Multiplex high resolution melt (HRM) messenger RNA profiling assays for body fluid identification

Traditional body fluid identification methods use a variety of technologically diverse techniques that do not permit the identification of all body fluids. Definitive identification of the biological material present can be crucial to a fuller understanding of the circumstances pertaining to a crime. Thus definitive molecular based strategies for the conclusive identification of forensically relevant biological fluids need to be developed. Messenger (mRNA) profiling is an example of such a molecular based approach.Current mRNA body fluid identification assays typically involve either capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the time required for analysis. For qRT-PCR assays, only 3 or 4 markers can be included in a single reaction since each requires a different fluorescent dye. To simplify mRNA profiling assays and to reduce the time and cost of analysis, we have developed multiplex high resolution melt (HRM) assays that provide an identification of all forensically relevant biological fluids and tissues.     

Explaining Taiwan's policies in the South China Sea, 1988–99

The current paper discusses Taiwan's policies in the South China Sea during the period 1988–99. These policies are discussed with reference to ‘realist’ and ‘non-realist’ theoretical approaches. The realist position regards Taiwan's South China Sea policies as an outcome of its relations with the People's Republic of China and the Southeast Asian countries. These policies are fashioned and implemented in a coherent way by a unitary state. Two ‘non-realist’ positions are identified. One focuses on influences from domestic political parties and party factions, bureaucratic segments and economic interests. The other emphasizes the impact of transnational alliances, mainly through oil business lobby groups allied with mainland Chinese partners. The investigation sustains much of the realist argument. It is, however, argued that party politics and bureaucratic infighting has had an independent effect on Taiwan's South China Sea policies, while the impact of oil business interests has been limited.     

The interconnections of political power,psychotherapy, and world community

This study of the main evening television news programs in four European countries focuses on the framing of news surrounding a major European event, the January 1, 1999, introduction of the common European currency, the euro. We investigated the visibility of political and economic news in general and of the launch of the euro in particular. We found variations across countries in the emphasis on political and economic news, with the proportion of the newscast normally devoted to these subjects ranging from 45% to 60%. Journalists in all countries were more likely to emphasize conflict (rather than economic consequences) in framing general political and economic news. In the coverage of the launch of the euro, there was a greater emphasis on framing the news in terms of economic consequences. The findings are discussed in terms of influences on framing practices internal and external to journalism and the value of the cross-national comparative approach.     

Problems of the press in Latin America

This article examines whether the inauguration of peace between countries has a significant effect on how the news media cover the other side. It is argued that, due to the nature of news, leaders will generally find it easier to mobilize the media for conflict than for peace. However, the actual role the media will play in such attempts can be understood by looking at the political and media environments in which journalists construct news about peace. A joint project was conducted involving both Israeli and Jordanian researchers. The methodology included in-depth interviews with journalists from both countries and a content analysis of newspaper articles published during three different historical periods. The findings demonstrate that although there was a temporary improvement in the media image of the other side, there was little evidence that peace had a significant and lasting influence on coverage. There were, however, some important changes in the prominence of certain news slots. The interviews with the journalists provided valuable insights about some of the political and professional reasons for these findings.     

Civil society and trust building in Cyprus

Civil society in Cyprus has a key role to play in creating spaces for dialogue and cooperation between the Turkish and Greek Cypriot communities. Recent research by the International NGO Training and Research Centre (INTRAC) has identified how these processes have contributed to trust and peacebuilding on the island, the challenges facing organisations engaged in this work, and how these were being addressed. It demonstrates that important steps are being taken by civil society organisations to overcome prejudices and break down barriers, and that by further developing links with local and international policymakers and institutions, civil society could be a stronger player in the peace process.

Renforcement de la société civile et de la confiance à Chypre

La société civile chypriote a un rôle clé à jouer dans la création d'espaces propices au dialogue et à la coopération entre les communautés turque et gréco-chypriote. Des recherches récemment menées par l'International Training and Research Centre (INTRAC) ont identifié la manière dont ces processus ont contribué au renforcement de la confiance et de la paix sur l'île, les défis auxquels sont confrontées les organisations actives dans ce travail, et la manière dont ces défis étaient relevés. Les résultats de ces recherches montrent que des mesures importantes sont prises par les organisations de la société civile pour venir à bout des préjugés et éradiquer les barrières, et qu'en mettant au point des liens avec des décideurs et des institutions internationaux, la société civile pourrait être un protagoniste plus puissant dans le processus de paix.

La sociedad civil y la construcción de confianza en Chipre

En Chipre, la sociedad civil desempeña un rol importante en la creación de espacios para el diálogo y en la cooperación entre las comunidades turca y griega chipriota. Investigaciones recientes, realizadas por el Centro Internacional para la Capacitación y la Investigación (intrac por sus siglas en inglés), han identificado cómo estos procesos han aportado confianza logrando construir paz en la isla, cuáles son los retos enfrentados por las organizaciones que participan en este trabajo, y cómo fueron abordados dichos retos. Asimismo, dichas investigaciones demuestran que las organizaciones de la sociedad civil han dado pasos importantes para superar prejuicios y barreras y que, al desarrollar relaciones adicionales con los formadores de políticas y con las instituciones, la sociedad civil podría convertirse en un actor más fuerte en el proceso de paz.

Sociedade civil e construção da confiança em Chipre

A sociedade civil em Chipre possui um papel-chave na criação de espaços para diálogo e cooperação entre as comunidades cipriotas turcas e gregas. Pesquisa recente do Centro de Treinamento e Pesquisa Internacional (INTRAC) identificou como estes processos têm contribuído para a construção da confiança e paz na ilha, os desafios que as organizações enfrentam neste trabalho e como eles estão sendo abordados. Ela demonstra que passos importantes estão sendo dados pelas organizações da sociedade civil para superar preconceitos e destruir barreiras e, ao desenvolver mais as ligações com formuladores de políticas locais e internacionais e com instituições, a sociedade civil pode ser um agente mais forte no processo de paz.     

The success of Afghan NGOs

During the 1990s, and as part of a broader drive towards the “Afghanisation” of humanitarian assistance in Pakistan, Afghan NGOs became key implementing agencies supporting refugee-related and reconstruction activities in Afghanistan. This paper provides a detailed account of their emergence and consolidation, with a twofold objective. First, it complements and contributes to recent studies on Afghan NGOs and Afghan civil society, by providing historical insights on their trajectory. Second, it assesses such trajectory as a way of engaging with broader discussions on the role of NGOs within humanitarian operations.

Le succès des ONG afghanes

Durant les années 1990, et dans le cadre d'une initiative plus générale vers l’« afghanisation » de l'assistance humanitaire au Pakistan, les ONG afghanes sont devenues des agences clés de mise en ?uvre soutenant des activités de reconstruction et d'aide aux réfugiés en Afghanistan. Cet article propose un compte rendu détaillé de leur apparition et de leur consolidation, dans un double objectif. En premier lieu il complète et contribue à des études récentes sur les ONG afghanes et la société civile afghane, en donnant un aperçu historique de leur trajectoire. En second lieu, il évalue cette trajectoire dans le but de prendre part à des discussions plus larges sur le rôle des ONG dans les opérations humanitaires.

El éxito de las ong afganas

Como parte de un esfuerzo dirigido a “afganizar” la asistencia humanitaria en Pakistán durante los años noventa, las ong afganas se volvieron agencias operativas clave para el apoyo de actividades vinculadas a los refugiados y a la reconstrucción en Afganistán. El presente artículo proporciona una historia detallada de su aparición y de su consolidación y tiene dos objetivos. En primer lugar, complementar y contribuir a los estudios recientes respecto a las ong afganas y a la sociedad civil afgana, esclareciendo su trayectoria histórica. En segundo lugar, realizar una valoración de dicha trayectoria como una manera de participar en el debate más amplio en torno al rol jugado por las ong en el ámbito de las operaciones humanitarias.

O sucesso de ONGs afegãs

Durante a década de 1990, como parte de uma orientação mais geral para a “Afeganização” da assistência humanitária no Paquistão, ONGs afegãs tornaram-se agências implementadoras cruciais que apoiam atividades relacionadas a refugiados e à reconstrução no Afeganistão. Este artigo oferece um relato detalhado sobre o seu surgimento e consolidação, com um duplo objetivo. Primeiramente, ele complementa e contribui para estudos recentes sobre ONGs afegãs e a sociedade civil afegã, fornecendo informações históricas sobre sua trajetória. Em segundo lugar, ele avalia tal trajetória como forma de engajamento com discussões mais gerais sobre o papel de ONGs dentro de operações humanitárias.     

Dynamics of multi-local gifts: practices of humanitarian giving in post-tsunami Sri Lanka

This paper explores the paradox of gift giving in privately initiated forms of post-disaster aid. The paradox emerges from the gap that arises between ideals of the altruistic gift, and its practices in the actual implementation in a complex and multi-local humanitarian aid arena. An ethnographic study of a privately initiated post-tsunami housing project illustrates the paradox. While initiators presented the project as opposite to the mundane world of development aid, they increasingly came under pressure to deliver and perform visible success, such that their practices resembled this mundane world of humanitarian aid and its logics of patronage, favouritism, and politics.

La dynamique de dons multi-locaux: la pratique de donation humanitaire au Sri Lanka d'après-tsunami

Cet article étudie le paradoxe de donations sous des formes d'assistance d'après-désastre lancées par des organisations privées. Ce paradoxe est engendré par le vide qui s'ouvre entre l'idéal du don altruiste et sa pratique d'implantation dans l'arène d'aide humanitaire à la fois complexe et multi-locale. Il est illustré par une étude ethnographique d'un projet de logement mis en place par une organisation privée. Tandis que ceux qui ont lancé ce projet l'ont présenté comme le contraire du monde banal de l'aide développementale, ils ont de plus en plus subi une pression pour qu'ils livrent et finalisent une réussite visible, cela à un tel point que leur pratique a fini par ressembler à ce monde banal d'aide humanitaire et à sa logique du mécénat, du favoritisme et de la politique.

La dinámica de los regalos multi-locales: prácticas de donaciones humanitarias en el Sri Lanka de la fase post-tsunami

El presente artículo examina la paradoja que encierra el hecho de que particulares entreguen regalos como forma de ayuda post-desastre. La paradoja surge como consecuencia de la brecha creada entre los ideales que conlleva el regalo altruista y el modo en que son implementadas las prácticas en un ámbito de ayuda humanitaria complejo y multi-local. Para ilustrar dicha paradoja, el presente artículo se apoya en un estudio etnográfico realizado en torno a un proyecto de vivienda impulsado por particulares durante la fase post-tsunami. Quienes iniciaron este proyecto lo presentaron en contraposición al ámbito mundano de la ayuda de desarrollo. Sin embargo, se vieron cada vez más presionados a tener que demostrar éxitos palpables, al punto de que sus prácticas terminaron pareciéndose a las del ámbito mundano de la ayuda humanitaria y a su lógica de clientelismo, de favoritismo y de politiquería.

Dinâmica de mercadorias de vários locais: práticas de doações humanitárias no Sri Lanka após o tsunami

Este artigo explora o paradoxo das doações de mercadorias nas formas de ajuda iniciadas privadamente no pós-desastre. O paradoxo surge da distância que é criada entre os ideais da doação altruísta e suas práticas na implementação real em uma arena de ajuda humanitária complexa e multi-local. Um estudo etnográfico de um projeto de moradia pós-tsunami iniciado privadamente ilustra o paradoxo. Embora os iniciadores apresentaram o projeto como sendo oposto ao mundo mundano de ajuda ao desenvolvimento, eles foram cada vez mais pressionados para implementar e desempenhar iniciativas de sucesso visível, de forma que suas práticas parecessem com este mundo mundano de ajuda humanitária e sua lógica clientelista, de favoritismo e política.     

公海保护区的国际法基本原则辨析

目前,国际社会对公海保护区进行了一定程度的规范,但依然存在着没有直接的法律依据的处境。在此背景下,现有相关公约、条约或宣言等体现出来的法律原则为公海保护区提供了主要法理基础。公海保护区包括不同类别的法律原则,即国际法基本原则中直接与公海保护区有关的原则,国际环境法基本原则以及国际海洋法基本原则。公海保护区是适用于基本原则的领域,同时,它也是基本原则引中和发展出来的具体规则。     

美国涉外证券民事诉讼管辖权制度研究

文章以美国联邦证券法律体系中的涉外证券民事诉讼管辖权制度为对象,从管辖权的“法律基础”与“合理依据”两方面着手,重点论述了联邦证券法下的级别管辖、一般管辖问题。同时,对美国1971年《第二次冲突法重述》的相关规定及其在实践中遇到的问题进行了一定探讨。文章认为,对涉外证券立法严重滞后于相关实践的我国而言,美国在涉外证券管辖权方面的立法与实践,对我国相关制度的构建具有一定的借鉴意义。