A survey of bacteria associated with various life stages of primary colonizers: Lucilia sericata and Phormia regina

Blow flies are common primary colonizers of carrion, play an important role in the transfer of microbes between environments, and serve as a vector for many human pathogens. While some investigation has begun regarding the bacteria associated with different life stages of blow flies, a well replicated study is currently not available for the majority of blow flies. This study investigated bacteria associated with successive life stages of blow fly species Lucilia sericata and Phormia regina. A total of 38 samples were collected from four true replicates of L. sericata and P. regina. Variable region four (V4) of 16S ribosomal DNA (16S rDNA) was amplified and sequenced on MiSeq FGx sequencing platform using universal 16S rDNA primers and dual-index sequencing strategy. Bacterial communities associated with different life stages of L. sericata and P. regina didn't differ significantly from each other. In both blow fly species, Bacilli (e.g., Lactococcus) and Gammaproteobacteria (e.g., Providencia) constituted >95% of all bacterial classes across all life stages. At the genus level, Vagococcus and Leuconostoc were present at relatively high abundances in L. sericata whereas Yersinia and Proteus were present at comparatively high abundances in P. regina. Overall, information on bacterial structures associated with various life stages of blow flies can help scientists in better understanding or management of vector-borne pathogen dispersal and in increasing the accuracy of microbial evidence based postmortem interval (PMI) prediction models.     

Classical Marxism and Maoism: A comparative study

The issue of the Marxist character of “Mao Zedong Thought” has never really been resolved. The present work is a comparative analysis of the classical Marxism of Karl Marx and Friedrich Engels and the ideology of Mao Zedong. The argument is made that whatever Marxism there was in Maoism was the “creatively developed Marxism” of V. I. Lenin—which allowed for socialist revolution in retrograde economic circumstances—something that had been specifically rejected by Marx and Engels. That led to the theoretical idiosyncrasies that characterized Maoism throughout its history, and ultimately resulted in the form rejected by Deng Xiaoping and post-Maoist China.     

Market Demand for Civic Affairs News

This paper uses time series methods to test the relationship between civic affairs content and audience share for the ABC, CBS, and NBC evening news in the mid-1990s. Because these programs competed in the same market, but varied the amount of their civic affairs coverage, they present an excellent opportunity to analyze this relationship. The study uses hand-coded news abstracts and O.J. Simpson murder trial coverage as separate measures of news content. The study finds that lower levels of civic affairs content and higher levels of O.J. news are associated with greater audience share.     

利用16 S rDNA序列对两种芽孢杆菌的鉴定

根据不同种属细菌的16 S rDNA序列两端的保守性区域设计通用引物,提取菌株的基因组DNA,对菌株的16 S rDNA进行了PCR扩增,对扩增到的目标片段进行了测序,将测序结果与NCBI上已知菌种的16 S rDNA序列进行了BLAST对比,并构建了系统进化树.结合传统的形态观察及生理生化特性鉴定,16 S rDNA序列分析结果证实芽孢杆菌Pab02为枯草芽孢杆菌,PAS38为蜡样芽孢杆菌.     

Rechtsbezeugende Beurkundung eines mündlich abgeschlossenen (Bestand-)Vertrages in einem späteren Schriftstück

Ein zuvor mündlich abgeschlossener Bestandvertrag kann durch narrative Erw?hnung in der Pr?ambel eines schriftlichen Anbots (zur Vertrags?nderung) rechtsbezeugend beurkundet werden; Gebührenpflicht hiefür tritt ein, wenn das schriftliche Anbot dem Anbotempf?nger ausgeh?ndigt wird.     

Analoge Anwendung des Befristungsabschlags auf alle Hauptmietzinsvereinbarungen nach § 16 Abs 1 MRG

§ 9 Abs 4 RBG 1987 l?sst (nur) die Vereinbarung eines angemessenen Hauptmietzinses, definiert durch dieselben Merkmale wie in § 16 Abs 1 MRG, zu. Die Mietzinsbildungsvorschrift des § 9 Abs 4 RBG ist trotz der nicht deutlichen Formulierung auch eine Mietzinsbeschr?nkungsvorschrift. Die erforderliche Gleichbehandlung aller ausdrücklich oder inhaltlich dem § 16 Abs 1 MRG zu unterstellenden Hauptmietzinsvereinbarungen gebietet, die in § 16 Abs 7 MRG normierte Reduktion des angemessenen Hauptmietzinses auch im Fall einer Vereinbarung nach § 9 Abs 4 RBG 1987 vorzunehmen.     

荧光标记3个miniSTR基因座复合扩增系统的建立

目的建立扩增片段<135bp,包括D5S818,D8S1179,D16S539 3个miniSTR基因座复合扩增系统。方法采用不同荧光染料标记引物,通过PCR扩增,利用ABI 3100遗传分析仪进行片段长度分析,对100份无关个体血样,10个家系样本以及30份高度降解检材进行检测。结果本系统DNA分型结果与AmpFLSTR Identifiler试剂盒完全一致,且灵敏度高于AmpFLSTR Identifiler试剂盒。结论本系统可以应用于个人识别和亲权鉴定,为降解DNA样本分型提供了新的方法。     

贯彻十六大，全面建小康，公安怎么办？

在全面建设小康社会的伟大实践中如何发挥更大的作用,是新世纪新阶段对公安机关的严峻考验。本文从理论和实践的结合上进行了深刻思考,提出研究公安怎么办,必须以十六大精神为指导,紧紧围绕全面建设小康社会的宏伟目标做文章;必须着眼于当今世界形势的新变化,从忠诚履行公安机关三大历史使命上寻突破;必须适应新形势新要求,解放思想,开拓创新,在推进公安改革上下功夫。     

十六大精神与青年的历史责任

十六大是我们党在新世纪召开的第一次全国代表大会,也是党开始实施第三步战略部署、全面建设小康社会的一次重要代表大会。这次大会是一次高举邓小平理论伟大旗帜、全面贯彻“三个代表”重要思想的大会,具有深远的历史意义和重大的现实意义。当代青年要认真学习十六大文件.全面理解十六大精神,勇敢地肩负起历史赋予的使命,增强6个观念,为实现中华民族的伟大复兴建功立业。