鸡肾型传染性支气管炎病毒的分离及其N基因片段的克隆和测序

通过形态学观察、动物回归试验、鸡胚接种试验、病毒干扰试验以及血凝试验分离鉴定了1株肾型鸡传染性支气管炎病毒(IBV)。在透射电子显微镜下,病毒粒子多呈球形,直径为80~120 nm,有囊膜,表面有冠状突起。鸡胚连续盲传至第 1~2 代,开始出现死亡或出现侏儒胚;分离株可显著干扰NDV在鸡胚中的增殖;病毒尿囊液无凝血活性,但经5 g/L胰蛋白酶处理后,能够凝集10 mL/L的鸡红细胞。利用RT PCR技术对分离株的N基因进行了扩增,经克隆、序列测定和分析比较,证实分离株为肾型IBV,命名为AH3 04株。     

鹅副黏病毒辽宁分离株HN基因的克隆与序列分析

采用RT-PCR技术对鹅副黏病毒辽宁分离株 DG-01 的 HN基因进行了扩增,获得了 1条1.8 kb的特异性条带。将此扩增产物克隆至pGEM-T载体,重组克隆质粒经鉴定后进行 DNA序列测定。测序结果表明,所克隆的基因片段长度为1 810 bp,含有1个1 716 bp的开放性阅读框架,编码571个氨基酸。核苷酸同源性分析表明:DG 01株与我国其他9株鹅副黏病毒的同源性为89.9%~95.1%;与国内外NDV HN基因的同源性为82.1%~95.2%,与国内标准强毒F48E9的同源性为84.7%,与Taiwan95和NL/96株的同源性分别为94.1%和95.2%。     

Malaysia: perception of contemporary China and its economic,political and societal determinants

This article looks at the Malaysian perception of the contemporary rise of China by focusing more on the country’s societal response rather than from a broad overall perspective of international strategic relations and diplomacy. The Malaysian society is seen as a complex multi-entity construct, constituted by often sharply differentiated fragments and sub-fragments which could exhibit vastly different responses to the implications of the rise of China. Within such a construct, perception of the rise of China and the appropriate Malaysian response are intricately entwined with domestic power politics, generational transition and governmental control over public discourse. Hence, the Malaysian perception of the contemporary rise of China is as complex as the Malaysian society itself, and what is revealed in official government policies and public discourses in the dominant mass media would fail to reflect the real depth of the issue if the intrinsic complexity of the Malaysian society is not taken into due consideration.     

伪狂犬病病毒gG-gD基因片段的扩增及克隆和序列测定

以伪狂犬病病毒(PRV)HB-9304株的基因组为模板,利用聚合酶链反应(PCR)对其gG-gD基因片段进行扩增,获得了预定大小的片段,将这一片段克隆到质粒载体pPK中.对重组质粒pPKGD进行限制性内切酶分析、PCR鉴定和克隆片段的序列测定,证实了克隆片段的可靠性.     

论非洲的集体人权观与第三代人权的确认

集体人权包括国内集体人权与国际集体人权两大类,它源于20世纪40-50年代开始的民族独立运动.对集体人权的确认,是全球性非殖民化运动的结果.非洲人民系统地提出了民族权的概念和内容,全面关注集体人权,尤其是对以发展权和自决权为核心的第三代人权的确认和发展作出了杰出贡献.其集体人权观主要体现在以<非洲人权和民族权宪章>为主体的非洲人权法中.     

Internet as a source of information for tourists The case of Croatia

This paper presents the results of empirical research conducted during the summer 2007 on the Dalmatian coast in Croatia about how much tourists used the Internet as a source of information. Precisely, because tourism is an important part of Croatian economy, it is in everyone＇s interest for information about Croatia as a tourist destination to be available to potential guests. A variety of media are used in achieving that aim--starting with conventional speech and conversation amongst friends, through radio, television and the press, and now the Internet. This paper has the aim of exploring the share of Internet usage as a source of information compared to other media and showing some characteristics of tourists who have used the Internet as the main source of information and spent their vacations in Croatia in July and August in 2007. The main hypothesis is the continuing problem of the generation and education gap and that the Internet does not jeopardize direct communication.     

中国新生代群体内潜在冲突的现状与对策探讨——以“80后”农民工和“80后”大学生为例

作为中国新生代群体的主体,“80后”农民工和“80后”大学生已步入历史舞台。客观地讲,由于先天教育不平等造成的差距进一步引起后天职业地位竞争的不平等,二者在获得较好职业地位的能力上是存在一定差距的。但对于权利意识和平等观念日益提高和增强的新生代农民工群体而言,他们显然不能接受这一现实。对新生代群体的形成、追求及其在未来的社会发展中可能面临的群体间的矛盾进行分析,可进一步探求有效解决这一潜在矛盾与冲突的对策和机制。     

网络犯罪的心理成因与心理预防

网络犯罪作为一种新型的犯罪,刑法学家、犯罪学家、社会学家、伦理学家等各科专家学者们都从自己的专业角度分析了网络犯罪的成因,提出了预防网络犯罪的手段。同样,网络犯罪也有其自身的心理原因,并可以通过健全心理、矫正心理,达到预防犯罪、减少犯罪的目的。     

鼠抗人转铁蛋白单链抗体基因序列测定及分析

从携带人转铁蛋白单链抗体基因载体转化的大肠杆菌菌株中提取质粒,经电泳分析及筛选、纯化出线状质粒DNA,定量后以载体上人转铁蛋白单链抗体基因插入子两边的已知DNA序列经合成后作为引物进行测序扩增凝胶电泳,将得到的人转铁蛋白单链杭体基因序列重轻链可变区部分同基因库中鼠抗体重轻链进行同源比对,证明人转铁蛋白单链抗体基因确由鼠抗体重轻链可变区基因构成,在此基础上,进一步对基因核苷酸序列的可变区结构特征作推断分析,并推导出其对应的氨基酸序列.     

粪便DNA提取及检验

目的　研究人类粪便DNA的提取和检验方法。方法　 8人份粪便样本 ,磁珠法提取DNA后 ,进行STR复合扩增和mtDNAHVI区测序分析。结果　用 2种方法提取的粪便DNA ,STR复合扩增检验均未获成功 ;方法1提取的粪便DNA有 6个样本、方法 2有 7个样本获得了清晰可读的mtDNAHVI区序列 ,并与唾液对照样本DNA的序列完全一致。结论　用本文建立的方法提取粪便DNA ,不适于STR分析 ,可通过mtDNA测序分析进行检验。