大鼠闭合性脑损伤后MAP-2的时序性表达

目的探讨大鼠闭合性颅脑损伤后脑组织微管相关蛋门－2（MAP－2）表达变化规律。方法建立大鼠闭合性颅脯挫伤模型,64只Wistar大鼠随机分为损伤后0．5h、6h、12h、ld、3d、7d、14d7个损伤组及对照组,每组8只。应用免疫组化和蛋h印记Western blot法及图像分析技术,检测脑挫伤后各设定时间点挫伤脯组织中MAP－2的变化。结果免疫纽化染色显示：对照组大鼠脑组织中可见MAP－2阳性表达;实验组伤后0．5h,挫伤灶及周同MAP－2阳性表达下降,伤后6h阳性表达降至最低,伤后12h,阳性表达逐渐增多,伤后14d,仍保持较高水平;蛋白印记结果与免疫组化结果一致。统计学分析显爪：MAP－2的表达各损伤组与对照组比较,差异均有统计学意义（P〈0．05）,伤后12h、3d及14d与上…组比较,差异有统计学意义（P〈0．05）。结论大鼠脑挫伤后14d内,MAP－2存脑组织挫伤灶及其周同呈现先减少后逐渐增多的表达变化,可作为推断脑挫伤经过时间的参考指标之一。     

外伤性癫痫法医学评定100例分析

目的探讨颅脑外伤后癫痫的法医学评定要点,提出外伤性癫痫的法医学评定依据。方法对100例颅脑损伤后癫痫患者门诊及住院病历,结合临床脑电图（EEG）结果、CT片、MRI片等影像学资料,进行了回顾性分析。结果依据GBl8667-2002《道路交通事故受伤人员伤残评定》标准有关颅脑损伤后癫痫发作类型、发作频率的相关条款规定,Ⅲ级伤残8人,Ⅴ级伤残10人,Ⅶ级伤残22人,Ⅸ级伤残40人,Ⅹ级伤残20人。误工期限、营养期限和护理期限评定结论时间长短差异较大,具有个体化特征。结论外伤后癫痫常继发于严重的颅脑损伤,其发作特点决定于颅脑外伤的部位和程度,外伤性癫痫法医学鉴定必须以外伤史及既往史为基础,结合电生理及影像学检查,综合癫痫发作类型、频率及药物控制情况作出正确的伤残等级及误工期限、护理期限、营养期限的鉴定结论。     

How important are interview methods and questionnaire designs in research on self-reported juvenile delinquency? An experimental comparison of Internet vs paper-and-pencil questionnaires and different definitions of the reference period

There has been relatively little change over recent decades in the methods used in research on self-reported delinquency. Face-to-face interviews and self-administered interviews in the classroom are still the predominant alternatives envisaged. New methods have been brought into the picture by recent computer technology, the Internet, and an increasing availability of computer equipment and Internet access in schools. In the autumn of 2004, a controlled experiment was conducted with 1,203 students in Lausanne (Switzerland), where “paper-and-pencil” questionnaires were compared with computer-assisted interviews through the Internet. The experiment included a test of two different definitions of the (same) reference period. After the introductory question (“Did you ever...”), students were asked how many times they had done it (or experienced it), if ever, “over the last 12 months” or “since the October 2003 vacation”. Few significant differences were found between the results obtained by the two methods and for the two definitions of the reference period, in the answers concerning victimisation, self-reported delinquency, drug use, failure to respond (missing data). Students were found to be more motivated to respond through the Internet, take less time for filling out the questionnaire, and were apparently more confident of privacy, while the school principals were less reluctant to allow classes to be interviewed through the Internet. The Internet method also involves considerable cost reductions, which is a critical advantage if self-reported delinquency surveys are to become a routinely applied method of evaluation, particularly so in countries with limited resources. On balance, the Internet may be instrumental in making research on self-reported delinquency far more feasible in situations where limited resources so far have prevented its implementation.

Real-time polymerase chain reaction quantification of canine DNA

The accurate quantification of target DNA is an important step in the short tandem repeat analysis of forensic biological samples. By utilizing quantification data to control the amount of template DNA in the polymerase chain reaction (PCR), forensic scientists can optimize testing and minimize the consumption of limited samples. The ability to identify and quantify target DNA in mixed-species samples is crucial when it may be overwhelmed by nontarget DNA, as in cases of dog attack. We evaluated two quantitative real-time PCR assays for dynamic range, species specificity, and inhibition by humic acid. While both assays proved to be highly sensitive and discriminating, the Melanocortin-1 Receptor (MC1R) gene Taqman assay had the advantages of a shorter run time, greater efficiency, and safer reagents. In its application to forensic casework, the MC1R assay has been advantageous for quantifying dog DNA in a variety of mixed-species samples and facilitating the successful profiling of individual dogs.     

计算被征收不动产价值的时间标准——美国法上的情形及其对解决“潘蓉案”的启示

被征收不动产的价值是变动的,征收补偿时,必须确定计算被征收不动产价值的时间标准。上海"潘蓉案"的重要争点之一就是应以潘蓉房产在何时的价值为补偿标准。美国法典没有对该问题的规定,部分州立法规定了计算被征收不动产价值的时间标准。美国法院一般尊重这些成文法,但如果在个案中适用成文法导致明显不公正,会援引《美国宪法第五修正案》中的公平补偿条款对时间标准做出微调。美国法的做法对我们解决"潘蓉案"有较大的启示意义。     

超期羁押问题的思考及对策

超期羁押是当前困扰我国司法实践的一大问题,司法制度的不完善是造成超期羁押的主要原因。文章从司法体制和实践方面对超期羁押问题提出了解决对策。     

Visualization of Aged Fingerprints with an Ultraviolet Laser

Detection of aged fingerprints is difficult because they can degrade over time with exposure to light, moisture, and temperature. In this study, aging fingerprints were visualized by time‐resolved spectroscopy with an ultraviolet‐pulsed laser. Fingerprints were prepared on glass slides and paper and then stored under three lighting conditions and two humidity conditions for up to a year. The fluorescence intensities of the fingerprints decreased with time. Samples were stored in the dark degraded less than in sunlight or under a fluorescent lamp. Samples were stored under low humidity degraded less than under moderate humidity. As the storage period increased, a fluorescence emission peak appeared that was at a longer wavelength than the peak visible in earlier spectra. This peak was used for visualization of an aged fingerprint over time. An image of the fingerprint was not initially visible, but an image appeared as the time since deposition of the fingerprint increased.     

死亡人体胸肌组织中rRNA亚基的稳定性评价

目的观察尸体胸大肌组织中rRNA各亚基表达水平的死后稳定性,探讨其在死亡时间推断中应用的价值。方法选取6例（3例成人、3例婴幼儿）外界温、湿度环境基本一致、死亡时间基本一致（24h以内）的个体,提取左侧胸大肌组织,在PBS缓冲液中低温冻存,分别在尸检即刻、2、3、4、5、6、7、8、9、10d提取微量组织保存于RNA固定液中,通过实时荧光定量RT-PCR技术检测rRNA 5srRNA、5.8srRNA、18srRNA、28srRNA亚基的表达水平。并分析年龄、死因对rRNA亚基表达水平的影响。结果死后各时间点rRNA各亚基的表达随死后经过时间延长无显著性降解,Ct值变化与死后经过时间无相关性（P值均大于0.05）,其中5s rRNA在18.503±2.655~20.937±2.340之间,5.8s rRNA在17.687±3.011~20.617±2.204之间,18s rRNA在16.457±3.920~22.330±2.571之间,28s rRNA在15.077±6.051~22.207±2.685之间。结论人体胸肌组织中rRNA各亚基稳定性好,其各亚基的表达量与死因、年龄无关,适于作为晚期死亡的推断的内参基因。     

How reliable are eyewitness memories? Effects of retention interval,violence of act,and gender stereotypes on observers' judgments of their own memory regarding witnessed act and perpetrator

An experiment was conducted to evaluate the effects of (i) stimulus person's gender, (ii) type of act (neutral or violent), and (iii) retention interval (short or long) on observers' memory of a stimulus person. Participants were presented with one of two acts: neutral (walking around in a store) or violent (robbing a store). The retention interval was 10 minutes or one–three weeks. The dependent variables were questionnaire items concerning the participants' memory of (1) the stimulus person's appearance and (2) the event, and (3) rating scales where the participants were asked to evaluate the stimulus person's aggressiveness, insensitivity, and other personality traits as well as characteristics of the act. Results showed that when the act was violent, and a long retention interval was used, a female, but not a male, stimulus person was evaluated less harshly than with a short retention interval (enhancement of gender stereotype); a stimulus person was seen as behaving in a more masculine way when performing a violent rather than a neutral act; witnessing the violent act resulted in better self-rated memory of the stimulus person; and with increasing retention interval, the violent act was seen as less negative and the neutral act as more negative (regression toward the mean).     

The Time Path of Legislative Party Switching and the Dynamics of Political Competition: The Italian Case (1996–2011)

Political competition is more realistically described as a dynamic process rather than as a series of static stages in which parties compete over policy and government formation. This paper focuses on legislative party switching as the main manifestation of this endogenously evolving process, linking individual switching behaviour to policy and office incentives that are assumed to evolve throughout the life of the entire legislature. Using a new data set tracking the timing of MPs’ changes in party affiliations between 1996 and 2011 in Italy, it is found that switching is mainly motivated by policy reasons and that it is more likely during government formation periods and budget negotiations. These results are a consequence of the interplay between MPs’ ambition and the alternation of key phases in the legislative cycle.