盖塔病毒6K蛋白单克隆抗体的制备与鉴定

为用于猪群中盖塔病毒(Getah virus,GETV)的流行病学调查,并研究其6K蛋白功能,制备了该蛋白的单克隆抗体.将6K基因克隆至原核表达载体pCold-TF,并转化大肠杆菌BL21(DE3),进行IPTG低温诱导表达.将诱导的重组菌超声裂解,离心后收集上清,经过镍柱亲和和切胶纯化后,免疫BALB/c小鼠,取免疫...     

Electrical and thermal injuries in pig skin — Evaluated and compared by light microscopy

The morphology of pig skin after electrical (el) and thermal exposure was studied in order to find methods applicable for disclosing electrical torture.Biopsies from pig skin exposed 24 hours earlier to either heat or electrical current under general anesthesia were studied by light microscopy. The amount of energy used of either type ranged from about 6 to about 100 joule deposited on two circular areas of the skin measuring 12 mm in diameter.In sections stained with hematoxylin and eosin alterations were found in heat-influenced specimens following the administration of moderate and large doses of energy. Changes were observed in el-influenced specimens also after the administration of low amounts of energy, which did not lead to macroscopical changes in the skin. The two types of energy induced different patterns of alterations inside the epidermis. The most characteristic change following heat exposure was the presence of a granular or fibrillar eosinophilic cytoplasm and subepidermal clefts, while el-expoure often produced white homogeneous cytoplasm and shadowy nuclei (“white necrosis”) in the attached epidermis. The individual changes could be produced by both types of injury, but to a highly different degree. However, the alteration classified as “vesicular nuclei” was only observed in el-damaged skin. The dermis from the more severely injured skin of both groups showed a homogeneous appearance (“necrosis”). While the changes of epidermis and dermis in response to heat were diffusely distributed, the changes created by electrical injury were often present in segments. By electrical damage homogeneous areas with loss of stainability and shadowy nuclei were occasionally observed in sweat glands, hair sheaths and in vessel walls, structures which are suggested to serve as conductors for the electrical current. “Vesicular nuclei” were observed in similar structures.Some of the findings may be associated with differences in intensity of energy during exposure to heat and electricity. Furthermore, the existence of different biological patterns following the two types of exposure should be considered. The studies are being continued to elucidate these basic questions, and to study further the medical diagnostic potential of examining skin biopsies from tortured individuals.     

4种硅藻检验方法的比较研究

目的比较硝酸乙醇法、酶消化法、“破机罐”法和Soluene-350法在检验硅藻中各自的优缺点,为法医实际办案提供理论依据。方法取家兔肝、肾和骨髓3种组织各2g,分别用上述4种方法消化,比较4种不同方法分别将组织这些完全消化所需要的时间、消化能力、对硅藻的检出率以及对硅藻的破坏程度。结果消化时间以Solu-ene-350法耗时最长,其次是酶消化法,硝酸乙醇法和破机罐法耗时最短。4种方法消化能力的强弱依次是“破机罐”法、硝酸乙醇法、酶消化法、Soluene-350法。对梅尼小环藻(淡水)和桥弯藻(淡水),酶消化法的检出率最高,硝酸乙醇法其次,“破机罐”法和Soluene-350法最低。对舟形藻(海水),酶消化法的检出率高,其余3种方法几乎没检测到。扫描电镜观察:酶消化法对硅藻结构几乎没有破坏。其余3种方法对硅藻均有不同程度的破坏。结论从降低成本、提高检测效率角度,应选择“破机罐”法和硝酸乙醇法;从安全性和环保角度,或可疑水样为海水及硅藻密度较小时,应选择酶消化法;Soluene-350法因耗时长、成本高且硅藻检出率低,不适于在基层法医中推广。     

A note on the optimality of (even more) incomplete strict liability

This paper starts from the premise that liability is incomplete and establishes that firms may nevertheless invest excessively in care. This may justify a (further) reduction in the level of liability from a social standpoint, thereby arguing against seeking to approach full compensation as close as possible. In our framework, firms are liable under product liability, but also invest in care to prevent consumers’ switching to competitors. Affecting the partition of consumers by means of care-taking is not desirable from a social standpoint. Consequently, it may be optimal to reduce liability below a given level of incomplete compensation in order to adjust firms’ care incentives.     

Looking from South Africa to the World: A Story of Identity for our Times

The article is a consideration of the question of identity in South Africa, and also in a global context. Just as South Africa has looked to the world in order to understand its place, so too the world might look to South Africa to illuminate patterns less immediately visible elsewhere. Far from being unrepresentative in the apartheid period, South Africa was the ‘state of exception’ that incarnated and concentrated global realities; equally in the current era the reciprocal relations between the South African and the global evoke haunting concerns. The article begins with a consideration of the ‘classic’ generation of anti-apartheid activists, including figures such as Nelson Mandela and Bram Fischer, as they fashioned a new sense of South African identity. Yet it goes on to consider what happens when the classic period is over, and older definitions and oppositions are no longer available. Here the navigations of fiction, both in South Africa and elsewhere, become significant, and the article examines the work of writers from Gordimer, Coetzee and Ndebele, to Caryl Phillips and W. G. Sebald. It ends with a contemplation of the current period, nearly twenty years after the democratic transition in South Africa. In the era of the Marikana massacre and other pressing developments, both music and fiction open up some of the ambiguities and obligations. Drawing on Agamben, I suggest the intrinsic mutuality of the ‘home’ and the ‘foreign’ in establishing a more promising—and challenging—sense of belonging and identity both in South Africa and the world.     

The Leaderless Nexus: When Crime and Terror Converge

This article argues that the breakdown of hierarchical structures in illicit organizations is creating new opportunities for criminals and terrorists to collaborate. The rise of networked organizations has given greater independence to criminals and terrorists who previously answered to a clear chain of command. These members are now willing to engage in operations that before had been off-limits because the leadership believed the activity would hurt the organization's broader mandate. The result is that a “leaderless nexus” is beginning to emerge between criminals and terrorists. The phenomenon has far-reaching and dangerous implications for U.S. security, and should be thoughtfully considered as lawmakers debate homeland security reform.     

Evaluation of four digestive methods for extracting diatoms

Objective

To compare the merit of four digestive methods (nitric acid plus hydrogen peroxide, proteinase K, nitric acid in Disorganization Can and Soluene-350) for extracting diatoms in order to choose the best digestive method for the diagnosis of drowning.

Methods

Liver, kidney and bone marrow of rabbits were minced and then digested by four digestive methods separately with the following indices compared: (1) time demanded for complete digestion; (2) degree of digestion for different tissues; (3) the reclaiming ratio of diatoms; (4) the degree of digestive destruction to diatoms.

Results

For sufficiently digesting the same tissue, the demanded times for the different methods ranked from the longest to the shortest were as follows: Soluene-350, proteinase K, nitric acid plus hydrogen peroxide, nitric acid in Disorganization Can. Nitric acid in Disorganization Can method and nitric acid plus hydrogen peroxide method digested the tissues more thoroughly than proteinase K, than Soluene-350 methods. For Cyclotella and Cybella, proteinase K method reclaimed most diatoms and nitric acid plus hydrogen peroxide method reclaimed less, while nitric acid in Disorganization Can and Soluene-350 methods reclaimed the least. For Navicula, the majority of diatoms could be extracted using proteinase K method, but only a few diatoms with other three methods. Under scanning electron microscopy (SEM), the structure of diatoms remained almost perfect after digestion with proteinase K, but destroyed to some extent with other three methods.

Conclusion

This study demonstrated that different diatoms (in fresh or sea water) have different resistance to different digestive reagents. As far as the reliability and applicability of the diatom test is concerned, proteinase K method is of the best choice, nitric acid plus hydrogen peroxide can be its substitute. Soluene-350 cannot be used for extracting sea water diatoms.     

艺术中的直觉抽象——朗格、卡西尔的抽象论

一般认为,抽象和直觉是对立的。卡西尔认为,艺术抽象和科学抽象是互逆的:前者是一个不断具体化的夸张的过程,后者是一个不断凝缩的过程。在卡西尔学说的基础上,朗格把抽象和直觉统一起来,从艺术发生学的角度进一步研究后认为,直觉中蕴涵着逻辑经验,直觉是一切抽象的开始。艺术中的抽象是直觉抽象。尽管艺术离不开这种抽象,但抽象在艺术中仍居次要地位。     

人参注射液合缺血预处理对家兔心肌缺血再灌注损伤的保护作用

目的:观察人参注射液合缺血预处理对缺血再灌注家兔心肌的保护作用.方法:复制家兔心肌缺血再灌注模型,观察人参注射液、缺血预处理及人参注射液合缺血预处理对模型家兔心肌耗氧量和血浆中Na ,K -ATP酶含量的影响.结果:人参注射液、缺血预处理均能降低心肌耗氧量,升高血浆Na ,K -ATP酶含量,与单纯缺血再灌注组比较,差异有统计学意义(P<0.05),而人参注射液合缺血预处理作用最显著(P<0.05或P<0.01).结论:人参注射液能加强缺血预处理对心肌的保护作用.     

美韩非对称性同盟对朝核困局影响探析——以文在寅政府时期为例

朝核问题是冷战后东北亚地区的热点问题,一直影响着本地区的安全。并且,由于朝鲜半岛独特的地缘政治特性以及该地区的大国安全博弈,朝核问题的解决难度与日俱增。长期以来,韩国将美韩同盟视为朝核危机发酵期的安全保障,同时,朝鲜亦因美韩同盟的持续强化而极速推进核武力建设,最终导致朝核危局愈演愈烈。应当说,朝核危机与美韩同盟两者间已互为因变量。然而,美国作为世界唯一超级大国,其战略指向不仅是半岛无核化,更主要的战略意图是为其全球战略体系服务。因此,“美主韩从”性质的美韩同盟会使韩国本身的战略指向发生偏移,最终,非对称性质的美韩同盟可能会成为阻碍半岛和平繁荣的关键因素。