以BOT投资方式促进西部基础设施建设

作为一种特殊的直接投资方式 ,BOT对我国西部地区引进外资并加快当地的基础设施建设具有重要意义 ,为在西部地区充分利用BOT方式 ,有必要克服观念、体制及法律方面的不利因素。     

H5亚型禽流感病毒核蛋白基因重组质粒的构建及其在Hela细胞的瞬时表达

采用PCR技术从重组质粒pMel-NP扩增得到了禽流感病毒(AIV)A/Goose/Guang-dong/1/96(H5N1)株的核蛋白(NP)基因,将其亚克隆至真核表达载体pVAX1上。将提取的pVAX-NP阳性克隆转染Hela细胞,48 h后经间接免疫荧光试验和Western-blotting检测,NP基因在Hela细胞中已成功瞬时表达。表达产物的分子质量约为57 ku,它与H5N1亚型AIV多克隆抗血清有较好的免疫反应。     

加入WTO后中小企业核心竞争力的培育

加入WTO后，国外的商品可以长驱直入我国的消费市场，这使一些发展水平低的产业（尤其是中小企业）将受到较大的冲击。同时，国内市场的竞争将更加激烈。近几年来，我国的中小企业之所以能较快发展，很大程度还是得益于关税壁垒的调节和政府的宏观调控能力。而入世后，关税降低     

关于哈尔滨旅游产业发展的对策思考

哈尔滨市旅游产业的发展既有优势，又有劣势，存在难得的发展机遇，又面临严峻的挑战。总的来说，优势大于劣势，机会大于威胁，困难与希望同在。应扬长避短，把优势化为胜势，将哈尔滨建设成为旅游强市。     

A Monte Carlo simulation and sensitivity cost benefit analysis for use of nylon 4N6FLOQSwabs®

Forensic genetic laboratories are challenged with implementing innovation even if the benefits to operational performance are well demonstrated often because of internal budget constraints. A prospective cost–benefit analysis (CBA) could support justification for an increased budget by effectively demonstrating in a system-based approach the relatively small cost of increasing a laboratory budget can substantially reduce costs to society (both qualitatively and monetarily). A Monte Carlo simulation and sensitivity CBA was performed using a more expensive swab (i.e., nylon 4N6FLOQSwabs®) compared with a less costly cotton swab. Ranges of input values and tangible and intangible benefits were considered. The outcome is that the relatively small increased cost of using a nylon swab pales compared with the potential tangible and intangible benefits to the overall system. This approach provides a sounder basis for requesting additional funds to support implementation of technologies and better approximates realistic situations while accommodating uncertainty of input values.     

基层经侦工作机制“1+N”模式初探

基层经侦工作机制的有效延伸有助于提升经侦部门的整体作战效能,将会实现打击经济犯罪的法律效果与社会效果的统一,揭示经济犯罪的发案规律,从而为有效预防和精确打击经济犯罪奠定坚实的基础。针对目前基层经侦工作机制延伸中存在的经侦职能延伸不到位、经侦协作落实不到位、经侦基础工作不到位、经侦业务考核不到位、少数经侦民警综合素质不到位等问题,提出在基层经侦大队构建"1+N"模式,即"经侦大队+六室"延伸模式,强化基层经侦工作机制。在中心基层派出所、同级行政执法部门、大型商圈、大型企业、大队内部分别建立经侦工作室、协作室、咨询室、联络室、档案室、法制室等,实现经侦工作向基层、行政执法部门及商圈、企业等延伸。     

Determination of an effective housekeeping gene for the quantification of mRNA for forensic applications

The potential application of mRNA for the identification of biological fluids using molecular techniques has been a recent development in forensic serology. Constitutively expressed housekeeping genes can assess the amount of mRNA recovered from a sample, establish its suitability for downstream applications, and provide a reference point to corroborate the identity of the fluid. qPCR was utilized to compare the expression levels of housekeeping genes from forensic-like body fluid stains to establish the most appropriate assessment of human mRNA quantity prior to profiling. Although variability was observed between fluids and individuals, results indicated that beta-2 microglobulin exhibited the highest expression for all body fluids examined and across donors. A one-way analysis of variance was performed for housekeeping gene variability between donors (at the α, 0.05, significance level), and the results indicated significant differences for semen, vaginal secretions, and menstrual blood.     

2009 H1N1 Fatalities: The New Mexico Experience

Abstract: Histopathologic features of New Mexico 2009 H1N1 fatalities have not been representative of those reported nationwide. We retrospectively reviewed medical records of all New Mexico 2009 pandemic influenza A (pH1N1) fatalities (n = 50). In cases in which autopsy was performed (n = 12), histologic sections and culture results were examined. In contrast to previously published studies, the majority of our fatalities did not have diffuse alveolar damage (DAD) (2/12; 16.7%). Common findings included pulmonary interstitial inflammation and edema, tracheobronchitis, and pneumonia. Two cases had significant extra‐pulmonary manifestations: myocarditis and cerebral edema with herniation. The majority had a rapid disease course: range from 1 to 12 days (median, 2 days), and Native Americans were disproportionately represented among fatalities. These findings suggest that New Mexico H1N1 fatalities generally did not survive long enough to develop the classic picture of DAD. Pathologists should be aware that H1N1 may cause extra‐pulmonary pathology and perform postmortem cultures and histologic sampling accordingly.     

A U.N. Convention to Regulate PMSCs?

Abstract

In the last 20 years the ruthless competition for natural resources, political instability, armed conflicts, and the terrorist attacks of 9/11 have paved the way for private military and security companies (PMSCs) to operate in areas which were until recently the preserve of the state. PMSCs, less regulated than the toy industry, commit grave human rights violations with impunity. The United Nations has elaborated an international binding instrument to regulate their activities but the opposition of the U.S., U.K., and other Western governments—and from PMSCs, which prefer self-regulation—have prevented any advancement.