and paternity testing.     

Genetic analysis instrumentation innovations built into the SeqStudio ™ Flex Genetic Analyzers

The new Applied Biosystems™ SeqStudio ™ Flex Series Genetic Analyzer have improved the benchmark for research use only for Capillary Electrophoresis (CE) by providing innovative approaches to enhanced hand-free operation, flexibility, ease of use, data quality and connectivity. This newly designed 8 or 24 capillary system supports fragment sizing and DNA sequencing applications providing scientists with medium throughput technology for use in research applications. The steps from system set-up to size or base-called data have been simplified with hardware functionality and user-friendly software enhancements designed into this new CE system.We will discuss innovations related to ease of use including one button start-up, an on-board computer with touchscreen, intuitive software, easier to use capillary arrays, and desktop and cloud-based plate manager software. Innovations related to increased flexibility include continuous plate loading, automated plate linking that be able to maintain traceability from sample to result when using barcoded plates, urgent sample reprioritization, fragment and sequencing samples be run on the same plate, and multi-user support will also be discussed. In addition, gold standard fragment analysis and sequencing data quality has been enhanced through innovative algorithms providing autospectral calibrations, and off-scale recovery of data for fragment analysis. Innovative service and support functionality including remote troubleshooting with instrument system login capability, and on-board instrument help videos are included. Finally, we will touch upon new connectivity, which includes Thermo Fisher connect for remote monitoring, analysis, and data sharing as well as other functionality such as voice commands and Wi-Fi capability that the system will provide. The following summarizes highlights from the developmental validation performed to demonstrate the functionality of SeqStudio ™ Flex Series Genetic Analyzer.     

岫岩满族9个STR基因座遗传多态性

目的用复合荧光扩增体系调查辽宁鞍山岫岩满族无关个体D6S1043、D7S3048、D9S925、D11S2368、D14S608、D15S659、D17S1290、D20S470和GATA198B05等9个STR基因座的遗传多态性。方法用本实验室构建的9个常染色体STR基因座荧光复合扩增体系,对辽宁鞍山岫岩满族252个无关个体的DNA进行PCR扩增,3130型基因分析仪电泳检测扩增产物及等位基因分型标准物,GeneMapper誖3.2分析软件中导入本体系Panel和Bin,对电泳结果进行分析,按照重复序列重复次数命名等位基因,使用Power-StatsV12和GENEPOP软件进行统计学分析。结果 9个STR基因座在辽宁鞍山岫岩满族基因型频率分布均符合Hardy-Weinberg平衡(P﹥0.05),多态性信息含量在0.750～0.860之间,杂合度在0.794～0.881之间,个体识别力在0.918～0.968之间,非父排除概率在0.587～0.757之间,累积非父排除率为0.999 96,累积个体识别能力为0.999 999 999 999 1。结论这9个非CODIS系统STR基因座在鞍山岫岩满族群体中等...