口蹄疫病毒A/WH/09株结构蛋白P1基因的克隆及其B细胞抗原表位的预测

对口蹄疫病毒(FMDV)A/WH/09株P1结构蛋白基因进行了扩增、克隆及序列测定。结果显示,FMDV A/WH/09株P1基因长2 208bp,包含完整的开放阅读框,编码736个氨基酸,其中VP1长636bp,编码212个氨基酸;VP2长654bp,编码218个氨基酸;VP3长663bp,编码221个氨基酸;VP4长255bp,编码85个氨基酸。采用DNAStar Protean软件对P1蛋白的二级结构、可塑性、亲水性、表面可及性及抗原指数等参数进行了综合分析,并预测其B细胞的抗原表位分布。结果表明,VP1、VP2和VP3上均有多个区域为B细胞抗原优势表位,VP4上也有少量潜在的B细胞抗原表位。与已鉴定的B细胞抗原表位相比,本试验所用方法预测的结果有较高的准确度,是一种较为先进的表位研究方法。     

Sudden Death Secondary to an Undiagnosed B‐Cell Lymphoma of the Hypopharynx and Infiltration of the Inferior Constrictor Muscle

The aim of this presentation was to share an uncommon form of sudden death, suffered by a 64‐year‐old woman, due to a mechanical obstruction of hypopharynx by an undiagnosed B‐cell lymphoma, infiltrating the inferior pharyngeal constrictor muscle. A forensic approach by means of scene investigation, circumstantial data collection, autopsy, and histological and toxicological investigations led to conclude that the cause of death was asphyxia, correlated with B‐cell lymphoma of the hypopharynx. The autopsy examination highlighted the presence of a wall thickening, infiltrating, and projecting into the hypopharynx lumen. The histological analysis showed the essential finding of a B‐cell lymphoma of the hypopharynx, diffusely infiltrating the inferior pharyngeal constrictor muscle. To conclude, this case demonstrates once more that in the absence of specific data, a thorough forensic investigation including autopsy, histological examination, and circumstantial data collection is mandatory to reach a correct cause of death.     

The Upper Silesia (Poland) population and forensic usefulness of 11 autosomal STR loci

Allele frequencies, forensic parameters for the 11 STR loci in GenePrint STR Systems—Silver Stain Detection (Promega): CSF1PO, TPOX, TH01, D16S539, D7S820, D13S317, F13A01, FESFPS, VWA, F13B, LPL were determined in a sample of 529–1436 unrelated, dead and alive adults from the Upper Silesia region (Poland). Furthermore, gender identification was carried out. The values of heterozygosity (Ht), polymorphic information content (PIC), power of discrimination (PD), matching probability (PM), mean exclusion chance (MEC) and mean exclusion probability (MEP) were calculated using Dudek's computer programme (FatRec, High Technical School of Cz?stochowa, Poland). A possible divergence from HWE was determined by χ² and exact tests using Miller's computer programme (TFPGA, Northern Arizona University, Flagstaff, USA). Comparison of allele frequencies between the Upper Silesia population and other Polish populations was performed with Carmody's test.     

Developmental validation of a Cannabis sativa STR multiplex system for forensic analysis

A developmental validation study based on recommendations of the Scientific Working Group on DNA Analysis Methods (SWGDAM) was conducted on a multiplex system of 10 Cannabis sativa short tandem repeat loci. Amplification of the loci in four multiplex reactions was tested across DNA from dried root, stem, and leaf sources, and DNA from fresh, frozen, and dried leaf tissue with a template DNA range of 10.0-0.01 ng. The loci were amplified and scored consistently for all DNA sources when DNA template was in the range of 10.0-1.0 ng. Some allelic dropout and PCR failure occurred in reactions with lower template DNA amounts. Overall, amplification was best using 10.0 ng of template DNA from dried leaf tissue indicating that this is the optimal source material. Cross species amplification was observed in Humulus lupulus for three loci but there was no allelic overlap. This is the first study following SWGDAM validation guidelines to validate short tandem repeat markers for forensic use in plants.     

C/S、B/S结构相结合的公安院校远程招生系统设计

本文提出了建立基于C/S、B/S体系结构相结合的公安院校远程招生系统,给出了总体设计方案,包括系统结构、系统模块功能和数据库表结构,提出了系统设计的原则,介绍了系统所采用的软件平台和所使用的基本技术。     

提单与票据之比较研究

有关提单的法律规定尚不完整 ,因提单和票据实质上有许多相似之处 ,人们可以利用票据法的一些规定来解决提单的一些问题。本文比较研究了提单和票据的法律性质以及二者在转让中的前后手权利优劣等事项。     

B. F. Skinner's utopian vision: behind and beyond walden two

This paper addresses B. F. Skinner's utopian vision for enhancing social justice and human well‐being as it was introduced in his 1948 novel, Walden Two. In the first part, we address the historical, intellectual, and social context that situates the book in the utopian genre, the critiques of its premises and practices, and the fate of intentional communities patterned on the book. In the second part, we review practices in Skinner's book that advance social justice and human well‐being under the themes of health, wealth, and wisdom, and then focus on contemporary practices that are the legacy of his vision. His vision was neither the a priori truth of a particular premise about human behavior or a necessary practice (blueprint) for an intentional community but rather the use of empirical methods to discover premises and practices that work to advance the health, wealth, and wisdom of individuals and survival of the culture.     

公安管理信息系统的设计与实现

随着计算机网络的发展和公安工作的扩大，原有的公安软件已不能满足现实的需要．在此基础上，本论文对公安业务进行分析，提出了用C／S和B／S混合使用的模式来开发和实现公安信息管理系统，并对未来的发展作了展望。     

鸡盲肠扁桃体中B淋巴细胞的发育

通过免疫组织化学方法,应用IgM、IgG和IgA单克隆抗体研究了IgM~+、IgG~+和IgA~+B淋巴细胞在鸡盲肠扁桃体中的出现、迁移、组织定位分布以及数量变化规律等一系列发育过程.结果显示,IgM~+细胞在胚胎20日龄时开始出现,而IgG~+和IgA~+细胞均在雏鸡出壳后1日龄时出现.在定位分布变化上,4日龄之前,B淋巴细胞主要分布在黏膜上皮下固有层中;4～7日龄时,B淋巴细胞则主要分布在黏膜固有层的中上部区域,随后各日龄B淋巴细胞均匀地分布在黏膜固有层中;14日龄时,黏膜固有层中出现主要由B淋巴细胞组成的生发中心,21日龄时可见轮廓更清晰的生发中心存在于黏膜固有层中下部区域,并且生发中心中存在大量的IgM~+、IgG~+和IgA~+细胞.在数量变化上,B淋巴细胞随着日龄增长数量持续增加,直至35日龄时趋于稳定,各日龄的B淋巴细胞数量始终以IgM~+细胞最多,IgA~+细胞次之,IgG~+细胞最少.证实,鸡出壳后初期盲肠扁桃体的体液免疫功能逐渐增强,并在35日龄时达到成熟水平.