Optimization of the collection and analysis of touch DNA traces

The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR® NGMSElect™ kit and the Precision ID mtDNA Whole Genome Panel.     

Time and Punishment: Delayed Consequences and Criminal Behavior

This article develops two distinct explanations for the failure of potential consequences to influence behavior. Discounting is the tendency to deliberatively devalue the future. In contrast, poor impulse control refers to the failure to consider the future. The implications of this distinction were investigated with data from the National Longitudinal Survey of Adolescent Health. The study produced several findings. First, both forms of present-orientation independently predicted a range of problem outcomes among respondents. Second, high discounting was a better predictor of deliberative or future-related problem outcomes, whereas poor impulse control was a better predictor of urge driven behaviors or conduct involving little forethought. Third, only poor impulse control but not high discounting predicted violent offending among respondents. While both forms of present-orientation were associated with property offending, high discounting was a stronger and more consistent predictor. These three findings were far more evident for males than they were for females.     

The Life and Death of New Political Parties in the Low Countries

Numerous new parties have emerged since voters became less loyal to established political parties. A number of these survived and have been analysed intensely, especially green and radical right parties; many other new parties disappeared and have been neglected by party research. This article analyses the fate of all 30 political parties that entered parliament in the Netherlands or Belgium between 1950 and 2003. Qualitative comparative analysis is used to identify characteristics of both surviving and disappeared new parties. Conditions related to party origin (roots in civil society, organisational newness, initial programmatic profile) are scrutinised, as are conditions pertaining to the party’s developmental process (party organisational strength and the occurrence of defections or party splits). Surviving parties are characterised by strong, rooted organisations that have not suffered defections. Most disappeared parties lacked a strong organisation and roots and have experienced shocks that they could not absorb. Organisational newness makes new parties vulnerable.     

Evaluation of direct PCR for routine DNA profiling of non-decomposed deceased individuals

Forensic DNA profiling is a standard method used in the attempt to identify deceased individuals. In routine investigations, and if available, the preferred sample type is usually blood. However, this requires the invasive re-opening of the body, days or weeks after the autopsy, which is undesirable in resource-constrained mortuary settings. Motivated by the ease of sampling as well as reduced health and safety risks, this study aimed to establish the success rate of generating a full DNA profile on first attempt from buccal swab lysates using a direct PCR approach. Buccal swab samples were collected from 100 unidentified deceased males, and were subjected to direct DNA profiling with use of the Promega PowerPlex® Y23 Kit. At the time of sample collection, these individuals had been stored for between 1 and 887 days. This study shows that full DNA profiles were initially obtained from 73% of samples, which constitutes the first empirical data pertaining to first time success rates of direct PCR from post-mortem buccal lysates. Further investigation of partial and failed DNA profiles using real-time PCR showed that samples did not contain PCR inhibitors, DNA was not degraded, but DNA concentration was particularly low. Repeating DNA profiling with increased lysate input and extra PCR cycles yielded an additional six full DNA profiles, resulting in an overall success rate of 79%. Overall, DNA profile success rate was not associated with the duration of storage (p = 0.387). Lastly, massively parallel sequencing with the ForenSeq™ Signature DNA Prep kit provided more informative profiles for three additional samples. These results indicate that blood should therefore remain the sample of choice in a post-mortem setting, yet buccal lysates hold potential to be optimised further, which may ease the human identification workflow.     

提升纪检监察干部“五种意识”之文化自觉

中国共产党自诞生以来就致力于做到执政文化自觉。反腐倡廉是一项关系我党生死存亡的极其重要而且长远的艰巨任务。增强反腐倡廉能力和提高反腐倡廉的科学性,是新形势下全党的重大政治责任和任务。作为我党反腐倡廉忠诚卫士的纪检监察干部需要有非常过硬的拒腐防变的本领。对纪检监察干部的严格要求,是我党反腐倡廉的一个重要组成部分,更是我党保持永久先进性和纯洁性的重要的法宝之一。     

浅议低收入人群的医疗保障

当前,低收入人群的基本医疗保障存在一系列问题,应从保费征缴办法、起付设置、住院费垫支办法等方面进行改革,保障低收入人群的基本权益。     

Housing needs and effective policies in high‐tech metropolitan economies

Abstract

What are the most effective ways to provide low‐income housing to those left behind in new economy housing markets? Do winners and losers in high‐tech competition require federal housing strategies geared to metropolitan differences? This article examines 45 large metropolitan areas grouped along a high‐tech spectrum to see who is dis‐advantaged and to deduce effective local low‐income housing strategies from market characteristics.

Finding affordable housing was, on average, more difficult for low‐income renters and owners in high‐tech economies in the 1990s. Nonetheless, high‐tech metropolitan economies, like other local/regional markets, vary greatly. Sharp differences among and within metropolitan markets make it essential that federal strategies allow local policies to respond to local conditions. To most effectively provide low‐income housing to those left behind in all markets, federal policy should target sufficient resources to severe housing needs through many more vouchers and programs that permit and encourage effective local choices.     

Low self-control,gender, race,and offending in late life

Self-control theory has been one of the most scrutinized general frameworks of crime for over 20 years. A majority of evidence pertaining to the theory, however, is derived from samples of teenagers and young adults. Relatively little information exists regarding whether self-control explains offending among people in late adulthood. As such, the generality of the framework has yet to be fully examined. This study uses a representative sample of people aged 60 years and older from interviews conducted in Arizona and Florida. The current study tests two key propositions regarding the generality of the theory: (1) the extent to which self-control accounts for the relationship between demographic variables and criminal offending, and (2) the invariance thesis which stipulates that self-control will have a uniform effect on offending across social groups. The analyses reveal two findings regarding theoretical generality: (1) low self-control explains late-life criminal behavior but does not account for the relationship between offending and gender, and (2) low self-control has an invariant effect on offending across gender and race when measured behaviorally. Taken together, the analyses address important elements of the supposed generality of self-control theory and extend the framework's scope to the explanation of offending in late life.     

A cross-cultural validation of self-control theory

Several core propositions of self-control theory were tested utilizing self-reported data gathered from university students in Malta, Trinidad and Tobago, and the United States. The results provide some support for the claim that ineffective parenting decreases self-control and strong support for the proposition that self-control increases deviant behavior. Additionally, tests of slope differences for key theoretical variables across samples generated findings that are largely consistent with the theory's cultural invariance thesis. Implications of the findings from this multinational test are discussed.     

PCR扩增循环数与低拷贝模板DNA的STR分型

目的探讨PCR扩增循环数对低拷贝模板DNA的STR分型的影响。方法模板DNA(9947A)的不同扩增用量(含低拷贝模板量),采用ProfilerPlus试剂盒,扩增循环数分别为28、30、32、34、38次,3100型基因分析仪(ABI,美国)检测结果。结果循环数从28次增至38次,模板DNA量最低检出量可从0.25ng减少至0.0312ng;先循环28次后,每反应加0.3μlAmpliTaqGoldDNA聚合酶,再循环6次较1次34个循环的检测灵敏度高,相当于1次38个循环的效果。结论增加PCR扩增循环数,可能影响低拷贝模板DNA的STR分型。