新Y-STR基因座DYS709在汉族人群中的遗传多态性调查

目的筛选新的Y-STR基因座,调查其在汉族人群中的等位基因频率分布,评价其在法医学及其它方面的应用价值。方法在Y染色体基因组DNA中查找候选基因座,在重复顺序两端设计引物,PCR扩增后用银染法显示结果。结果一个重复单位为CTTT的Y-STR基因座DYS709被发现。在102例汉族无关男性个体血样中共检出了7个等位基因。基因多样性为0.7063,个人识别能力(PD)和非父排除率(PE)均为0.7063。结论新筛选到的DYS709具有较高的遗传多态性,在法医学及人类遗传学方面具有应用价值。     

气相色谱仪双毛细管柱双检测器技术在毒物检测中的应用

目的利用双毛细管柱双检测器技术建立常见毒物的检验方法,并对其进行定性分析。方法样品进样后通过VSOS装置进入两根不同极性的毛细管柱,经分离后到达两个不同选择性的检测器。结果利用VSOS装置可对毒物进行较好的分离、定性。结论该方法可对常见毒物进行准确定性。     

区域经济一体化对GATT/WTO体制的冲击和影响

GATT/WTO的制度缺陷通过区域经济一体化在形式和内容的发展更加明显,由于缺少权威的组织机构、无条件和自动适用的最惠国待遇原则以及受谈判方数目的影响,两者出现了此消彼长的趋势;GATT/WTO在规范区域经济一体化中存在着实体上、程序上和规范经济事物中的附加困难;区域经济一体化发展对国际经济法的贡献包括:区域经济一体化的发展在各主权国家中继续强化了国际经济法的普遍原则,扩展了国际经济法调整的领域,改进了调整的方式和效果,同时也为国际经济法整合区域经济组织与多边贸易体系产生了结构性动力。     

Gc亚型检测的复合MS-PCR法及其应用

目的探讨建立Gc亚型检测的复合MS-PCR法及其应用价值。方法根据Gc基因中的2处点突变,设计2对片段相差5bp的等位基因特异性引物和1条公共引物进行复合MS-PCR,分析Gc多态性,并调查武汉地区218例汉族无关个体Gc多态性和鉴定10例亲子关系。结果复合MS-PCR检测的Gc基因型,与AmpliTypePM试剂盒的分型结果一致;武汉地区汉族人群Gc基因的3个常见等位基因Gc1F、Gc1S、Gc2的基因频率分别为0.4816、0.2592、0.2592,观察杂合度(Hobs)、期望杂合度(Hexp)、多态性信息含量(PIC)、个人识别能力(DP)、非父排除率(PE)分别为0.6193、0.6359、0.6253、0.7974、0.3480,基因型分布符合Hardy-Weinberg平衡;真三联体和非真三联体亲子鉴定各5例,前者不排除父子关系,与常规STR分型一致,后者经Gc-MS-PCR分型排除2例。结论建立复合MS-PCR法检测Gc亚型在法医物证鉴定中有实用价值。     

8例利多卡因过敏性休克死的血药浓度分析

目的研究利多卡因过敏性休克死者利多卡因血药浓度及其与死因的关系。方法采用高效液相色谱法(HPLC),分别对使用利多卡因麻醉术中各项指标均正常的8例术中因利多卡因过敏性休克死者的血液和11例顺利完成手术患者的血液进行利多卡因浓度的检测,对比分析两者结果。结果 8例死者利多卡因血药浓度(1．61mg± 0．45mg／L)低于11例正常患者利多卡因血药浓度(2．44mg±0．47mg／L)。结论利多卡因过敏性休克死者血药浓度在正常值范围内,与发生过敏性休克致死无关。     

从非营利组织看事业单位的改革

中国在推进事业单位改革的过程中开始提出和使用非营利组织的概念,正确看待非营利组织现状和在中国公益事业中的作用,让事业单位和民间非营利组织平等发展,甚至相互竞争来促进事业单位提高服务意识和服务质量。使公益性,非营利性得到真正体现,并让改革的成果惠及全体人民。     

SPECIAL NEEDS CHILDREN IN FAMILY COURT CASES

This article presents a conceptual framework for describing cases involving children with special needs that are increasingly being seen in family court. Three categories that represent the most common of such cases include (1) acute, life-threatening medical conditions; (2) chronic developmental disorders; and (3) psychological and behavioral syndromes. After detailing the nature of the disabilities in each category, perspectives from the bench are offered with specific recommendations and general strategies for effective case management. This is followed by a proposal of an innovative conceptual model—the Individualized Parenting Plan (IPP)—that organizes and integrates nine domains essential for a comprehensive parenting plan for special needs children. The article concludes with a list of sixteen strategic guidelines for servicing the families of these special needs children, in their best interests.     

Strengthening Green Global Governance in a Disparate World SocietyWould a World Environment Organisation Benefit the South?

The debate on the advantages and disadvantages of upgrading the United Nations Environment Programme to a 'world environment organisation' (WEO) has gained momentum in both academe and politics. This article contends that a WEO would further the interests especially of developing countries, because it would provide them, first, a high-level forum to unite their individual bargaining power against the major industrialised countries. Second, a WEO would assist Southern efforts to garner international support for environmental programmes in regions and sectors that are increasingly bypassed by economic globalisation. Third, a WEO would create a locus to politically institutionalise the influence of non-governmental lobbyists in a way that increases the balance of opinions and perspectives. The article then addresses special challenges and caveats for developing countries in the upcoming negotiation process, in particular whether an upgraded UNEP would address only 'global' issues, thus absolving the international community from assisting developing countries in mitigating the more pressing local environmental issues in the South; whether the new body would have powerful sanctioning mechanisms, which might disproportionally affect developing countries; and what decision-making procedures the new body would have.     

英国法院对中国《海商法》和《对外贸易法》的适用和评价

通过介绍两个与中国法律或中国法院判决有关的案例,对中国法在外国的适用以及外国法院尤其是英国法院如何看待中国法院的判决进行了介绍和评析。