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61.
We examine an experimental‐design reemployment program implemented in Nevada during the Great Recession that required Unemployment Insurance (UI) recipients to: (1) undergo an eligibility review to confirm they were qualified for benefits and actively searching for work and, if deemed eligible, (2) receive job‐counseling services. Our results show that the program expedited participant exit from UI, produced UI savings that exceeded program costs, and improved participant employment outcomes. Analyses of program effects on the UI exit likelihood show that the program's effects are partly associated with increased participant exit up through the time when program activities were scheduled, reflecting voluntary exit of participants from UI to avoid program activities and disqualifications of participants who failed to meet eligibility requirements. In addition, the program induced substantial participant exit from UI in the period after participants fulfilled requirements and their interactions with the program had ended, suggesting that the job‐counseling services offered by the program may have helped participants to conduct more effective job searches. Our findings provide evidence that reemployment programs that combine an eligibility review with mandatory participation in job‐search services can be effective during recessions. 相似文献
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H3亚型猪流感病毒血凝素基因在昆虫细胞中的表达及其间接ELISA检测方法的建立 总被引:1,自引:0,他引:1
根据GenBank上登录的H3亚型猪流感病毒HA基因序列设计了1对引物,经PCR扩增出HA基因目的片段,并将其克隆到pFastBacGP67C杆状病毒载体上,筛选阳性重组转座载体pFastBacGP67C-H3,转化至含有杆状病毒穿梭载体(bacmid)的DH10Bac感受态细胞,构建杆状病毒表达载体,获得重组转座子rBacmid-H3,在脂质体介导下转染处于对数生长期的sf9昆虫细胞,获得重组杆状病毒rBv-H3,将其感染sf9昆虫细胞,收获目的蛋白。经血凝试验、SDS-PAGE、Western-blot、免疫组织化学分析,结果表明,HA蛋白得到表达,且具有良好的免疫学活性。用表达的HA蛋白作为包被抗原,初步建立了H3亚型猪流感病毒的间接ELISA检测方法,通过对93份送检的疑似猪流感血清进行检测,结果显示,检出阳性率为46.24%。上述结果为建立快速、准确、简便的H3亚型猪流感鉴别诊断试剂盒奠定了基础。 相似文献
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In this note, we use data from different elections in the German state of North-Rhine Westphalia between 1975 and 2010 to show that the Conservatives profit from lower voter turnout at the expense of the Social Democrats. We deal with the endogeneity of voter turnout by using election day rain as an instrumental variable. Our particular contribution is the comparison of municipal and state elections. 相似文献
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《Journal of Intervention and Statebuilding》2013,7(5):545-555
ABSTRACTTwenty years of international statebuilding have made little impact on the ‘stateness’ of Kosovo. This article puts this failure in the broader context of the shift from the liberal internationalist assumptions of the late 1990s to the pragmatic realism of today. It does this through the lenses of E H Carr’s classic work The Twenty Years’ Crisis, UN policy thinking on the problematic assumptions of international statebuilding and the diagnoses in David Lake’s 2016 book The Statebuilder’s Dilemma, which sets out three pragmatic alternatives. The article concludes that all three of these alternatives can be seen in practice in Kosovo. 相似文献
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根据已发表的H5N1禽流感病毒NS基因全序列设计了1对引物,对4株禽流感病毒新疆株的NS基因进行了RT-PCR扩增,并将其克隆到pMD 18-T载体上,分别获得了全长为817、851、8548、54 bp的全基因序列。序列分析结果表明,新疆毒株间的核苷酸同源性为97.8%~98.9%,氨基酸同源性为96.5%~98.7%,与广东、香港和东南亚等不同地区的11个毒株比较,同源性为90.0%~99.4%;与来自野禽、猪等不同种类的11个流感毒株比较,同源性为81.4%~99.3%。系统进化树分析表明,新疆株独立分支。 相似文献
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从pMD18-T-HA阳性质粒中扩增出H7亚型禽流感病毒(AIV)HA1基因,并亚克隆至昆虫杆状病毒转移载体pBlueBacHis2A,将筛选的重组质粒命名为pBlueBacHisH7-HA1,与线性化杆状病毒DNA(Bac-N-BlueDNA)共转染sf9昆虫细胞,挑取蓝色蚀斑,经3次蚀斑纯化,得到重组杆状病毒rpBlueHisH7HA1。Western-blotting和间接免疫荧光试验结果显示,HA1在昆虫细胞中得到表达,且表达产物具有抗原性。抗原特异性试验证明,重组HA1蛋白与鸡H5、H9亚型AIV抗血清不发生交叉反应。血凝试验证明,重组HA1蛋白具有良好的血凝性。 相似文献