小鼠骨骼肌细胞核DNA降解与死亡时间的关系

目的　监测小鼠死后骨骼肌核内DNA降解的情况 ,探讨死后细胞核DNA降解的一般规律。方法　建立小鼠死亡模型 ,在死后 72h内 ,以 12h的间隔取骨骼肌样本进行单细胞凝胶电泳 ,在荧光显微镜下测量彗星图像 ,并作统计学分析。结果　机体死后 ,骨骼肌细胞在电泳图像上出现了明显的彗星形拖尾 ,L/W比值随死亡时间而逐渐增大 ,二者呈一定的线性关系。结论　单细胞凝胶电泳技术可以应用于早期死亡时间推断。     

Rapid postmortem changes of rat striatum dopamine, serotonin, and their metabolites as monitored by brain microdialysis

Brain microdialysis was used to monitor changes in extracelluar dopamine (DA), serotonin (5-HT), and their metabolite levels in the rat striatum at death by cervical dislocation. Maximum respective 450-fold and 150-fold increases in the extracelluar output of DA and 5-HT were observed within the first 30 min of death. DA and 5-HT outputs remained elevated over the following 2 h at levels about 100-fold and 50-fold above pre-death values, respectively. In contrast with monoamine outputs, the outputs of the DA metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), and the 5-HT metabolite, 5-hydroxyindoleacetic acid (5-HIAA), rapidly decreased by 10% and 20%, respectively 1 h after death. 5-Hydroxytryptophol (5-HTOL) gradually decreased after death. Before death both the extracellular DOPAC/DA and 5-HIAA/5-HT ratios were about 400; after death these ratios dropped to 0.56 and 4.0, respectively at 30 min. These observations suggested that regulation of neurotransmitter releases through the neuronal membrane and metabolisms in the rat striatum were seriously disrupted at death. This finding may be helpful in the determination of death in the field of forensic medicine.     

人心肌梗死心肌肌钙蛋白T脱失的免疫组化观察

目的 观察人梗死心肌中心肌肌钙蛋白T的脱失情况，探讨其在人心肌梗死死后诊断中的应用价值。方法 应用免疫组化LSAB法，对人心肌梗死心肌肌钙蛋白T的脱失情况观察。结果 人梗死心肌肌钙蛋白T有明显脱失，通过计算机图像分析显示，人梗死心肌肌钙蛋白T的脱失面积与正常人心肌之间存在显著性差异(P＜0.01)。结论 心肌肌钙蛋白T(CTnT)可能成为人心肌梗死的诊断指标之一。     

Institutional Change in Swiss Cantons: A Contribution to Describing and Explaining Institutional Reforms

Most accounts of Swiss federal institutions are unable to explain institutional change. In this contribution the authors trace change in Cantonal reforms and then use multivariate explanation models to account for institutional reforms such as New Public Management reforms. The multivariate analyses used rely on an “event count model” to explain the frequency of institutional reforms and a “binary response model” to explain why Cantons adopt New Public Management reforms. This research demonstrates that policy research as well as innovation and diffusion theories can contribute to explaining institutional reform.     

构建校园网多媒体信息点播(MOD)系统的探讨

构建校园网多媒体信息点播系统是建立先进的“数字化校园”环境的重要方面。文中提出了组建多媒体信息点播系统的操作步骤及方法。     

论WTO背景下我国税法改革基本原则的确立

根据世贸组织的规则和我国经济发展的实际,应尽快修改和完善税法体系。税收法定主义原则、税收公平主义原则、税收效率、协调性、渐进性原则应确立为我国税法改革的基本原则。     

中毒死者体内毒鼠强的GC/MS-SIM分析

一 1 9岁男子赌气吞服一白色粉末 ,1 0min后口鼻流血、抽搐 ,迅速送医院 ,经催吐、透析等方法治疗 5天后死亡。根据中毒症状和经验怀疑为毒鼠强中毒。用GC/NPD检验肝中毒鼠强有干扰。改用GC/MS -SIM法分析 ,心血、肝和胃组织中全部检出毒鼠强 ,浓度分别为 0 5 1 μg .mL- 1、0 4 5 μg .g- 1和 0 33μg .g- 1,和文献结果在同一水平。GC/MS -SIM对复杂检材中低含量毒鼠强测定具有更高的选择性 ,也具有较高的灵敏度 ,同时进一步证实毒鼠强排泄较慢 ,经过较长时间后在体内分布比较均匀     

论警检关系的基本特征与发展趋势

尽管世界上几个主要国家的警检关系存在三大模式的分野,但各国警察机关和检察机关之间既存在形式上的分工关系,又具有实质上的合作关系,只不过三大模式的侧重点有所不同而已。大致说来,英美模式的侧重点在审判程序,而大陆模式、中俄模式的侧重点在审前程序。但从一些国家近年来的司法改革看,三大模式在相互吸收的基础上,警检关系开始出现紧密化的发展趋势。     

国际金融市场研究方法的逻辑演变——有效市场假说面临的挑战

有效市场假说作为金融经济学的理论基石,无疑是我们研究国际金融市场的重要方法和依据。许多其他得到广泛 应用的金融投资理论皆以此为基础。历经30年的发展,有效市场理论在不断得到支持的同时,也面临了许多新的市场理论的挑 战。混沌理论和分形市场理论,特别是对于市场异象做出新解释的行为金融学,都从不同角度对传统有效市场理论进行了突破。这 不仅为研究国际金融市场提供了新的思路和方法,也对金融市场的决策者和生产者有着巨大的现实意义。