跨境电子商务对进出口贸易影响的实证分析

该文采用理论模型与实证分析相结合的方法,研究了中国跨境电子商务发展对进出口贸易及贸易周期的影响。该文创新点在于：以不完全竞争市场为基础,建立电子商务环境下的国际贸易市场模型,同时运用实证分析,采用协整分析方法,进一步建立ARCH模型和向量自回归模型,以揭示跨境电子商务发展与进出口贸易的关系。研究表明,跨境电子商务发展与进出口贸易之间存在长期均衡关系,跨境电子商务的发展能够增加进出口贸易的波动性,并且呈同方向变化。     

高职院校“基础”课教学考核与评价方式改革研究

《思想道德修养与法律基础》作为高职院校思想政治理论课的入门课程,承担着对大学生进行思想政治教育的重任。目前,该课程在教学实践中存在着教学形式僵化、考核评价方式死板等问题。为改变这一现状,可以在推行课堂教学改革、落实课程实践环节的基础上,通过完善考核内容、调整分数比例、设置教学评价等级等方式,进行考核与评价方式的改革。     

Comparative Analysis of the HV1 and HV2 Regions of Human Mitochondrial DNA by Denaturing High-Performance Liquid Chromatography

Abstract:  Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a sequencing-independent means of detecting the presence of sequence differences in pair-wise mixtures of nonconcordant amplicons of human mitochondrial DNA (mtDNA). A total of 920 pair-wise combinations of HV1 and HV2 mtDNA amplicons from 95 individuals were assayed by DHPLC for sequence concordance/nonconcordance. For the 72 combinations of amplicons from different individuals who shared identical DNA sequences, DHPLC assays consistently indicated sequence concordance between the samples. This was in 100% agreement with sequencing data. For the 849 combinations of amplicons which differed in sequence, DHPLC detected the presence of sequence nonconcordance in all but 13 assays to yield 98.5% concordance with sequencing. Thus, DHPLC can be used to detect a diversity of sequence differences (transitions, transversions, insertions, and deletions) in the mtDNA D-loop. Accordingly, DHPLC may have utility as a presumptive indicator of mtDNA sequence concordance samples, as a screen for heteroplasmy/situational mixtures, and as a means for the physical fractionation of the individual contributors to an mtDNA mixture prior to sequencing.     

儿童精神分析学的主要方法及其应用

儿童精神分析的出现被视为是精神分析"新范式的开始".儿童精神分析学的方法主要来自并运用于临床实践与临床发现,迥异于学院心理学的方法,有其自身的特色与优势.其方法主要包括两个方面,一是研究方法,二是治疗方法.儿童精神分析学最主要的方法包括重构法、观察法、实验法、测验法以及游戏疗法.纵观儿童精神分析学的主要方法,我们会发现它们与发展心理学的研究方法有某些共同之处,但发展心理学则更多地是进行标准化研究和对发展过程的详细描绘,而儿童精神分析学者更多研究心理发展动力与潜意识的影响.二者在研究结果与研究内容方面出现了逐渐融合的趋势,越来越多的儿童精神分析学的研究主题,如母婴关系、自我功能、自我发展、分离与剥夺、受挫与攻击性等逐渐纳入发展心理学的视野.     

Comparison of modern techniques for saliva screening

Saliva stains present a unique challenge in the forensic setting, often challenging the analyst to weigh the value of presumptive indication of the fluid versus the potential for DNA analysis to yield identification information. There are many situations in which determining the presence of a body fluid is probative and further corroborates DNA evidence. That said, even a minute portion of sample consumed by a screening test could mean the difference between a full, partial, or null profile obtained through DNA analysis. The basis of presumptive testing or screening of saliva has historically been based on the presence of amylase, a component found in relatively high concentrations in human saliva versus other body fluids and substances. Though the current available methods for the screening of saliva in a forensic application have grown in number, the popularity of these methods seemingly has not. This study attempts to identify a specific and sensitive saliva screening test by comparing three modern techniques--the recently released SALIgAE, Phadebas, and starch-iodine mini-centrifuge test--on the basis of sensitivity, specificity, mixtures, and simulated casework samples while also considering sample consumption. The Phadebas method for presumptive saliva testing detected dilutions of neat saliva down to 1:200 versus considerably less sensitive results with SALIgAE and the starch-iodine mini-centrifuge test. Utilizing a screening test with a high degree of sensitivity, such as Phadebas, allows an analyst to gain a maximum amount of information in the form of body fluid indication and DNA results because of the consumption of a small portion of sample.     

美国反垄断法中界定“相关市场”的临界损失分析法

美国早期用来界定"相关市场"的方法没有限定市场弹性幅度,这个缺陷直到"假定的垄断者测试"产生后才得到弥补。1986年,美国改进了"假定的垄断者测试"操作方法,提出了临界损失分析法。虽然人们对临界损失分析法还存在不少争议,但它在美国和欧盟得到了越来越广泛的应用,相关判例得到了极大的丰富。     

Blinking Subjects; Blinking Justice? – Law,Medicine and the PVS Patient

This paper argues that in medical discourse, there is insufficient unanimity of opinion with regards to the time at which an accurate diagnosis of PVS can be made and that clearly, there is an incomplete medical knowledge of the PVS condition. The judiciary chooses neither to question medical opinion that patients can be considered to be in PVS despite a failure to satisfy the diagnostic criteria, nor medical opinion that patients in `near PVS' will never recover. It is apparent from an examination of the judgements given in PVS cases that the law does not ascribe such individuals with full human status. Such a legal position is particularly problematic in ethical terms when applied in cases involving patients who are in a `near PVS' position, and in the light of evidence that some PVS diagnoses are inaccurate. The application of the best interests test in PVS cases results in the adoption of a paternalistic, objective approach that fails to respect the former competent individuals whom PVS patients once were. If, alternatively, the substituted judgement test were to be adopted, the principle of individual autonomy would become central to the question of whether PVS patients' treatment should be withdrawn. Furthermore, the application of this test would also ensure that PVS patients continue to be viewed as `persons'.     

抗人血红蛋白胶体金检测试剂条的研制

目的制备法医学检验所用的确定人血的免疫胶体金层析试剂条。方法选取抗人血红蛋白单克隆细胞株,制备其小鼠腹水,从腹水中纯化出单克隆抗体。制备胶体金并用一纯化的单克隆抗体包被,制成免疫胶体金。取玻璃纤维以免疫胶体金浸泡,烘干。在一硝酸纤维素膜上两个不同位置分别点加另一抗人血红蛋白抗体和羊抗鼠IgG。搭建试剂条并检测其灵敏度和特异性。结果制成的免疫胶体金试剂条可对稀释至20万倍的人血红蛋白溶液显示阳性,对法医学检验常见8种动物的血溶液显示阴性。结论所制备抗人血红蛋白胶体金试剂条可以应用于法医学检验。     

鸽源禽毛滴虫的体外药敏试验

利用体外培养的禽毛滴虫对8种市面上常用药物进行了体外抗禽毛滴虫药敏试验。在发育繁殖稳定的禽毛滴虫的培养管中,分别加入不同种类及其不同浓度的受试药物,37℃作用2和6 h后,计算各管活虫数;并与不加药物的培养管中的活虫数比较,计算活虫率,以此为指标评价不同药物的杀虫效果。结果表明,二甲硝唑、替硝唑具有显著的杀毛滴虫作用,制霉菌素、环丙沙星、复方磺胺甲唑仅有部分抑制作用而不能将滴虫完全杀灭,泰乐菌素、复方甘草、盐酸小檗碱杀虫作用不明显。试验结果为临床选用抗禽毛滴虫药物提供了依据。     

燃气加气站地下储罐的置换和试压方法的探讨

介绍了汽车用燃气加气站地下储罐的置换和试压方法 ,对地下储罐系统试压方法的经济性进行了分析