流动注射—化学方光法检测人体血清内枸橼酸芬太尼

目的建立灵敏测定生物样品中皮克级枸橼酸芬太尼的方法。方法使用流动注射-化学发光法、鲁米诺-溶解氧体系检测芬太尼,蠕动泵流速2.0mL/min,鲁米诺与NaOH混合后被六通阀定量注入系统直至形成稳定基线,同时将样品推进送入混合管。碱性混合液被送入化学发光流通池产生化学发光,光电倍增管和光度计即检测到发光信号强度。结果在1.0～700pg/mL线性范围内,化学发光强度与芬太尼浓度呈线性关系,且发光强度随浓度增大呈增敏趋势,检出限低至0.3pg/mL。相对标准偏差在3.0%以内。分析过程可在30s内完成,采样频率120次/h。回收率98.9%～103.1%。结论该方法已经成功应用于检测人体血清中的枸橼酸芬太尼。     

农民工流动动因及特点研究综述

"流动性"既是农民工存在形式上的一大特点,也是当前解决农民工就业、教育等问题的症结所在。研究农民工的"乡-城"流动对认识农民工群体的形成、以及预测农民工群体的发展势态具有重要意义。     

检察机关案件流程管理模式的若干思考

实现检察案件管理模式的现代化,要分析研究案件质量管理自身的特点以及与全面质量管理模式间的趋同性,准确地寻找两者的结合点。科学构建检察案件管理模式的目标是实现检察化,要吸收现代质量管理模式的精华,又符合检察规律;科学的管理模式要解决成本大、程序繁琐的问题,以增强案件流程管理模式的操作性和实用性。     

Aggression, psychopathy and brain imaging — Review and future recommendations

Violent behavior appears to result from a complex web of interacting genetic as well as environmental factors. Psychopathy is a strong predictor for relapse in violent acts. The current review shed light on rapidly expanding knowledge in brain imaging related to violent behavior and psychopathy. A literature search was performed in PubMed, Cochrane and PsycInfo combining the key words: mentally disordered offender/aggression/violence/ crime/forensic psychiatry/brain imaging neuroimaging/fMRI/MRI/PET/SPECT/lack of empathy/psychopathy and antisocial personality disorder. The reviewed material, which consisted of 48 articles, indicates a rather strong consensus on the connection between dysfunctional parts of the frontal and temporal lobes and violent antisocial behavior and psychopathy. In future studies, it would be useful to focus on the limbic system and to investigate which parts of the frontal lobes and cerebral networks that are of interest in the psychopathic personality. Moreover, the reviewed material highlights some of the methodological difficulties in this area of research such as selection bias in the recruitment of patients, inadequate matching of control subjects, and sometimes incongruous results. In the future we hope that brain imaging can be used to map biological deviations in different offenders in order to try to learn more about the different mechanisms behind violent behaviors.     

死后组织DNA变化与死亡时间关系的研究

为调查死后组织DNA含量变化与死亡时间的关系,本文采用流式细胞仪对死后0～96小时(0～96hourpostmortem,hpm)大鼠的骨骼肌、脾、肾细胞DNA含量进行分析,结果表明,各组织显示了随死后间隔时间延长,含碎片DNA细胞增多,含完整DNA细胞减少的趋势;DNA降解系列组方图分析结果表明,脾组织降解速度明显快于肾和骨骼肌组织;骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解.     

东盟跨境数据流动治理的机制构建

跨境数据流动是全球数字经济的重要基础,也是全球治理的新兴议题。在数字经济发展和东盟区域一体化的趋势下,东盟从统筹数字经济发展与数据保护入手,着眼于提升东盟国际竞争力和话语权,制定并完善跨境数据流动治理机制。这一机制的构建始于新加坡对数据保护规制的探索,基于东盟个人数据保护框架,在东盟数字一体化总体框架下制定数字管理框架,并以“东盟示范合同条款”和“东盟跨境数据流动认证”两大关键手段促进机制落实。该机制具有灵活性、包容性和指导性的特征,有利于促进东盟地区数字经济发展,降低谈判成本与合规成本,获得更多全球竞争优势。对于中国而言,在与东盟跨境数据流动治理机制合作对接方面具备可能性和可行性,需要充分认识和评估东盟机制对世界数字治理的影响、对地区数字经济秩序的挑战,推动中国与东盟之间的数字经济合作。     

The killer outfit and timing: Impact of the fabric and time in body fluid identification and DNA profiling

The present work aimed to study the detection, through lateral flow immunochromatographic (LFI) tests, of saliva samples over time in three different types of fabrics, as well as, the possibility of DNA isolation and characterization from the sample tubes and the cassettes. Fifty microliters of saliva (three samples/time) were deposited in denim, cotton, and polyester. Saliva was identified by SERATEC® Amylase Test and the Crime Scene version SALIVA CS, being able to detect it up to six months of deposition, although with different band intensities. Polyester showed stronger bands than cotton, probably due to its synthetic nature, and denim, as an inked fabric, showed less band intensities. Statistical analyses confirmed significant differences among fabrics, but not over time in the same type of fabric. Total DNA from the sample tubes was successfully recovered, in contrast, from the cassettes, only polyester retrieved amplifiable DNA. These findings indicated that it is possible to recover and identify saliva up to six months after deposition, also obtaining DNA. Future research will be able to expand these results, analyzing the stability of other body fluids, and the sensitivity of lateral flow immunochromatographic tests to detect them.     

Forensic Analysis of MTBE Contamination Using Basic Hydrogeologic Concepts

Contamination of groundwater with petroleum hydrocarbons and additives, such as methyl tert‐butyl ether (MTBE), is often linked to the leaking product distribution system of gas stations. In very few cases is it know if and when a leak occurred and how much product was released to the environment. In the absence of direct evidence, a careful analysis of the available data, such as contaminant breakthrough at receptor wells or discrepancies in the product inventory data, may provide evidence about the nature of the release, its timing and magnitude. Using a MTBE contamination site in the formerly glaciated New England region as an example, two possible release scenarios (slow, long‐term release vs. spill) were examined. Of the two scenarios, the slow release could be ruled out as the sole source even though there was no direct evidence for a spill. The analysis of hydraulic test results together with chemical data further permitted to estimate when such an undocumented spill might have occurred. Analyses of the data also allowed these results to be compared to that of a prior transport and fate modeling study. Good agreement and consistency for contaminant travel times was confirmed. This forensic analysis demonstrates that applying basic hydrogeologic principles can aide in the reconstruction of contamination events while providing more readily understandable and defendable evidence relative to complex models. Conceptually, the approach described herein is transferable to other sites with similar hydrogeologies.     

缺血性中风气虚血瘀证动物模型的初步研究

选用中老龄大鼠，采用饥饿、劳累、高脂饮食、结扎左侧侧颈总动脉的方法，制作缺血性中风气虚血瘀证模型。通过对一般体征、局部脑组织血流量、脑水肿、血液流变学、血浆血栓素Ａ２（ＴＸＡ２０、前列环素（ＰＧＩ２）的观察。结果表明：模型鼠出现偏瘫症状；脑组织含 显著增高，脑水肿明显；ｒＣＢＦ显著增高，ＰＧＩ２显著降低，血液呈高度浓、粘、凝、聚状态。符合缺血性中风气虚血瘀证的临床特点。     

关于促进欠发达地区人才流动的思考

人才是一个国家经济和社会发展的重要战略资源,更是经济欠发达地区摆脱贫困、实现跨越式发展的关键。人才流动无论是在国家之间还是地区之间,都是加快人力资本积累进而促进经济社会发展的一个重要机制,是市场经济发展客观规律的必然要求。本文分析了欠发达地区人才流动的重要性及影响人才流动的不利因素,提出了促进欠发达地区人才流动的建议,即以经济结构转变吸引人才,以高质量社会服务水平支持人才流动,以政府政策导向引导人才流动。