鸡血清异种凝集素的应用研究

通过实验研究，证明家养鸡血清小含有“抗Ａ”、“抗Ｂ”及“抗Ｈ”三种异种凝集素。鸡血清中“Ｈ”凝集素能应用于体液班（唾液斑、精燃）Ｈ物质的检测。我们已在教学及检案实践小使用，效果良好。     

单亲鉴定案例STR选择探讨和PI值计算方法评价

作者对STR位点的个体识别能力(DP)、杂合度(H)、非父排除率(PE)、多态信息含量(PIC)等进行统计学分析,探寻了适合中国人群单亲子鉴定、且有利于国内外DNA实验室数据交流的STR位点,并对现行的PI值计算方法进行了评价。     

论完善我国亲权鉴定制度

本文着重探讨我国目前亲权鉴定领域内存在立法缺位、技术标准不统一、鉴定结论审查不严以及由此引起的诸多现实问题。提出确立防止亲权鉴定滥用原则、建立亲权鉴定质量保证和质量控制体系以及制定对亲权鉴定结论的严格审查采信制度这三个方面进行制度完善。     

Introduction of a standardized "paternity index" for the statistical evaluation of blood group findings in paternity testing

The introduction of a standardized paternity index (PI) for the statistical evaluation of blood group findings in cases of disputed paternity is proposed and explained. By using the PI $\text{X}\text{Y}$ as parameter, it is not necessary to give the information of the probability of paternity in percent. The PI includes the full information of the blood group findings. In addition to that, by using the suggested standardization based on the probabilities of error according to Schulte Mönting and Walter the test volume is also taken into account. The interpretation of the mathematical result is given by verbal predicates, the limitations of which are orientated by the verbal predicates for the probabilities of error according to Schulte Mönting and Walter, published by us elsewhere. Besides the essential fact that the test volume is taken into account, the most important advantage of this procedure is that the mathematical result is involved in the court decision only by the PI (which is free of any valuation) and its verbal predicate and not by sometimes relatively high percentages, which may be misunderstood by laymen.     

用α-珠旦白-3’HVR探针研究DNA指纹

用α-珠蛋白-3’HVR探针,经Southern印迹法,对100名不相关个体及4个家系的32名相关个体的DNA指纹进行了检测,所产生的DNA图谱具有高度的个体特异性,在被测的所有个体中无一相同。经统计学计算表明,任意两个个体DNA指纹图重合率为10~(-11)。家系分析毒明,DNA片段严格按照孟德尔方式遗传。该探针的应用,将在法医学亲子鉴定和个人同一认定中发挥重要的作用。     

A high selective screening test for methamphetamine in human urine

A high selective screening test (Ad-Tip method) for methamphetamine in human urine has been devised. The method involves a brief extraction from a urine sample with an Ad-Tip (ODS-silica minicolumn), washing, eluting with modified Simon's reagent, and coloration with carbonate buffer. Detection limit of methamphetamine in urine is 1 microgram/ml and the test takes within 3 min/sample. The results of the Ad-Tip method were almost identical to those of laboratory analyses.     

番鸭呼肠孤病毒乳胶凝集试验检测方法的建立

建立了用纯化的抗番鸭呼肠孤病毒(DRV)抗体致敏乳胶检测DRV抗原的方法。通过试验,确定抗体致敏乳胶的最佳浓度为1∶10(IgG蛋白浓度为0.424 2 mg/mL),最佳致敏温度为37℃,最佳致敏时间为120 min。用所建立的方法对人工感染的1 日龄雏番鸭30 只进行粪便检测,结果表明,感染后第3 d粪便中即可检测到病毒,直至人工感染鸭全部死亡都可从粪便中检出病毒抗原;对同样人工感染的30只1日龄雏番鸭的心、肝、脾病料用所建立的方法检测,结果表明,感染后第4 d,2/3番鸭脾病料检出阳性;感染后第5 d,2 只死亡鸭中有1 只肝病料检出阳性,脾病料2只都呈阳性;此后的病死鸭脾和肝病料均为阳性,而心则未检出阳性。     

Alpha-amylase kinetic test in bodily single and mixed stains

Recently, in Italy, a murder and a putative sexual violence was accomplished on a child. A bodily fluids mixture on the child's underwear between the victim (female) and the suspect (male) was ascertained by short tandem repeat (STR) DNA typing and, due to the absence of seminal fluid, saliva from the suspect and urine from the child was hypothesized. In order to investigate the possibility of specifically and rapidly detecting saliva stains both alone and mixed with other bodily fluids, we used a quantitative spectrophotometric technique, named Amylase test, for the detection of alpha-amylases. We determined alpha-amylase activity and reaction kinetic curves in several samples collected from the child's underwear. In order to confirm our intuition, we first tested saliva, perspiration, and urine, singularly and in mixtures; second, several forensic stains including saliva, perspiration, urine stains, saliva/perspiration, and saliva/urine mixture stains were tested. Evaluating alpha-amylase activity values and time-course curves' behavior of alpha-amylase reactions we were able to recognize successfully, in all cases, the presence of saliva and to distinguish it specifically from other bodily fluids containing alpha-amylase. A further confirmation of our result was provided by STR DNA typing on several areas of the underwear: a clear correlation between alpha-amylases activity and male DNA was detected on all the samples evaluated.     

欧美并购控制法实体标准比较研究

实体标准是整个并购控制体系的核心 ,也是反垄断法的重要内容之一。欧盟最新并购控制立法确立的“严重妨碍有效竞争”实体标准与美国奉行的“实质性减少竞争”标准一样都反映着并购控制的本质。欧美采取大同小异的并购实体标准有利于推动国际跨国并购审查结果的一致性 ,促进并购活动的发展。我国应确立“严重妨害正当竞争”实体标准并配套颁布并购评估指南 ,注重并购审查的经济分析