人线粒体DNA序列分析在法医学中的应用研究及其进展

综述人线粒体DNA(m tDNA)序列分析在法医学种属鉴别、个体识别,以及个体年龄推断中的应用研究及其进展,展望对m tDNA异质性的研究及建立人m tDNA数据库,并具有重要的法医学实践意义。     

小鼠骨骼肌细胞核DNA降解与死亡时间的关系

目的　监测小鼠死后骨骼肌核内DNA降解的情况 ,探讨死后细胞核DNA降解的一般规律。方法　建立小鼠死亡模型 ,在死后 72h内 ,以 12h的间隔取骨骼肌样本进行单细胞凝胶电泳 ,在荧光显微镜下测量彗星图像 ,并作统计学分析。结果　机体死后 ,骨骼肌细胞在电泳图像上出现了明显的彗星形拖尾 ,L/W比值随死亡时间而逐渐增大 ,二者呈一定的线性关系。结论　单细胞凝胶电泳技术可以应用于早期死亡时间推断。     

谈文件检验在侦破匿名信敲诈案件中的应用

近年来,全国各地匿名信敲诈案件大量发生。作案人使用伪造的或非本人的身份证到银行申领信用卡,书写或打印匿名信件对知名人士或各类企业、各级党政机关人员进行敲诈,严重扰乱和影响了社会正常生活秩序,甚至严重危害社会公共安全。文件检验是侦破此类案件的重要手段和突破口。     

Bounding the number of contributors to mixed DNA stains

We derive a simple inequality for the probability of observing a given DNA profile when assuming a fixed number of unknown persons have contributed to the mixed stain. We then show how this inequality can be used to obtain an upper bound for the number of unknown contributors needed to be considered.     

DNA指纹的个体特异性和细胞稳定性研究

人类基因组的许多高度可变区域能够与小卫星探针杂交,而同时被检测出来,小卫星探针由串联重复的"核心"顺序构成.采用Southern印迹杂交获得的DNA指纹图包含多条高度可变的DNA片段杂交带,这些带主体细胞和生殖细胞中都表现出稳定性,而且安全具有个体特异性.本文用小卫星探针33.15,调查了北京地区的60名无关个体,     

The first woman in the process of book production in Livonia: the case of Ursula Krüger and Daniel Hermann

I present the first case from the history of early modern Livonia, when a woman participated in the editing and publication of a printed book. Ursula Krüger, wife of Prussian–Livonian humanist Daniel Hermann, illustrates the wider perspective of non-aristocratic women’s activities involving book production in the geographical periphery of humanist culture, Riga, rather than in the heart of European humanism. Her story also illustrates the ability of women to collaborate as an editor, both as a learned woman who could compose Latin dedications and a businesswoman who could financially and organizationally engage in publishing books.     

Touch DNA collection from improvised explosive devices: A comprehensive study of swabs and moistening agents

Improvised explosive devices (IEDs) are used in devastating terrorist attacks worldwide and daily in Thailand. Touch DNA deposited during IED assembly are subjected to intense heat and pressure, resulting in rare events of usable DNA profiles obtained from real casework. No study has simultaneously evaluated both swab brands and moistening agents for touch DNA collection from substrates encountered in IED evidence. In this study, we investigated the effects of swab brands and moistening agents on DNA collection from adhesive tape, a common IED substrate. A full factorial design using four cotton swab brands (two forensic and two medical cotton swabs) and six moistening agents (DNA-free water, phosphate-buffered saline, ethanol, sodium dodecyl sulfate, isopropanol, and lysis buffer) was employed (24 total combinations). Using buffy coats, we found that DNA recovery depended on both swab brands and moistening agents (p < 0.05). The optimal method recovered significantly higher DNA amount from real IED cases compared to the standard Royal Thai Police method. Percentages of high partial profiles also increased. Our results changed the standard operating protocol of the Thai police. Other commonly found substrates from IED cases are being investigated to maximize the evidential value obtained from touch DNA.     

贵州民族体育遗存——独竹漂运动的历史和未来

在对独竹漂运动的起源和发展进行追溯的基础上,预测了其未来走向。独竹漂运动起源于明朝洪武年间的"水运皇木"过程中,其历经民间发展、自然传承,惨遭禁止,几乎绝传,逐渐恢复,喜获新生三个历史阶段。今后独竹漂运动将朝着竞速水平不断提高,技艺表演日益发展;队伍规模逐渐扩大,社会影响不断提高;经济价值不断提升,产业发展日益显现;研究水平逐步提高,理论成果不断丰富的发展趋势。     

土地革命时期毛泽东信念观探析——重温《井冈山的斗争》与《星星之火， 可以燎原》

1927年大革命失败后,中国革命进入了十年土地革命时期。期间党与红军内部出现了"右"倾悲观思潮,并几度提出"红旗到底打得多久"的疑问。对此,毛泽东在《井冈山的斗争》与《星星之火,可以燎原》等著作中给予了科学回答,形成了毛泽东信念观:坚定信念创建农村革命根据地;坚信红色政权的发生、存在与发展既是可能的,也是必然的;坚信星火必燎原,中国革命高潮必将到来。毛泽东信念观的形成是大革命失败后时局变化的产物,是实行正确政策的必然结果。     

Estimating Individual Contributions to Complex DNA SNP Mixtures.,

High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures.