福建公安高等专科学校男生心理健康素质状况测查报告

本研究以MMPI为工具 ,测查福建公安高等专科学校男生的心理健康素质状况 ,结果显示 ,该校男生心理健康素质状况喜忧参半 ,并有自己的特点。尤其值得重视的是 ,有心理病态嫌疑或心理不适倾向的个案占 63 .6 % ,其中“亚健康”者占 44 .5% ;疑似病态的偏常类型以躁狂倾向、病态人格、躁郁倾向为主 ,合计占病态个案的 81 .0 %。因此 ,公安院校招生和公安机关招警应考虑引进心理测试筛查心理健康偏常者 ;公安院校应加强在校学生的心理健康辅导和心理素质训练 ,建立心理健康保障体制。     

DNA指纹技术在亲子鉴定中的应用

用非放射性标记α－珠蛋白－３＇ＨＶＲ探针在低强度洗涤条件下，对人的ＤＮＡ指纹进行了分析，获得了清晰易读的ＤＮＡ指纹图。并用该方法调查了云南省的７８名无关个体，经统计学分析，计算出无关个体的相关机率是２．４×１０－４。用该方法进行亲子鉴定，获得了满意的结果，用ＨｉｎｆⅠ和ＨｅａⅢ两种内切酶酶切，解决了子代陌生带问题，用该方法对二十余起亲权纠纷案进行了鉴定，证明该方法稳定、结果可靠，父权肯定机率均在９９．９％以上。     

商务日语专业建设的探索

20世纪90年代以来,随着日本对中国投资的扩大,中国商务日语人才严重缺乏。在此背景下,不同层次高校纷纷设立商务日语专业（方向）,但是该专业目前的学科定位尚不明确,高校日语教师不熟悉商务活动,师资条件、教材内容、课程设置、培养模式等方面存在一系列问题。目前商务日语资格考试对商务日语专业的学科建设可以起导向作用,参照考试内容和能力标准可以不断探索本专业的课程设置、教学内容体系以及培养模式等。     

心理测试技术应用于交通事故驾驶人认定的思考

心理测试技术是一项通过测试人内心的心理痕迹,来探测人对某件事的感知和记忆的技术。目前在交通事故驾驶人认定中应用很少,本文拟通过研究心理测试技术在认定驾驶人身份中的应用,阐明该技术对于认定驾驶人身份的独特作用,以及应用该技术的前提条件、操作的步骤和要求等,为侦破疑难交通事故案件提供了一种有效的辅助手段。     

Validation studies of rapid stain identification-blood (RSID-blood) kit in forensic caseworks

When bloodstains are detected at crime scene using presumptive tests (e.g. luminol, phenolphthalein, leuchomalachite green), it is important to establish the real human nature of each stain. This is possible using confirmatory tests. One of these is rapid stain identification-blood (RISD-blood) a lateral flow immuno-chromatographic strip test format which allows the identification of human blood by detection of glycophorin A, a red blood cell membrane antigen, using two anti-human glycophorin A (GPA) monoclonal antibodies.The aim of this study is to assess the sensitivity of RSID-blood test in old, degraded bloodstains and in some bloodstains previously treated with BlueStar Forensic, a presumptive test which is often used in crime scene investigations to detect latent bloodstains. The genetic analysis of all bloodstains of confirmed human nature was subsequently performed using the AmpF1STR Identifiler PCR Amplification Kit (Applied Biosystems), to validate the possibility of obtain a consistent and reliable DNA typing results.     

领导干部的OSL品德测评模式及其运行机制——基于部分省市党政干部品德测评实践的思考

德才兼备以德为先,一直是我们国家的人才政治原则。本文在回顾国家相关文件政策与分析当前党政领导干部品德测评问题与实践的基础上,基于品德的资本性、可测量性与可开发性,从人力资源开发的视角,提出了包含"评价、任用、开发"的一体化的OSL动态测评模式,并对这种测评模式提出的背景与原因,内涵、结构及其运行机制进行了相关的分析。文章认为,品德测评不是目的,目的是要通过品德测评来提升领导干部的品德素养。本文设计的OSL测评模式,不仅仅在于对领导干部品德行为的养成效果进行评定,而且这种有组织有计划的品德行为测评本身模式具有开发的功效,能够对领导干部的行为养成进行反馈、调节与引领。     

互联网企业市场支配地位认定之思考

互联网市场有别于传统市场，特别是双边市场特征、“互联网效应”和“锁定效应”等，使得传统的相关市场界定和市场支配地位认定方法难以有效发挥作用。在宏观层面，应将互联网市场界定为采用互联网方式经营的虚拟产品市场；在微观层面，建议以“收益来源”为标准，配合“SSNIP测试法”对相关市场进行重新界定；在立法层面，应在《反垄断法》位阶下，出台针对互联网市场的指导性文件，同时，诉方仅应承担初步举证责任。     

The use of Hemastix® severely reduces DNA recovery using the BioRobot® EZ1

Abstract: The choice of reagents for presumptive tests for blood, and subsequent extraction methodologies, can significantly affect both the quantity and quality of purified DNA. Blood samples directly tested with Hemastix^® yielded <1% of the DNA recovered from untested samples when purified using the Qiagen BioRobot^® EZ1 and EZ1^® DNA Investigator kit. Full short tandem repeat profiles were obtained from both tested and untested samples, suggesting that the Hemastix^® reagent(s) affect DNA binding, rather than produce DNA damage. The Hemastix^® inhibition of DNA yield could be overcome by the addition of MTL buffer to the sample prior to extraction. Laboratories may wish to modify current procedures for extracting blood samples, utilize other extraction/purification methodologies, or inform their submitting agencies to avoid direct exposure of questioned bloodstains to Hemastix^® reagents.     

高致病性猪繁殖与呼吸综合征病毒HBR变异株第125代的毒力试验

从临床病例中分离到1株高致病性猪繁殖与呼吸综合征病毒(PRRSV)变异株HBR,经细胞传代和蚀斑克隆获得了1株增殖性能稳定的传代毒株。为了检测该毒株在Marc-145细胞传代过程中毒力的变化,用PRRSV-HBR株第125代培养物(毒价为107.5 TCID50/mL)经肌肉注射和滴鼻途径接种18头35日龄PRRSV抗体阴性猪,同时设未接种对照组。结果试验猪未出现明显的临床症状。用RT-PCR法检测试验猪血清,从第3天开始出现阳性,第7～14天达到高峰,第28天后转为阴性。对同一时间迫杀的试验猪检测各脏器组织中的病毒抗原分布情况,结果表明,感染后第7～14天试验猪多数脏器呈阳性反应,第21～42天病毒集中在脾、腹股沟淋巴结、颌下淋巴结等组织。病理学观察发现,第7～14天试验猪淋巴结及肺出现轻度病变。动物感染试验表明,尽管试验猪未显示明显的临床症状,但从病毒血症、病毒体内分布、病理变化等方面看,该毒株仍然具有一定的毒力,用于弱毒疫苗的研制还有待进一步驯化致弱。     

Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs

Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas^® Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5–32 μL) with high or intermediate amylase activity (840 and 290 kU/L). Results were typically obtained within 5 min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even so, a positive amylase result indicates presence of saliva, and thereby presence of DNA. Amylase testing may be useful for screening in investigations where the number of DNA analyses is limited due to cost, e.g., in volume crime.