新生代农民工城市社区融入问题研究

社区建设应指向社区发展,而主体性的社区发展是"善治"和现代化程度的指标之一,同时直接影响到城市居民的生活水平和生活质量。城市治理的目标之一就是包容性。包容性城市要避免对某些弱势群体如农民、农民工的排斥。有效的社区融入,不仅体现了城市对新生代农民工的包容,而且能够有效促进城市发展,提升城市治理的内涵。社区通过均等化服务、利益关注和倾斜以及组织化参与等方式,推动新生代农民工融入城市社区。     

二代测序技术在Y染色体遗传标记分型中的法医学应用

法医遗传学领域常利用Y染色体的父系遗传特点,对非重组区遗传标记进行检测并用于亲缘关系鉴定、混合斑检测、家系排查以及种族推断等研究.目前毛细管电泳仍是应用最为广泛的检测技术,基于该技术的商业化检测试剂盒及数据分析处理系统十分成熟.随着生物信息量的增长,传统检测技术通量低的弊端逐渐显现,推动了法医DNA分型技术的革新.近年...     

Politicians and Civil Servants: Unfinished Business—The Next Steps Report,Fulton and the Future*

This article discusses the origins of the Efficiency Unit's ‘Next Steps’ Report, published 20 years ago which recommended the executive agency as an organisation for much of the British government. Within five years more than half the civil service had been transferred to work in agencies but the other major recommendation of the report – improving the management skills of the senior civil service received far less enthusiastic attention. From the Fulton Report, the Next Steps report to the Capability Reviews of current Whitehall the same problem is highlighted: there is still too little management competence within the public service despite the endorsement of the need for improvement by successive governments.     

The Roots of Stereotypes about Tourists

The unique characteristics of the first generation tourists and the nature of their interactions with the host community leads to the formation of enduring stereotypes. The first wave of mass tourists sees themselves as innovators, worldly, outward looking, risk takers who are different and somehow better than other members of society. Yet, in reality, they are relative latecomers to the world of international tourism, causing members of the receiving community to perceive them as laggards, inward looking individuals who are culturally and socially introverted, unworldly and resistant to change. The situation is exacerbated by package tour participation which is the typical way any new markets begins to travel. Unfortunately, packages produce a highly mediated experience between host and guest that intensify the sense of outsidedness felt by each group, which in turn create stereotypes.

第三代中央领导集体与理论武装

党的十三届四中全会 ,特别是党的十四大以来 ,以江泽民为核心的第三代中央领导集体提出并实施了用邓小平理论武装全党的战略任务。一是坚持和发展邓小平理论 ,为理论武装提供了强大的思想武器 ,具体体现在 :坚决维护和高举邓小平理论伟大旗帜不动摇 ;科学概括邓小平理论的科学体系 ;深刻阐述邓小平理论的历史地位和指导意义 ;运用邓小平理论研究新问题取得新成果。二是采取了一系列重大举措 ,把理论武装落到实处。不但有力地推动了建设有中国特色社会主义事业的顺利发展 ,而且为在实践中坚持、丰富和发展邓小平理论作出了创造性的贡献。     

1对同卵双生新生儿DNA甲基化谱差异分析

目的 探索1对同卵双生新生儿之间DNA甲基化谱的差异.方法 应用甲基化免疫共沉淀结合高通量测序法对1对同卵双生新生儿的DNA甲基化谱进行检测,分析基因组DNA甲基化特点及其之间的差异,筛选适用于法医学分析的甲基化位点.结果 两样本各获得7300万原始测序序列(raw reads)数据,与人类基因组参考序列比对,各得到4800万和5000万唯一比对reads,其中大部分分布在重复区域,且在Alu序列分布最为广泛.两样本DNA甲基化富集区域(peak)各检测到257 362条和197 272条,基因组覆盖率分别为6.53％和5.29％,分布在基因组不同区域,以中间内含子区含量最多.分析两样本甲基化差异区域得到2205条差异的甲基化序列,其中595条位于基因区域,1610条位于基因间区,从中筛选出113条序列,用于进一步深入研究其法医学应用价值.结论 本研究初步证实了DNA甲基化用于同卵双生子鉴定的可行性,为筛选同卵双生子DNA甲基化差异位点提供了基础数据.     

新生代农民工制度化政治参与问题分析

新生代农民工是我国公民政治参与的重要组成部分。但是,当前我国新生代农民工的政治参与面临着非制度化政治参与扩张、制度化政治参与弱化的困境。其主要表现为：一是新生代农民工的收入普遍偏低,制度化政治参与的物质基础不实;二是新生代农民工参与渠道受限,制度化政治参与的制度保障不力;三是新生代农民工政治文化相对落后,制度化政治参与的文化内化不深;四是城市居民对新生代农民工的排斥,制度化政治参与的社会认同感不强等。     

Sequence‐based Saudi population data for the SE33 locus

A set of 87 reference samples collected from the population of Saudi Arabia were sequenced using the ForenSeq™DNA Signature Prep Kit on a MiSeq FGx™. The FASTQ files contain the sequences of the SE33 STR, but are not reported by the ForenSeq™ Universal Analysis Software (UAS). The STRait Razor software was used to recover and to report SE33 sequence‐based data for the Saudi population. Ninety-six sequence-based alleles were recovered, most of which had previously reported motif patterns. Two unreported motif patterns found in three alleles and seven novel allele sequences were reported. We also reported a single discordance between the sequence-based data and the CE data that was due to the presence of a common TTTT deletion. SE33 had 130% more sequence-based alleles; the highest number of observed sequence variants were in alleles 27.2 and 30.2, which each had 7 sequence variants. The statistical parameters emphasize the usefulness of using the sequence-based data.     

DNA甲基化检测方法及其法医学应用研究进展

DNA甲基化是表观遗传标记的重要组成部分,参与基因表达的调控,在生物发育、衰老以及肿瘤学等领域受到广泛关注。由于具有相对稳定性、可遗传、含量丰富、随龄变化等特点,在法医学领域,DNA甲基化可以作为DNA序列相关经典遗传标记的有效补充,用于年龄推断、组织体液来源检测、同卵双生子的鉴定等。DNA甲基化的检测方法主要有基于甲基化敏感限制性内切酶、重亚硫酸盐转化以及甲基化CpG结合蛋白等原理而建立的一系列方法,近年研究表明,第三代测序技术也可用于DNA甲基化检测。本文就DNA甲基化检测方法及在法医学领域的应用研究进行回顾与综述,以期为法医学实践提供参考。     

番鸭细小病毒MDPV YZ株VP2和VP3蛋白基因的克隆和序列测定

根据国外已发表的番鸭细小病毒(MDPV)FM株基因组核苷酸序列设计了1对引物,应用PCR技术扩增了MDPV YZ株的VP2和VP3蛋白基因片段.将扩增后的VP2和VP3蛋白基因克隆到pCR 3.1 T载体上,MDPV YZ株基因大小为1764 bp,编码528个氨基酸,并对插入片段进行序列测定.测定结果表明,我国分离的MDPV YZ株蛋白基因序列与国外发表的序列的同源性为98.5%.