Analysis of Δ-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography–electrospray ionization mass spectrometry

An analytical method using solid-phase extraction (SPE) and high-performance liquid chromatography–mass spectrometry (LC–MS) has been developed and validated for the confirmation of Δ⁹-tetrahydrocannabinol (THC) in oral fluid samples. Oral fluid was extracted using Bond Elut LRC-Certify solid-phase extraction columns (10 cm³, 300 mg) and elution performed with n-hexane/ethyl acetate. Quantitation made use of the selected ion-recording mode (SIR) using the most abundant characteristic ion [THC + H⁺], m/z 315.31 and the fragment ion, m/z 193.13 for confirmation, and m/z 318.00 for the protonated internal standard, [d₃-THC + H⁺]. The method proved to be precise for THC, in terms of both intra-day and inter-day analyses, with coefficients of variation less than 10%, and the calculated extraction efficiencies for THC ranged from 76 to 83%. Calibration standards spiked with THC between 2 and 100 ng/mL showed a linear relationship (r² = 0.999). The method presented was applied to the oral fluid samples taken from the volunteers during the largest music event in Portugal, named Rock in Rio-Lisboa. Oral fluid was collected from 40 persons by expectoration and with Salivette^®. In 55% of the samples obtained by expectorating, THC was detected with concentration ranges from 1033 to 6552 ng/mL and in 45% of cases THC was detected at concentrations between 51 and 937 ng/mL. However, using Salivette^® collection, 26 of the 40 cases had an undetectable THC.     

血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析

目的　建立血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析方法。方法　用 1%三氯乙酸 乙腈萃取血液样品 ,采用电喷雾离子源 ,正离子MRM扫描。结果　本方法线性相关系数r≥ 0 992 2。最低检测浓度(LOQ ,S/N =5 )分别为乌头碱 2 0ng/ml ,次乌头碱 0 5ng/ml ,新乌头碱 0 5ng/ml。空白血添加回收率 91 2 5 %～10 3 1%,变异系数 (CV ,n =6) <10 93 %。结论　LC/MS/MS法灵敏可靠 ,样品处理快速简便 ,适用于血液中乌头碱、次乌头碱、新乌头碱的检测。     

血中氯胺酮的液液提取及GC／ECD检测

通过采用液液提取技术以安定为内标对血中氯胺酮进行检验研究,同时考查了各种因素对提取率的影响,表明血中氯胺酮的最佳提取条件是:在pH10.8的碱性条件下,用乙醚提取,回收率为85.5%,最小检出限可达35ng/mL,并通过中毒实验,对中毒兔的血检材进行了分析,证明该方法准确、灵敏、可用于氯胺酮吸毒的认定。     

中毒死者体内毒鼠强的GC/MS-SIM分析

一 1 9岁男子赌气吞服一白色粉末 ,1 0min后口鼻流血、抽搐 ,迅速送医院 ,经催吐、透析等方法治疗 5天后死亡。根据中毒症状和经验怀疑为毒鼠强中毒。用GC/NPD检验肝中毒鼠强有干扰。改用GC/MS -SIM法分析 ,心血、肝和胃组织中全部检出毒鼠强 ,浓度分别为 0 5 1 μg .mL- 1、0 4 5 μg .g- 1和 0 33μg .g- 1,和文献结果在同一水平。GC/MS -SIM对复杂检材中低含量毒鼠强测定具有更高的选择性 ,也具有较高的灵敏度 ,同时进一步证实毒鼠强排泄较慢 ,经过较长时间后在体内分布比较均匀     

对故意传播艾滋病的刑事立法刍议

保障艾滋病病毒感染者和艾滋病病人的合法权利,使其得到应有的治疗,并规定他们应承担的避免将艾滋病毒传染给他人,包括亲人的义务。政府必须加快制定HIV感染者病人的行政和刑事管理法规,对“传播艾滋病罪”作出规定。     

信用证欺诈例外原则的问题分析

信用证是国际贸易中重要的信用结算工具,由于信用证独立抽象的原则,银行只审单而不管实际的货物,导致国际贸易中利用信用证进行欺诈的案件屡屡发生。因此,各国纷纷采取信用证欺诈例外原则来进行补救,但信用证欺诈例外原则的滥用则会损害合法受益人及善意第三方在信用证项下的利益,因此,对欺诈原则应适当地适用,以发挥其最佳效用。     

The Impact of Language Status as an Acculturative Stressor on Internalizing and Externalizing Behaviors among Latino/a Children: A Longitudinal Analysis from School Entry through Third Grade

This study examined the relationship between limited English proficiency status, and internalizing and externalizing behaviors among a sample of Latino/a children (N = 2,840) from the US Department of Education’s Early Childhood Longitudinal Study, Kindergarten (ECLS-K) data set. Results of cross sectional regression and hierarchical linear modeling (HLM) analyses suggest that there is a positive relationship between limited English proficiency and externalizing symptoms, particularly by third grade. Additionally, sex and place of birth also helped to explain externalizing behaviors at various time points in the models. Place of birth and family poverty were significantly associated with internalizing symptoms. Implications for future research and interventions related to internalizing and externalizing behavior among the Latino/a school aged population are discussed.

Comparing Putin’s and Brezhnev’s Policies toward the Middle East

This article compares Moscow’s and Washington’s foreign policies toward the Middle East in 1982 and 2008. In 1982, Moscow and Washington each had a distinct set of friends and foes. In 2008, Washington still has a distinct set of friends and foes, but Moscow has relatively good relations with all governments and most major opposition movements in the region—the only exceptions being Al Qaeda and its affiliates. It is argued that Putin’s policy toward the Middle East is not really aimed at displacing the U.S. in the region, but protecting Russia and Russian interests from Al Qaeda and its allies. Indeed, a continued American presence in the region serves to protect Russian interests in the region.

社区工作者与传统居委会干部之专业比较

社区建设的发展和深入 ,关键在于社会工作者的自身建设。本文从六个方面论述了社区工作者与传统居委会干部的不同 ,从而确立了社区工作者的专业角色特征