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11.
Evaluation of Skin Surface as an Alternative Source of Reference DNA Samples: A Pilot Study 下载免费PDF全文
Mohammed H. Albujja M.Sc. Abdul Aziz Bin Dukhyil Ph.D. Abdul Rauf Chaudhary M.Phil. Ahmed Ch. Kassab Ph.D. Ahmed M. Refaat Ph.D. Saranya Ramesh Babu M.Sc. Mohammad K. Okla Ph.D. Sachil Kumar Ph.D. 《Journal of forensic sciences》2018,63(1):227-233
An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis development. The aim of this study was to evaluate skin surface cells (SSC) as an alternate source of reference DNA sample. From each volunteer (n = 10), six samples from skin surface areas (forearm and fingertips) and two traditional samples (blood and buccal cells) were collected. Genomic DNA was extracted and quantified then genotyped using standard techniques. The highest DNA concentration of SSC samples was collected using the tape/forearm method of collection (2.1 ng/μL). Cotton swabs moistened with ethanol yielded higher quantities of DNA than swabs moistened with salicylic acid, and it gave the highest percentage of full STR profiles (97%). This study supports the use of SSC as a noninvasive sampling technique and as a extremely useful source of DNA reference samples among certain cultures where the use of buccal swabs can be considered socially unacceptable. 相似文献
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Bradley MJ Gauntt JM Mehltretter AH Lowe PC Wright DM 《Journal of forensic sciences》2011,56(3):606-611
Fracture matches are considered the strongest conclusion in the forensic examination of rigid materials, such as glass, metal, and paint. However, publications that support the fracture matching of polymeric films, such as tape backings, are limited. This study was designed to determine the validity and error rate associated with conducting end-match (fracture match) examinations on vinyl electrical tape. Test designs varied the source roll of tape, test preparer, or mode of separation from the roll. Results indicated that each affected the resulting severed tape ends. The analysts examining the end matches also had an effect on the results. Eight end matches in the study were not identified by the initial analysts and were considered inconclusive. One end match was misidentified, resulting in one false positive and an error rate of 0.049%. These results support a comprehensive physical and chemical tape comparison regardless of indications of an end match. 相似文献
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Adhesive tape is commonly used in crimes and is often the subject of forensic evaluation. DNA analysis of adhesive tape can provide DNA profiles of suspects. The object of this study was to evaluate the applicability of DNA analysis on adhesive tape samples in forensic casework. We retrospectively reviewed all cases involving adhesive tape or similar items received by our institute for DNA analysis during the past 11 years. From 100 forensic cases reviewed, 150 adhesive tape samples were examined. A total of 98 DNA profiles were obtained from these samples. Sixty-two of the profiles provided feasible case-relevant information. In conclusion, DNA profiling of adhesive tape samples can be useful in a variety of forensic cases. 相似文献
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双面胶带是我们生活中常用的一种胶带,在许多案件中经常出现。本文主要研究双面胶带转印在纸张等渗透性客体上的手印来间接地显现双面胶带上的指印,从而认定作案的犯罪嫌疑人。 相似文献
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灰尘手印提取一直是现场勘察中关注的焦点,为了探索方便、快捷、有效地灰尘手印的显现方法,用“502”熏显不同客体、不同遗留时间的灰尘减层手印和灰汗混合手印,加染后用透明胶带粘取,然后透光照相。结果表明,显出手印清晰、流畅,不破坏手印。 相似文献
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An Optimized DNA Analysis Workflow for the Sampling,Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints 下载免费PDF全文
April D. Solomon M.S. Madison E. Hytinen B.S. Aryn M. McClain M.S. Marilyn T. Miller Ed.D. Tracey Dawson Cruz Ph.D. 《Journal of forensic sciences》2018,63(1):47-57
DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep? columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases. 相似文献
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为了更好地解决粘贴在普通A4纸上的胶带剥离与胶粘面指纹同时显现问题,本文对不同剥离试剂的剥离效果、不同显现试剂的显现效果以及不同胶粘面汗潜指纹遗留时间的影响进行了比较。实验结果表明,丙酮与无水乙醇按照1:10混合而成的试剂剥离效果最好;聚乙二醇和上海牌214型碳素墨水按照1:15混合而成的试剂显现效果最好;遗留5-7天的胶粘面汗潜指纹经染色2次后显现效果最佳,10-20天的实验样本经染色3次后显现效果最佳。 相似文献