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Wenk RE 《Journal of forensic sciences》2011,56(Z1):S243-S246
Abstract: Relationship testing laboratories provide genetic evidence to support or refute claims of kinship between U.S. citizen petitioners and potential immigrant beneficiaries. One female beneficiary presented a male amelogenin type and alleles at 15 autosomal loci that were identical to an alleged brother’s. Laboratory records showed that her alleged father had petitioned to have 15 children emigrate from Ghana. The petitioner’s 15 paternity indices exceeded 105, but the children shared only four short tandem repeat (STR) profiles, suggesting fraudulent reuse of genotypes in this alleged pedigree (AP). To determine the extent of this “genotype recycling,” I examined the laboratory’s 555 APs from Ghana and 532 control APs from Nigeria. Seventeen Ghanaian APs (3.1%) but no Nigerian APs showed genotype recycling. Of 90 tested people in the 17 APs, 56 shared identical STR profiles with others in their AP. Of these 56 people, 10 were petitioners with unexpectedly high parentage indices. Seven of 56 had amelogenin types that disagreed with their declared genders. Database searches for identical multilocus genotypes in allegedly different people would best detect this fraud. 相似文献
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Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits 总被引:1,自引:0,他引:1
Hill CR Kline MC Mulero JJ Lagacé RE Chang CW Hennessy LK Butler JM 《Journal of forensic sciences》2007,52(4):870-873
The AmpFlSTR MiniFiler polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler and Identifiler STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift. 相似文献
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This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic casework, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification conditions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA. 相似文献
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自2002年党的十六大以来的10年间,以胡锦涛为总书记的党中央领导集体率领我们党以邓小平理论和"三个代表"重要思想为指导,不断推进理论创新,创建了科学发展观等重大战略思想理论,丰富发展了中国特色社会主义理论体系,开辟了马克思主义中国化最新境界,是马克思主义中国化最新理论成果,对新世纪新阶段的中国特色社会主义事业的发展具有重要的理论价值和指导意义。 相似文献
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司法警察支队长人格特征调查分析 总被引:2,自引:0,他引:2
采用卡特尔16项人格因素问卷(16PF)对202名司法警察支队长作测量,结果显示:司法警察支队长的人格特征较为优良;心理健康的平均得分高于常模均值;专业有成就的平均得分高于常模均值,其中得高分的占10.8%;创造力高分占12.4%;新环境成长能力平均得分略低于常模均值,成长能力高分占6.2%。这些结果表明,司法警察支队长总体的心理素质较为良好。 相似文献
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