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951.
目的研究色氨酸羟化酶(tryptophan hydroxylase,TPH)基因座-T457C位点在中国北方汉族群体中的遗传多态性及其法医学应用价值。方法应用PCR-RFLP、聚丙烯酰胺凝胶电泳及银染技术,检测180例北方汉族无关个体TPH基因座-T457C位点的遗传多态性。结果TPH基因座-T457C位点在中国北方汉族群体中,其个体识别能力(DP)为0.624,非父排除率(PE)为0.187,与法国人群基因频率比较差异显著(P<0.05)。结论TPH基因座-T457C位点在法医学中有重要应用价值,其遗传多态性可能存在民族或地域差异。 相似文献
952.
Masaru Asari Ph.D. Tomohiro Omura Ph.D. Chikatoshi Maseda Ph.D. Kazuo Matsubara Ph.D. Hiroshi Shiono M.D. Ph.D. Keiko Shimizu M.D. Ph.D. 《Journal of forensic sciences》2010,55(6):1576-1581
Abstract: We developed a new method for forensic ABO genotyping based on a universal reporter primer (URP) system. This allows for the simultaneous detection of six single nucleotide polymorphism (SNP) sites in the ABO gene (nucleotide positions 261, 297, 526, 703, 796, and 803). This URP system provides obvious peaks, ranging from 82 to 151 bp in length. ABO genotypes were classified and successfully genotyped by our method, including minor alleles that may cause a discrepancy between the genetic data and serological phenotypes. Full profiles were identified using as little as 0.1 ng (0.05 ng/reaction) of standard K562 and 9947A DNA. Moreover, the success rate of genotyping from a URP system was much higher than that from a conventional primer extension method in degraded DNA. This method enables simple and rapid detection of multiple SNP sites on human ABO genes and is highly specific and sensitive when using limited and degraded DNA. 相似文献
953.
954.
目的建立血浆中丁丙诺啡液相色谱/质谱(LC/MS)分析方法。方法在含有丁丙诺啡的血浆中,加入内标奋乃静,加pH10.8缓冲溶液,用401有机担体作吸附剂、三氯甲烷作洗脱剂固相萃取,N2挥干,用50μL流动相定容后进行LC/MS分析。色谱条件:Thermo Hypersil-HyPURITY C18(150×2.1mm,5μm),柱温:40℃,流动相:10mmol/LNH4AC(pH3.4)∶甲醇∶乙腈=36∶52∶12,流速:0.22mL/min。结果方法的线性范围为0.05~5.0μg/L(r=0.9998),定量限0.05μg/L,检出限0.01μg/L(S/N=3);3个浓度的质量控制样品(0.1μg/L,0.5μg/L,2.0μg/L)平均回收率分别为86.40%,92.72%,92.57%,RSD分别为4.51%,3.34%,2.09%。结论该方法操作简便、灵敏度高,可用于涉毒案件血浆中丁丙诺啡的分析。 相似文献
955.
Business Registers (BRs) are a very important information resource for investors, creditors, financial institutions and public authorities. The possibility to aggregate and interconnect these data at a European level could enhance the transparency of companies towards those actors and add a great deal of value to the raw Business Register data. The European BRITE project intended to provide adequate tools to meet these demands. BRITE will provide easier access and cross-border interoperability of Business Register data throughout Europe. On the other hand, the processing of BR data within the BRs and BRITE triggers several important European legislations such as the Data Protection Directive and the Directive on the re-use of public sector information. In this paper, the processing of BR data will be analysed from the perspective of both data protection and public sector information laws, analysing as well the relation between both regulations. Do these regulations strike an optimal balance between the interests of private data vendors to re-use BR data and enhance business transparency and the need to protect the personal data of natural persons? 相似文献
956.
Mobile customers are being tracked and profiled by behavioural advertisers to be able to send them personalized advertising. This process involves data mining consumer databases containing personally-identifying or anonymous data and it raises a host of important privacy concerns. This article, the first in a two part series on consumer information privacy issues on Profiling the Mobile Customer, addresses the questions: “What is profiling in the context of behavioural advertising?” and “How will consumer profiling impact the privacy of mobile customers?” The article examines the EU and U.S. regulatory frameworks for protecting privacy and personal data in regards to profiling by behavioural advertisers that targets mobile customers. It identifies potential harms to privacy and personal data related to profiling for behavioural advertising. It evaluates the extent to which the existing regulatory frameworks in the EU and the U.S. provide an adequate level of privacy protection and identifies key privacy gaps that the behavioural advertising industry and regulators will need to address to adequately protect mobile consumers from profiling by marketers. The upcoming second article in this series will discuss whether industry self-regulation or privacy-enhancing technologies will be adequate to address these privacy gaps and makes suggestions for principles to guide this process.1 相似文献
957.
中国互联网络信息中心(CNNIC)一直致力于互联网发展的调查和研究,每年两次的互联网统计报告全面、系统、客观、真实地展现了互联网的发展现状、网民的行为和习惯以及互联网发展的趋势,为我们开展互联网管理工作提供了有效的依据。因此,公安机关解读这个报告,能使我们对互联网有一个清晰的了解,寻找工作结合点和着力点,对作出准确的判断和科学的决策具有深刻意义。 相似文献
958.
汽车和挂车后下部防护装置安装使用现状分析 总被引:1,自引:0,他引:1
后下部防护装置是减少高速公路小车追尾货车事故的重要技术手段,但现阶段这一装置的应用状况普遍较差,导致社会危害和给社会造成的损失巨大。要从根本上改变目前这一状况,需要从多方面入手加以整治。 相似文献
959.
Anthony T. Quirk John M. Bellerby James F. Carter Fay A. Thomas Jenny C. Hill 《Science & justice》2009,49(2):87-93
A number of two-way radios, similar to those which have been employed to initiate Improvised Explosive Devices (IEDs), were acquired from a commercial supplier and grouped into four pairs. Samples of plastic material were collected from five distinct regions of each radio and analysed by Infrared and Raman spectroscopy to identify the nature of the material. One radio of each pair was then subjected to detonation with a commercially available plastic explosive. The combination of radio and explosive was considered to be representative of the components of an IED. Following detonation, fragments were recovered and, where possible, identified as specific sampling points of the radio.A combination of δ2H and δ13C stable isotopic analysis of material from each of the five sampling points was found to provide a pattern which was characteristic of a given radio and provided a means to associate pairs of radios. When few fragments were recovered, no positive association could be made between the fragments and the paired, undamaged radio. This was attributed, in part, to manufacturing variation in the radios. However, when three or more post-blast fragments were recovered it was possible to associate these with the paired, undamaged radio with a high degree of certainty. 相似文献
960.
Since the publication of analyses suggesting the significant impact on youth homicide of the Boston “pulling levers” intervention,
a series of studies of similar strategies have indicated promise in reducing homicide and gun assaults. One of these studies
was an assessment of a pulling levers strategy in Indianapolis, where trend analyses indicated a significant reduction in
homicide following the intervention, while six other similar Midwestern cities did not experience a significant decline in
homicide. We re-assess the results of the Indianapolis study by disaggregating the offenses into gang- and non-gang homicides.
Given that the pulling levers program focused on influencing gangs and networks of chronic offenders, the impact of the intervention
should be more apparent for gang homicides than for non-gang homicides. Alternatively, should the impact be similar for non-gang
homicides, then it is more likely that the downward trend would be caused by unmeasured external forces. Coefficient-difference
tests relying on estimates obtained from autoregressive integrated moving average (ARIMA) time–series models indicate that
gang homicides declined significantly more than did non-gang homicides following the Indianapolis intervention. These findings
suggest ‘something happened’ to gang homicides that did not happen to non-gang homicides, which adds further support that
the pulling levers initiative was the driving force behind the overall reduction in homicide in Indianapolis.
Nicholas Corsaro is an assistant professor in the Center for the Study of Crime, Delinquency, and Corrections at Southern Illinois University-Carbondale (SIUC). Prior to joining SIUC, he completed his Ph.D. at Michigan State University in 2007. Corsaro’s research interests include strategic approaches to reducing crime, ecological criminology, program evaluation, and quantitative statistical techniques. Recent articles have appeared in Victims and Offenders and Justice Quarterly. Edmund McGarrell is professor and director of the School of Criminal Justice at Michigan State University. McGarrell’s research interests are in communities and crime, with particular emphasis on the studying of problem solving responses to gun, gang, and drug market crime and violence. Recent articles have appeared in Justice Quarterly, Policing, Journal of Contemporary Criminal Justice, and the Journal of Criminal Justice. 相似文献
Nicholas CorsaroEmail: |
Nicholas Corsaro is an assistant professor in the Center for the Study of Crime, Delinquency, and Corrections at Southern Illinois University-Carbondale (SIUC). Prior to joining SIUC, he completed his Ph.D. at Michigan State University in 2007. Corsaro’s research interests include strategic approaches to reducing crime, ecological criminology, program evaluation, and quantitative statistical techniques. Recent articles have appeared in Victims and Offenders and Justice Quarterly. Edmund McGarrell is professor and director of the School of Criminal Justice at Michigan State University. McGarrell’s research interests are in communities and crime, with particular emphasis on the studying of problem solving responses to gun, gang, and drug market crime and violence. Recent articles have appeared in Justice Quarterly, Policing, Journal of Contemporary Criminal Justice, and the Journal of Criminal Justice. 相似文献