Simi视频动作捕捉系统在步态识别中的应用

目的探讨人体行走运动主要步态特征参数新的提取识别方法和定量化分析技术。方法以步态分析技术与传统的足迹检验理论和方法为基础,运用先进的Simi Motion视频动作捕捉系统,对50名健康男性正常行走的步态特征参数进行识别和分析。结果提出了一种基于关节点的步态特征提取方法,得到了行走步态特征的基本参数,为步态特征定量化检验提供技术支持。结论研究表明,simi视频动作捕捉系统可以获得较精确的步态特征数据,能够满足人体步态分析的需求,进而达到人身个体识别的目的。     

This time it’s different: lithium extraction,cultural politics and development in Bolivia

As governments throughout Latin America have increased their dependence on resource extraction, the debate around extraction-based development has been reinvigorated. This article argues that, despite historical failures and recurrent conflicts associated with extraction-based development, the way in which development is experienced and conceptualised at the subnational level demonstrates why extraction continues to be perceived as a legitimate means for development. These findings show that, as resource extraction continues to play a critical role in the overall development transition of Latin America, the process must be understood and theorised in relation to the experiences and expectations of actors at multiple scales.     

Doctors Doing Gender at Eye Clinics—Gender Constructions in Relation to Waiting Times for Cataract Extractions in Sweden

Why do eye clinics differ in their waiting times for women’s and men’s access to cataract extraction (CE)/“grå starroperation”? Taking a doing-gender perspective as the starting point, this study explores how gender constructions embedded in Swedish eye clinics contribute to longer waiting times for women than for men. Focus group interviews were conducted with doctors at two Swedish eye clinics: one with a larger and another with a smaller than average gender difference in waiting times for CE. Several differences were found between the clinics regarding how gender was constructed: Women and men were constructed as different with respect to ascribed traits such as assertiveness and care-seeking behaviour. Their need for visual acuity in working life was perceived as different by the doctors, and the study indicates differences between the clinics regarding their interest and awareness concerning issues related to inequity, reflected in the dissimilar prevalence of jokes with racist and misogynist connotations at the two clinics.     

Α Simple Method for the Determination of Lacosamide in Blood by GC-MS

Lacosamide is a functionalized amino acid with antiepileptic function. Therapeutic drug monitoring (TDM) in patients for lacosamide is critical as it allows clinicians to control epileptic seizures. A single liquid–liquid extraction step was applied for the extraction of lacosamide from whole blood samples which were thereafter analyzed by GC-MS. Optimum extraction conditions were selected on the basis of experiments with various solvents at different pHs, indicating ethyl acetate at pH 12 as the most efficient parameters for the extraction of lacosamide. Method exhibited linearity from 2 to 100 μg/mL with R² = 0.998. Accuracy and precision were evaluated at three concentrations and found to be within acceptable limits. LOD and LOQ were determined at 0.1 and 0.5 μg/mL, respectively. Lacosamide was found to be stable at storage conditions. The developed method was applied successfully in clinical samples and postmortem blood sample from an overdose case.     

A Powder-free DNA Extraction Workflow for Skeletal Samples

The processing of skeletal material poses several challenges for forensic laboratories. Current methods can be laborious, time-consuming, require dedicated equipment, and are vulnerable to contamination. In this study, various sample mass (1 × 50 mg, 3 × 50 mg, and 1 × 150 mg chip(s)) and incubation times (2, 4, and 16 h) were tested using the PrepFiler^® BTA™ Forensic DNA Extraction Kit to digest whole bone chips in lieu of powdering. The most effective method was then applied to bones and tooth fragments collected from contemporary human cadavers exposed to various environmental conditions using an automated platform. Over a third of the samples tested generated full DNA profiles without having to powder the bone/tooth fragment or further alter the manufacturer's protocol. However, for most samples resulting in incomplete STR profiles due to low amounts of DNA, slightly better results were achieved with powdered tissue. Overall, this work demonstrates the potential use of a faster, nonpowdering DNA extraction method for processing skeletal samples as an effective first-pass screening tool.     

DPX-GC/MS法分析尿液中新精神活性物质二苯基乙基哌啶

目的 建立一种枪头式分散固相微萃取-气相色谱质谱法,分析尿液中的二苯基乙基哌啶.方法 对影响萃取效果的吸附树脂种类、淋洗液种类、洗脱液种类和洗脱液用量进行优化考察.结果 在最佳实验条件下,尿液中二苯基乙基哌啶的线性范围为0.02～1.0 mg/L,相关系数r2>0.99,检出限为0.005mg/L,回收率在76.7％～...     

从指甲中提取DNA进行PCR-STR初步研究

本文成功的提取了指甲中的细胞核DNA,并进行多个PCR-STR位点扩增,具有实际应用价值.     

鼠抗人转铁蛋白单链抗体基因序列测定及分析

从携带人转铁蛋白单链抗体基因载体转化的大肠杆菌菌株中提取质粒,经电泳分析及筛选、纯化出线状质粒DNA,定量后以载体上人转铁蛋白单链抗体基因插入子两边的已知DNA序列经合成后作为引物进行测序扩增凝胶电泳,将得到的人转铁蛋白单链杭体基因序列重轻链可变区部分同基因库中鼠抗体重轻链进行同源比对,证明人转铁蛋白单链抗体基因确由鼠抗体重轻链可变区基因构成,在此基础上,进一步对基因核苷酸序列的可变区结构特征作推断分析,并推导出其对应的氨基酸序列.     

圆珠笔油墨字迹书写时间鉴别的研究

圆珠笔油墨字迹书写时间的鉴别是摆在文件鉴定人员面前的棘手问题.本文作者借鉴国外的检验方法,经过近十年的探索,考查了各种因素的影响,研究出了适合我国国情的通过圆珠笔字迹油墨被溶解能力测试来确定其书写时间的鉴定技术,并且进一步发展了国外的检验方法,研制出了专用的检验器材,发明了模拟低温操作程序,使这一技术的适用范围更广.在有合适比对样本的情况下,可以准确鉴别落款时间在一年以上、怀疑书写时间在半年以内的可疑文件的真伪.     

鼠抗人转铁蛋白抗体轻链与重链可变区基因库的制备

以纯化的人转铁蛋白免疫小鼠,测其血清效价,合乎要求后杀鼠取脾脏并以异硫氰酸胍和酚的混合溶液将其匀浆,再以氯仿抽提、异丙醇沉淀和乙醇洗涤,所得总RNA以oligo(dT)纤维素离心柱分离纯化制备mRNA,使mRNA达到eDNA合成所需的浓度后,反转录制备出eDNA,在合成的eDNA中加入轻、重链引物,扩增制备出小鼠抗人转铁蛋白抗体的轻、重链可变区基因库。