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941.
目的研究色氨酸羟化酶(tryptophan hydroxylase,TPH)基因座-T457C位点在中国北方汉族群体中的遗传多态性及其法医学应用价值。方法应用PCR-RFLP、聚丙烯酰胺凝胶电泳及银染技术,检测180例北方汉族无关个体TPH基因座-T457C位点的遗传多态性。结果TPH基因座-T457C位点在中国北方汉族群体中,其个体识别能力(DP)为0.624,非父排除率(PE)为0.187,与法国人群基因频率比较差异显著(P<0.05)。结论TPH基因座-T457C位点在法医学中有重要应用价值,其遗传多态性可能存在民族或地域差异。 相似文献
942.
Masaru Asari Ph.D. Tomohiro Omura Ph.D. Chikatoshi Maseda Ph.D. Kazuo Matsubara Ph.D. Hiroshi Shiono M.D. Ph.D. Keiko Shimizu M.D. Ph.D. 《Journal of forensic sciences》2010,55(6):1576-1581
Abstract: We developed a new method for forensic ABO genotyping based on a universal reporter primer (URP) system. This allows for the simultaneous detection of six single nucleotide polymorphism (SNP) sites in the ABO gene (nucleotide positions 261, 297, 526, 703, 796, and 803). This URP system provides obvious peaks, ranging from 82 to 151 bp in length. ABO genotypes were classified and successfully genotyped by our method, including minor alleles that may cause a discrepancy between the genetic data and serological phenotypes. Full profiles were identified using as little as 0.1 ng (0.05 ng/reaction) of standard K562 and 9947A DNA. Moreover, the success rate of genotyping from a URP system was much higher than that from a conventional primer extension method in degraded DNA. This method enables simple and rapid detection of multiple SNP sites on human ABO genes and is highly specific and sensitive when using limited and degraded DNA. 相似文献
943.
目的建立血浆中丁丙诺啡液相色谱/质谱(LC/MS)分析方法。方法在含有丁丙诺啡的血浆中,加入内标奋乃静,加pH10.8缓冲溶液,用401有机担体作吸附剂、三氯甲烷作洗脱剂固相萃取,N2挥干,用50μL流动相定容后进行LC/MS分析。色谱条件:Thermo Hypersil-HyPURITY C18(150×2.1mm,5μm),柱温:40℃,流动相:10mmol/LNH4AC(pH3.4)∶甲醇∶乙腈=36∶52∶12,流速:0.22mL/min。结果方法的线性范围为0.05~5.0μg/L(r=0.9998),定量限0.05μg/L,检出限0.01μg/L(S/N=3);3个浓度的质量控制样品(0.1μg/L,0.5μg/L,2.0μg/L)平均回收率分别为86.40%,92.72%,92.57%,RSD分别为4.51%,3.34%,2.09%。结论该方法操作简便、灵敏度高,可用于涉毒案件血浆中丁丙诺啡的分析。 相似文献
944.
Business Registers (BRs) are a very important information resource for investors, creditors, financial institutions and public authorities. The possibility to aggregate and interconnect these data at a European level could enhance the transparency of companies towards those actors and add a great deal of value to the raw Business Register data. The European BRITE project intended to provide adequate tools to meet these demands. BRITE will provide easier access and cross-border interoperability of Business Register data throughout Europe. On the other hand, the processing of BR data within the BRs and BRITE triggers several important European legislations such as the Data Protection Directive and the Directive on the re-use of public sector information. In this paper, the processing of BR data will be analysed from the perspective of both data protection and public sector information laws, analysing as well the relation between both regulations. Do these regulations strike an optimal balance between the interests of private data vendors to re-use BR data and enhance business transparency and the need to protect the personal data of natural persons? 相似文献
945.
陈仪 《甘肃行政学院学报》2009,(4):26-33
人大掌控政府预算审批权是民主政治的基本要求,也是预防腐败、增进民众信任、建设社会主义和谐社会的重要保障。但目前我国在人大代表人员组成、会议议期、议事规则、预算财政年度、初审程序、预算信息公开等方面的制度缺陷,直接限制了人大监督作用的发挥。同时,人大代表缺少对预算草案的修正权也间接妨碍了人大审批权的实际实施。欲激活人大的预算审批权,应进一步推进财政信息公开、赋予代表对预算案的修正权、调整预算年度、强化预算初审,并逐步完善我国的人民代表大会制度。 相似文献
946.
Anthony T. Quirk John M. Bellerby James F. Carter Fay A. Thomas Jenny C. Hill 《Science & justice》2009,49(2):87-93
A number of two-way radios, similar to those which have been employed to initiate Improvised Explosive Devices (IEDs), were acquired from a commercial supplier and grouped into four pairs. Samples of plastic material were collected from five distinct regions of each radio and analysed by Infrared and Raman spectroscopy to identify the nature of the material. One radio of each pair was then subjected to detonation with a commercially available plastic explosive. The combination of radio and explosive was considered to be representative of the components of an IED. Following detonation, fragments were recovered and, where possible, identified as specific sampling points of the radio.A combination of δ2H and δ13C stable isotopic analysis of material from each of the five sampling points was found to provide a pattern which was characteristic of a given radio and provided a means to associate pairs of radios. When few fragments were recovered, no positive association could be made between the fragments and the paired, undamaged radio. This was attributed, in part, to manufacturing variation in the radios. However, when three or more post-blast fragments were recovered it was possible to associate these with the paired, undamaged radio with a high degree of certainty. 相似文献
947.
Since the publication of analyses suggesting the significant impact on youth homicide of the Boston “pulling levers” intervention,
a series of studies of similar strategies have indicated promise in reducing homicide and gun assaults. One of these studies
was an assessment of a pulling levers strategy in Indianapolis, where trend analyses indicated a significant reduction in
homicide following the intervention, while six other similar Midwestern cities did not experience a significant decline in
homicide. We re-assess the results of the Indianapolis study by disaggregating the offenses into gang- and non-gang homicides.
Given that the pulling levers program focused on influencing gangs and networks of chronic offenders, the impact of the intervention
should be more apparent for gang homicides than for non-gang homicides. Alternatively, should the impact be similar for non-gang
homicides, then it is more likely that the downward trend would be caused by unmeasured external forces. Coefficient-difference
tests relying on estimates obtained from autoregressive integrated moving average (ARIMA) time–series models indicate that
gang homicides declined significantly more than did non-gang homicides following the Indianapolis intervention. These findings
suggest ‘something happened’ to gang homicides that did not happen to non-gang homicides, which adds further support that
the pulling levers initiative was the driving force behind the overall reduction in homicide in Indianapolis.
Nicholas Corsaro is an assistant professor in the Center for the Study of Crime, Delinquency, and Corrections at Southern Illinois University-Carbondale (SIUC). Prior to joining SIUC, he completed his Ph.D. at Michigan State University in 2007. Corsaro’s research interests include strategic approaches to reducing crime, ecological criminology, program evaluation, and quantitative statistical techniques. Recent articles have appeared in Victims and Offenders and Justice Quarterly. Edmund McGarrell is professor and director of the School of Criminal Justice at Michigan State University. McGarrell’s research interests are in communities and crime, with particular emphasis on the studying of problem solving responses to gun, gang, and drug market crime and violence. Recent articles have appeared in Justice Quarterly, Policing, Journal of Contemporary Criminal Justice, and the Journal of Criminal Justice. 相似文献
Nicholas CorsaroEmail: |
Nicholas Corsaro is an assistant professor in the Center for the Study of Crime, Delinquency, and Corrections at Southern Illinois University-Carbondale (SIUC). Prior to joining SIUC, he completed his Ph.D. at Michigan State University in 2007. Corsaro’s research interests include strategic approaches to reducing crime, ecological criminology, program evaluation, and quantitative statistical techniques. Recent articles have appeared in Victims and Offenders and Justice Quarterly. Edmund McGarrell is professor and director of the School of Criminal Justice at Michigan State University. McGarrell’s research interests are in communities and crime, with particular emphasis on the studying of problem solving responses to gun, gang, and drug market crime and violence. Recent articles have appeared in Justice Quarterly, Policing, Journal of Contemporary Criminal Justice, and the Journal of Criminal Justice. 相似文献
948.
比较法视野下的东亚法律人才养成制度改革 总被引:3,自引:0,他引:3
本文对20世纪末以来中国、日本、韩国借鉴美国法学院制度进行的法学教育改革进行了概要介绍,在此基础上,对三国继受美国法学教育模式的动因进行了分析,比较了中、日、韩三国在新法学教育制度设计上的差异,评价了改革的实践效果。作者认为,比较法的研究可以为中国法学教育改革提供更加广阔的国际视野,中国建构科学合理的法律人才养成制度,必须全面反映和评估对法律人才的需求,并在此基础上进行整体性改革才能确保改革目标的实现。 相似文献
949.
Antoinette A. Westen Anuska S. Matai Jeroen F.J. Laros Hugo C. Meiland Mandy Jasper Wiljo J.F. de Leeuw Peter de Knijff Titia Sijen 《Forensic Science International: Genetics Supplement Series》2009,3(4):233-241
For the analysis of degraded DNA in disaster victim identification (DVI) and criminal investigations, single nucleotide polymorphisms (SNPs) have been recognized as promising markers mainly because they can be analyzed in short sized amplicons. Most SNPs are bi-allelic and are thereby ineffective to detect mixtures, which may lead to incorrect genotyping. We developed an algorithm to find non-binary (i.e. tri-allelic or tetra-allelic) SNPs in the NCBI dbSNP database. We selected 31 potential tri-allelic SNPs with a minor allele frequency of at least 10%. The tri-allelic nature was confirmed for 15 SNPs residing on 14 different chromosomes. Multiplex SNaPshot™ assays were developed, and the allele frequencies of 16 SNPs were determined among 153 Dutch and 111 Netherlands Antilles reference samples. Using these multiplex SNP assays, the presence of a mixture of two DNA samples in a ratio up to 1:8 could be recognized reliably. Furthermore, we compared the genotyping efficiency of the tri-allelic SNP markers and short tandem repeat (STR) markers by analyzing artificially degraded DNA and DNA from 30 approximately 500-year-old bone and molar samples. In both types of degraded DNA samples, the larger sized STR amplicons failed to amplify whereas the tri-allelic SNP markers still provided valuable information. In conclusion, tri-allelic SNP markers are suited for the analysis of degraded DNA and enable the detection of a second DNA source in a sample. 相似文献
950.
This study aims to contribute to the analysis of the impact of alcohol consumption on road traffic safety in Slovenia and to evaluate the effectiveness of the changes in road traffic safety legislation concerning this issue within the period 1980–2006. For this purpose we have performed a retrospective analysis of 1913 autopsy reports on traffic casualties, with an emphasis on their blood alcohol concentration (BAC), mode of participation in traffic (drivers, pedestrians, etc.) and the impact of legislation changes. In addition, we have analysed the relations between BAC, the time and day of death and the gender of victims. Changes in the legislation were made in 1981, 1985, 1988, 1989, 1990, 1998 and 2005, yet only those made in 1981, 1990 and 1998 resulted in the increase of the actual fines. Within the observed period there was a substantial reduction in the average BAC in almost all of the categories of traffic participants (pedestrians: from 1.20 to 0.73; passengers: from 1.08 to 0.56; cyclists: from 1.11 to 0.56; motorcyclists: from 0.68 to 0.33) except in the category of drivers (negligible reduction from 0.81 to 0.76). Insufficient improvement in the latter category is extremely important, because many sober vulnerable traffic participants become victims of drunk drivers. Further concern arises from the finding that heavily drinking drivers are not responsive to penalty increases. We came to the conclusion that, in addition to raising fines and expanding penalty points, the introduction and effective enforcement of complex legislative measures together with wide community action are necessary to resolve DUI problems in Slovenia. 相似文献