SYBR GreenI实时荧光PCR技术在人类DNA定量中的应用

目的建立SYBR Green I实时荧光定量PCR技术在法医物证检验中的应用。方法应用SYBR Green I实时荧光定量PCR技术对各种生物检材进行定量分析,并在此基础上进一步分析STR。结果得到了实验的各种生物检材的准确定量。结论SYBRSYBR Green I实时荧光定量PCR技术是一种高效且廉价的检测基因拷贝数的方法,具有法医学应用价值。     

新一代高通量测序技术及其法医学应用前景

随着大规模基因组学的兴起,多种高通量、低成本的新一代测序技术应运而生,并逐步被应用到生命科学研究的各个领域.本文对新一代测序技术进行综述介绍,并对其在法医学领域中的应用前景进行展望.     

GC/MS法测定人体血液和肾、肝组织中的丙泊酚

目的 采用气相色谱/质谱法检测人体血液和肾、肝组织中的丙泊酚.方法 心血和肾、肝组织中的丙泊酚经环己烷提取,采用气相色谱/质谱法,丙泊酚选择m/z 163、178,内标百里香酚选择m/z 150、135进行测定;并考察方法专属性、线性范围、定量线与检出限、回收率与精密度.结果 丙泊酚的保留时间为8.17min,与内标百里香酚分离良好;其在心血和肾、肝组织中的检测浓度线性关系良好,r值均大于0.992,最低检测质量浓度分别为0.05 μg/mL、0.10μg/g及0.05 μg/g,方法回收率90％ ～ 110％,日内、日间RSD均小于7％.结论 本文方法简便、灵敏、快速,其准确度、精密度和回收率均可满足生物检材中丙泊酚浓度的测定,可在相关研究及实践中选用.     

Accuracy and reliability of cone beam computed tomography of the jaws for comparative forensic identification: a preliminary study

Conventional computed tomography is an emerging modality in forensic identification but is not sufficiently accurate for use in dental identification primarily because of problems with metallic dental restoration-induced streak artifact. In this study, the accuracy and reliability of recording forensic information from cone beam computed tomography (CBCT) scans of the jaws when compared to conventional panoramic radiographs has been analyzed under experimental conditions. Information could be recorded with near-perfect repeatability and reproducibility. Information could also be recorded accurately, the sensitivity being 96.6% (95% CI, 95.1-98.1) and specificity being 98.4% (95% CI, 96.2-100). The metal dental restoration-induced streak artifact was at a level that permitted, in most cases, accurate observations. This is considered an important step in validating CBCT as a tool in comparative dental identification of bodies. It may have a role in mass fatalities and in chemical, biological, radiological, and nuclear incidents, but further studies are required to assess the feasibility of this.     

后Bilski时代电子商务商业方法专利之因应与挑战

美国法院早期以商业方法属于数学演绎方法为由,将商业方法排除在专利保护之外.受此影响,商业方法在美国一直被认为不应授予专利.后来法院提出如果抽象的商业方法与运用这种方法后发生了某种物理转换或者有形的装置相联系,则应承认其具有可专利性.1998年更是表明只要该项发明能导出“实用、具体、有形之结果”者,仍不失其可专利性,否认了商业方法在美国《专利法》中存在例外.虽然联邦巡回上诉法院在In re Bilski案中,提出美国《专利法》第101条规定的方法发明须符合“机器或转换测试法”,试图限缩商业方法专利的范围,但联邦最高法院在 Bilski v.Kappos案中否认其为审查商业方法专利唯一的判断标准.在迄今为止仍存较大争议的背景下,如果既无技术贡献,亦无技术特征,更未与其实现所必须的计算机设备或计算程序相结合,而仅系解决商业经营的程序、步骤或者流程者,商业方法即应为人类智力活动的规则或方法,属于思想的范畴,而不属于专利权的保护客体.     

Experimentalist governance in the European Union: A commentary

Sabel and Zeitlin's Experimentalist Governance offers an insight into European governance in those cases where the EU institutions do not have clear competence and where member states are not prepared to accept a unified policy on a problem at hand. Experimentalist Governance identifies four steps of action: agree on common goals, have lower levels propose ways to meet goals, then report on their meeting of goals, and, finally, periodically reevaluate the review procedures. By looking at the developments in EU policymaking through the lens of experimentalist governance (EG), one obtains an appreciation of how goals might be achieved that would otherwise not likely have been achieved through the community method. Sabel and Zeitlin highlight how EG can be effective in obtaining results, integrating peers, and incorporating deliberation, and offer a different way to deal with accountability and legitimacy. This article closes by taking the next step, namely, asking what challenges EG poses to democratic processes.     

茚三酮薰显法在人体接触细胞发现采集中的应用

目的 研究茚三酮薰显法在人体接触细胞发现采集中的应用价值.方法 对衣服、口罩、棍棒等可疑人体接触细胞检材258份,采用1％茚三酮溶液进行显色反应,然后用磁珠法提取DNA,并进行DNA定量和STR检测.结果 可疑人体接触细胞检材中有172份显色反应阳性,其中115份检出的DNA浓度在0.02ng/μL以上并检出6个以上STR基因座的基因型,检出率为66.86％;显色反应阴性的86份检材均未检出DNA浓度或成功进行STR基因型.结论 茚三酮薰显技术可用于指导案件人体接触细胞的发现采集.     

领导干部“德”的现状及其考核评价——以宝鸡市为例

领导干部的道德考核评价是中国共产党历代领导集体高度重视的重大课题。通过对宝鸡市领导干部"德"的问卷调查及数据分析发现,当前领导干部高度关注道德建设,对"德"的内涵认识明确;领导干部道德水平整体较高,认同道德考评体系和评价机制。但不容忽视的是仍存在道德价值观淡漠,理想信念、自我修养缺失,廉洁从政观念模糊等问题。为此,要以领导干部"德"考核评价中存在的问题为突破口,客观构建领导干部"德"的考核评价基本模式,夯实领导干部"德"考核评价的基础。     

非正规就业问题初探

在经济转轨过程中,我国非正规就业部门和非就业人员的数量增多.借鉴国际劳工组织的相关解释,对非正规就业概念进行界定,有利于针对目前我国非正规就业存在的问题,从统计方法上和政策层面上给出对策建议.     

试论提高工会维权能力的方式与机制

在加强党的执政能力建设中,认真研究和分析党的执政能力建设对工会工作提出的新任务、新要求,更好地发挥工会作为党联系群众的桥梁和纽带的重要作用,是当前工会工作中需重点研究和解决好的一个问题.要提高工会的维权能力,关键要做好两方面工作:一是转变维权方式,二是健全维权机制.