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目的建立测定血液中美西律(mexiletine)的液相色谱-串联质谱联用法(LC-MS/MS)。方法采用简便的乙腈蛋白沉淀法对血液进行预处理,应用Allure PFP Propyl液相柱分离,用电喷雾正离子模式离子化,多反应监测模式对美西律进行分析。结果美西律与内标纳洛酮分离良好,在0.02~10.00μg/mL内线性关系良好,相关系数为0.9999,回归方程为y=0.0283x-0.0151,日内与日间精密度的RSD均小于15%,最低检测限为0.01μg/mL。结论建立的LC-MS/MS方法简单、灵敏、可靠,可同时适用于美西律临床药物监测和法医毒物分析的需要。 相似文献
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Swider C Maguire K Rickenbach M Montgomery M Ducote MJ Marhefka CA 《Journal of forensic sciences》2012,57(4):923-931
Following the September 11, 2001 terrorist attacks, letters containing Bacillus anthracis were distributed through the United States postal system killing five people. A complex forensic investigation commenced to identify the perpetrator of these mailings. A novel liquid chromatography/mass spectrometry protocol for the qualitative detection of trace levels of meglumine and diatrizoate in dried spore preparations of B. anthracis was developed. Meglumine and diatrizoate are components of radiographic imaging products that have been used to purify bacterial spores. Two separate chromatographic assays using multiple mass spectrometric analyses were developed for the detection of meglumine and diatrizoate. The assays achieved limits of detection for meglumine and diatrizoate of 1.00 and 10.0 ng/mL, respectively. Bacillus cereus T strain spores were effectively used as a surrogate for B. anthracis spores during method development and validation. This protocol was successfully applied to limited evidentiary B. anthracis spore material, providing probative information to the investigators. 相似文献
146.
There is reason to suspect that lower levels of exposure to criminogenic peer‐based risks help explain why immigrant youth are less involved in crime and violence. However, it also is possible that if and when they do encounter these risks, immigrant youth are more vulnerable to them than are native‐born youth. Drawing from literature on the adaptation experiences of immigrant adolescents, we hypothesize that immigrant youth will be relatively more susceptible to the effects of both 1) exposure to deviant peers and 2) unstructured and unsupervised socializing with peers when compared with their nonimmigrant counterparts. Using a sample of approximately 1,800 adolescents from the Project on Human Development in Chicago Neighborhoods (PHDCN) study, we find support for our first hypothesis but not the second. Specifically, in both cross‐sectional and longitudinal models, we find that exposure to deviant peers has a greater impact on violence among immigrant youth than it does for native‐born youth. Furthermore, this pattern of results is supported with supplemental, sensitivity analysis using the AddHealth data. In contrast, there are no statistically significant differences across immigrant generation status with regard to the effect of informal socializing with peers on violence. 相似文献
147.
This paper discusses the controversy surrounding the Data Retention Directive with an emphasis on the 2011 decision of the Cyprus Supreme Court which has annulled several district court orders that allowed the police access to telecommunications data relating to certain persons relevant to criminal investigations. The annulment has been on the ground that the legal provisions upon which the orders have been issued are unconstitutional. It will suggest that the decision does not entail a direct rejection of the EU Data Retention Directive and that in any event, Cyprus is not a Member State resisting the particular measure. This is because the legal provisions are deemed unconstitutional, though part of the law that has transposed the relevant Directive into national law are provisions that go beyond what the EU legislator intended to regulate through that Directive. Still, the particular Directive sits rather uneasily within the ‘human rights’ regime, in particular the one governing the individual right of privacy. 相似文献
148.
The EU and the United States have implemented data breach notification rules that cover the health sectors. Nevertheless, data breach incidents involving medical data continue to rise, especially in the US and the UK. The HITECH Act, Pub. L. 111-5 Title XIII is the first federal health breach notification law in the US to be characterized by less government intrusions, while the revised EU Privacy Directive, 2009/136/EC calls for tougher privacy protection for data held by electronic communication providers. While the EU law sets a global de facto standard, the law remains toothless without strong enforcement mechanisms. 相似文献
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目的建立血液中硫化氢的气相色谱质谱联用分析方法。方法取心血3mL-5mL,置于20mL顶空瓶中,加入1g氯化钠,加3mL-5mL蒸馏水,加入2mol/L盐酸1mL,加盖密封,混匀后于80℃水浴中加热20min。取液上气体0.5mL进样分析。结果在中毒死亡者的心血中检出硫化氢,保留时间参考值为3.6min。结论该方法可用于刑事案件中硫化氢的快速分析。 相似文献