Differentiation of vaginal cells from epidermal cells using morphological and autofluorescence properties: Implications for sexual assault casework involving digital penetration

This work explores morphological and autofluorescence differences between vaginal and epidermal cells detectable through Imaging Flow Cytometry (IFC), a non-destructive, high-throughput technique. These differences were used to build a predictive framework for classifying unknown cells as originating from vaginal or epidermal tissue, which was tested on hand swabbings with and without digital penetration. Many more cells possessing a vaginal signature (median posterior probability ≥0.90) were detected in digital penetration samples than control hand swabbings. Minimum interpretation thresholds were developed to minimize/eliminate false positives; these thresholds were also effective when screening licked hands, indicating the potential utility of this method for a variety of biological mixture types and depositional events relevant to forensic casework.     

A Monte Carlo simulation and sensitivity cost benefit analysis for use of nylon 4N6FLOQSwabs®

Forensic genetic laboratories are challenged with implementing innovation even if the benefits to operational performance are well demonstrated often because of internal budget constraints. A prospective cost–benefit analysis (CBA) could support justification for an increased budget by effectively demonstrating in a system-based approach the relatively small cost of increasing a laboratory budget can substantially reduce costs to society (both qualitatively and monetarily). A Monte Carlo simulation and sensitivity CBA was performed using a more expensive swab (i.e., nylon 4N6FLOQSwabs®) compared with a less costly cotton swab. Ranges of input values and tangible and intangible benefits were considered. The outcome is that the relatively small increased cost of using a nylon swab pales compared with the potential tangible and intangible benefits to the overall system. This approach provides a sounder basis for requesting additional funds to support implementation of technologies and better approximates realistic situations while accommodating uncertainty of input values.     

Review on female sexual offenders: findings about profile and personality

Introduction

Although in the past sexual abuse was perceived as an issue connected only with males the contemporary literature is placing increasing emphasis on the role of female sexual perpetrators. There is still disagreement about the definition of sexual abuse, the frequency that it occurs and the characteristics of the women that are sexual abusers.

Methods

Thorough research of the main databases (MEDLINE and PsycInfo), for case reviews and studies along with restriction on European and North American literature, is due to perceived culture differences. Further investigation for relevant studies through web search engines such as Google, locates agencies and organizations that are interested and connected to sexual abuse issues.

Results

Distinction between sexual offense and sexual abuse has as a result difference in the characteristics of female sexual perpetrators. They are mainly young (age up to 36 years old), friends or relatives of the victim, using more persuasion and psychological coercion and legally charged in a lesser extent compared with male abusers. However the psychological consequences for the victim can be more severe.

Conclusion

A unanimous view of what is female sexual abuse is difficult to reach. Often it is under reported, unrecognized or considered ethically more acceptable than male abuse. It is also connected with an increased self-report of history of sexual abuse of the perpetrators. A typology of female sexual abusers should be developed. Treatments focusing on different psychological interventions along with prevention and public awareness can be a powerful tool in reduction of sexual abuse perpetrated by females.     

Young people who sexually abuse: the Scottish context

This paper describes the policy and legal context in Scotland in respect of alleged young sexual abusers and considers the challenges encountered by decision-makers, particularly reporters operating in the Scottish children's hearings system. These include the nature of the problem, resources, child witnesses and corroboration. It is argued that reporters, in considering whether or not to refer an alleged young sexual abuser to a children's hearing or to take no action, encounter influences in both directions, but on balance there appear to be more obstacles in the way of a referral to a hearing.     

Development of a rapid, 96-well alkaline based differential DNA extraction method for sexual assault evidence

We present a rapid alkaline lysis procedure for the extraction of DNA from sexual assault evidence that generates purified sperm fraction extracts that yield STR typing results similar to those obtained from the traditional organic/dithiothreitol differential extraction. Specifically, a sodium hydroxide based differential extraction method has been developed in a single-tube format and further optimized in a 96-well format. The method yields purified extracts from a small sample set (∼2-6 swabs) in approximately 2 h and from a larger sample set (up to 96 swabs) in approximately 4 h. While conventional differential extraction methods require vigorous sample manipulation to remove the spermatozoa from the substrate, the method described here exploits the propensity of sperm to adhere to a substrate and does not require any manipulation of the substrate after it is sampled. For swabs, sample handling is minimized by employing a process where the tip of the swab, including the shaft, is transferred to the appropriate vessel eliminating the need for potentially hazardous scalpels to separate the swab material from the shaft. The absence of multiple handling steps allows the process to be semi-automated, however the procedure as described here does not require use of a robotic system. This method may provide forensic laboratories a cost-effective tool for the eradication of backlogs of sexual assault evidence, and more timely service to their client agencies. In addition, we have demonstrated that a modification of the procedure can be used to retrieve residual sperm-cell DNA from previously extracted swabs.     

Semantic and Episodic Autobiographical Memory Recall for Memories Not Directly Associated with Childhood Sexual Abuse

A pattern of overgeneral autobiographical memory recall for memories not directly associated with the abuse has been found in previous research. However, studies with a non psychiatric population are limited. In a non psychiatric student population sample, this study will use two methods of accessing autobiographical memory, The Children's Autobiographical Memory Inventory (CAMI) and the Autobiographical Memory Task (AMT). Contrary to the predictions, this study did not find that episodic recall to the AMT was less specific and episodic recall to the CAMI contained greater detail for both the abuse group and the trauma control group compared to a no trauma control group. Significant differences were found between groups on personal semantic recall with the CSA group reporting significantly lower semantic recall than the two control groups. These findings are relevant to therapeutic interventions as well as having potential implications for interviewing and obtaining statements to be used as evidence.     

亚太妇女跨国迁移及其性产业与公共治理——菲律宾NGO的社会行动探析

20世纪90年代以来,亚太地区的人口迁移与性产业相互推动,促使越来越多的女性移民卷入性产业。如今,性产业的蔓延与繁荣已经成为亚太地区社会经济发展面临的一个严重问题,引起各国政府与相关非政府组织的极大关注。因此,治理性产业的法律与公共政策也成为亚太地区国家与公民社会讨论的重要内容。本文以菲律宾妇女海外性工作者与菲律宾NGO的社会行动为例,探讨发展中国家的妇女移民在跨国迁移中的角色、地位与社会、性别危机,以及公民社会参与性产业的社会治理行动与策略。     

Routine Activity Theory and Sexual Deviance Among Male College Students

Much research on sexual deviance on university and college campuses is limited by its narrow focus on group affiliation. In this study, it is argued that prior deviance and opportunity serve as more robust predictor variables of sexual deviance. Using a sample of 304 male college students from four universities within the Ohio Valley Conference, this paper utilizes Analytic moment structures (AMOS) analyses to examine the relationship between prior deviance and sexual deviance. Results support the assumption that sexual aggression is significantly (β=.17) mediated or accounted for by opportunity. Overall, the relationships among variables lend support to the argument that alternative explanations of sexual deviance may provide a better understanding of such a complex societal problem.

论平滑肌是肝藏调节性生理的解剖基础

论证了肝藏与生殖的密切关系,基于肝藏的生理解剖基础是平滑肌系统这一认识,对"肝行肾气"、"女子以肝为先天"两个中医学理论进行了讨论,指出肝藏在生殖方面所发挥作用的生理解剖基础是生殖系统的平滑肌.肝藏与平滑肌关系的理论在生殖方面得到了逻辑统一的论证.