乙肝Ⅲ号合近红外信息辐照治疗乙型肝炎肝纤维化的临床研究

目的：观察乙肝Ⅲ号合近红外信息辐照治疗乙型肝炎肝纤维化的疗效。方法：选择乙型肝炎肝纤维化住院病人234例，分为乙肝Ⅲ号治疗组(76例)、近红外信息辐照组(73例)和联合治疗组(85例)。在基础治疗(包括甘利欣，复方丹参液等)上采用单纯的中药外敷、肝病治疗仪近红外信息辐照以及两联合治疗等三种不同方法，观察各组患临床症状、体征、肝功能以及相关指标如PGA、肝纤三项(透明质酸(HA)、层黏蛋白(LN)、CⅣ型胶原(CⅣ))、肝血流的变化。结果：3组皆能改善临床症状，但以联合治疗组为；3组肝功能复常比较，差异有显性，但组间比较，差异无显性；在改善PGA、肝纤三项和肝血流量方面，联合治疗组优于乙肝Ⅲ号治疗组和近红外信息辐照组。结论：乙肝Ⅲ号合近红外信息辐照对乙型肝炎肝纤维化有一定的治疗作用，且较单一方法疗效明显。     

保护规范理论的始源形态——布勒公权论的再认识

布勒的公权理论可谓保护规范理论的始源形态,应当立足于其历史脉络与整体结构来理解。在布勒的保护规范理论中,法规的强行性排除了裁量规范,但包含不确定法律概念的法规范仍具有强行性。私益保护目的以公私益的划分为基础,构成了布勒保护规范理论的核心要义。援用可能性不同于诉讼可能性,是指受保护的私人可以援用法规范请求行政机关为特定行为。只要法律并未明确排除,即可承认援用可能性的存在。经由布勒保护规范理论所得出的公权具有严格的法规从属性,但也使公权受立法者的宰制。此外,布勒所讨论的公权仅指以特定实体决定为内容的实体性公权,程序性公权被排除在外。在审判实践中,我国法院应对布勒的旧保护规范理论保持警觉,合理地确定行政诉讼的原告资格。     

改善油田基层乙方员工心理健康的对策分析

作为国有企业,油田肩负着发展中国石油事业的重大责任和使命。然而,油田基层长期奋斗一线的乙方员工因工作性质和工作环境问题,频繁出现紧张、悲观、麻痹、浮躁和不合作等五种不健康心理,对此,要结合管理者、思想政治工作者、心理医生和员工自身四方面的努力,才能逐步改善油田基层乙方员工的心理健康问题。     

金黄色葡萄球菌黏附素FnBPB-ClFA共表达质粒的构建与原核表达

采用PCR方法对编码金黄色葡萄球菌纤黏蛋白B的D区和纤黏蛋白原凝集素A的A区基因片段进行了特异性扩增,并通过重叠延伸PCR扩增了FnBPB-ClFA串联基因,构建了克隆质粒pMD19-FnB-PB-ClFA。将该基因片段定向插入到原核表达载体pET-32a(+)中,构建了表达质粒pET-FnBPB-ClFA,并将其转入宿主菌BL21(DE3)。SDS-PAGE分析表明,在1mmol/L IPTG诱导下,在51ku处出现了与目的蛋白一致的外源蛋白带。Western-blot分析表明,所表达的蛋白与目的蛋白一致。上述结果表明,该融合基因在原核细胞中成功表达。     

马立克氏病病毒gB基因的克隆及重组鸡痘病毒的构建

通过PCR方法扩增了马立克氏病病毒 (MDV) 2 .8kbgB基因 ,并且在其两端加上PstⅠ和NotⅠ酶切位点。然后用PstⅠ /NotⅠ酶切回收 gB片段 ,插入到转移载体 pEFgpt12s复合启动子Spromoter的下游的PstⅠ /NotⅠ酶切位点处 ,构建了转移质粒pEFgpt12s gB。将该质粒与鸡痘病毒 (FPV)野毒 2 82E4株共转染鸡胚成纤维细胞 (CEF) ,通过MXHAT选择培养基进行筛选 ,蚀斑纯化 ,经PCR扩增证实重组病毒中含有 gB基因。重组病毒和FPV野毒在CEF上形成空斑大小无明显差别 ,病毒效价分别为 10 -5.43 TCID50 /mL和 10 -6TCID50 /mL ,表明外源基因的插入不影响所构建的重组病毒的生物学特性     

金水宝合拉米夫定治疗慢性乙型肝炎60例

目的：观察中药金水宝、水林佳合拉米夫定治疗慢性乙型病毒性肝炎的效果。方法：将89例慢性乙型肝炎患随机分成治疗组60例，对照组29例。疗程为9个月至2年。结果：治疗9个月后，治疗组在HBsAg、HBeAg、HBV-DNA阴转率，肝功能复常率及症状体征的恢复率方面优于对照组。结论：中西医结合治疗慢性乙型肝炎，能抑制病毒复制，改善肝脏循环和肝功能，提高疗效，缩短疗程。     

核因子-кB及其在创伤性脑损伤后的作用

核因子-кB是一种具有多向性调节作用的核转录因子。机体受到创伤后,NF-кB被激活,调节多种炎性介质基因转录,引起局部及全身的炎症反应。对NF-кB的有关研究可望用于法医学家对损伤时间的推测。     

Kenneth B. Clark and the problem of power

ABSTRACT

During the early 1960s African American psychologist Kenneth B. Clark, known primarily for his involvement in the 1954 Brown v. Board of Education US Supreme Court desegregation decision, began organizing an ambitious anti-poverty programme called Harlem Youth Opportunities Unlimited, Inc. (HARYOU). Dissatisfied by the lack of progress in school desegregation in New York City and discouraged by the inability of traditional social welfare organizations to address the problems of race and poverty, Clark argued that a new approach had to be developed to mobilize the black poor to gain the political and economic power that would solve their problems. At the same time, he theorized that a new form of racial segregation was beginning to develop in urban areas that foreshadowed increasing social isolation, economic dependence and declining municipal services for many African Americans. He called this new development ‘internal colonialism’ and hoped that HARYOU would be a demonstration project in the Kennedy–Johnson administration's War on Poverty that would address these problems from multiple perspectives. Nonetheless, the plan aroused the political opposition of Harlem Congressman Adam Clayton Powell. The dispute with Powell drove Clark from HARYOU and caused him to re-evaluate his thinking regarding African American leadership. He increasingly viewed the ‘ghetto’ as both a prison and a cocoon that satisfied white and black social, economic, political and psychological needs. By the end of his HARYOU experience, Clark coined the term ‘the new American dilemma’ to describe and theorize about an increasingly isolated and powerless black population in many urban centres. The term also signified his belief that the problem of power was intricately tied up in, while also separate from, the problem of race.     

Death Due to Complications of Anhidrotic Ectodermal Dysplasia

Ectodermal dysplasia comprises a group of disorders affecting ectodermal tissues. Severity depends on the genetic aberration; hyperpyrexia secondary to absence of sweat glands is a common complication. Treatment is supportive. This case report describes a 1‐month, 27‐day‐old male infant with a diagnosis of X‐linked recessive anhidrotic ectodermal dysplasia. On the day of his death, his mother swaddled him in a blanket and placed him on the couch at 5:30 am. When she picked him up at 8:00 am, he was unresponsive. At the emergency department, his rectal temperature was 40°C. Postmortem blood culture was positive for group B streptococcus, a possible etiology for fever. It is vital to teach parents that close monitoring of children with ectodermal dysplasia is necessary, as an increase in body temperature can become life threatening.