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11.
目的调查DYS391等24个Y-STR基因座在南京汉族人群的遗传多态性,考察其在法医学中的应用价值。方法应用AGCU Y-PLUS(24)PCR试剂盒对南京580名汉族无关男性个体进行Y-STR基因座扩增检测分型,用软件计算24个基因座的基因频率等群体遗传学参数,并与湖北、辽宁、广东、北京、成都汉族人群数据进行比较。结果南京580名汉族无关男性个体在24个Y-STR基因座共发现580种单倍型,各基因座的基因多样性(GD)为0.294 6~0.939 8,单倍型多样性(HD)为0.983 7。六地人群GD值的差异有统计学意义。结论 DYS391等24个Y-STR基因座在南京地区有法医学价值,可用于案件检验及家系排查。 相似文献
12.
单亲案亲权鉴定结果判定策略 总被引:4,自引:0,他引:4
目的探讨用STR基因座进行单亲鉴定出现矛盾基因座时下结论的策略。方法根据基因频率和遗传规律,推导单亲案亲权鉴定时的非父排除率。根据平均单亲非父排除率和平均突变率,用二项分布公式分别计算出现不同数目矛盾基因座时真父和假父的概率和似然率(亲权指数)。结果对STR共显性基因座,其单亲非父排除率的计算公式为:PEM=∑i=n1pi2(1-pi)2 ∑i相似文献
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14.
壮族人群3个STR基因座基因频率分布及其法医学应用 总被引:6,自引:0,他引:6
研究3个STR基因座(D21S11、HumFGA、D19S253)在广西壮族人群中的基因频率分布及其在实际检案中的应用价值。以自制等位基因Ladder样品作为标准对照,用PCR结合PAGE技术对3个STR基因座的扩增产物进行分型。结果显示:D21S11基因座有14个等位基因,有44个基因型;HumFGA基因座有15个等位基因,40个基因型;D195253基因座有9个等位基因,23个基因型。经检验,3个STR基因座基因型分布均符合Hardy-Weinberg平衡,累计个体识别力(DP)为0.9995。3个STR基因座在壮族人群属高识别力遗传标记系统,在法医学个体识别及亲权鉴定方面有重要价值。 相似文献
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Walther Parson Harald Niedersttter Alexandra Lindinger Peter Gill On behalf of the ENFSI DNA Working Group 《Forensic Science International: Genetics Supplement Series》2008,2(3):238-242
The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA. 相似文献
17.
C. Robino S. Inturri A. Piccinini C. Torre 《Forensic Science International: Genetics Supplement Series》2008,1(1):160-161
Typing of X-chromosomal short tandem repeat (STR) loci in a deficiency paternity case revealed a single Mendelian incompatibility between a female child and her putative grandmother, consisting of an opposite homozygosity at locus DXS8378. The presence of a null allele due to a primer binding site mutation on the child's paternally inherited X chromosome was confirmed by amplification with newly designed DXS8378 external primers. Sequencing analysis showed a point mutation (C > T transition at position 168, according to GenBank accession G08098) in the binding site of the original DXS8378 reverse primer. 相似文献
18.
D.J. French R.L. Howard N. Gale T. Brown D.G. McDowell P.G. Debenham 《Forensic Science International: Genetics Supplement Series》2008,2(4):333-339
Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR® SGM Plus® system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1 h. 相似文献
19.
Masaki Hashiyada Yukio Itakura 《Forensic Science International: Genetics Supplement Series》2008,1(1):150-152
Eight X-chromosomal short tandem repeat (X-STR) markers were analyzed in 258 unrelated Japanese (144 males and 114 females) using Mentype® Argus X-8 PCR Amplification Kit (Biotype AG) which contains DXS7132, DXS7423, DXS8378, DXS10074, DXS10101, DXS10134, DXS10135 and HPRTB. The DXS10135 locus proved to be highly polymorphic marker (PIC: 0.945) and the DXS7423 showed the lowest value (PIC: 0.453). The exact test for genotype distribution showed no significant deviation from the Hardy-Weinberg equilibrium. 相似文献
20.
Karlheinz Thiele Steffi Löffler Franziska Günthner Jeanett Edelmann 《Forensic Science International: Genetics Supplement Series》2008,1(1):167-169
The investigation of the X-linked DNA markers are well established in the forensic routine case work. We studied an Ewe population sample from Ghana. The eight X-chromosomal STRs DXS10135, DXS8378, DXS7132, DXS10074, HPRTB, DXS10101, DXS10134 and DXS7423 were analyzed in 182 Ewe individuals (108 females and 74 males) from the region of Sogakofe (Ghana). Allele frequencies and statistical parameter as well as comparison with known data from Germans and with data from an Amharic population (Ethiopia) are presented. 相似文献