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41.
Brett A. Schweers Jennifer Old P.W. Boonlayangoor Karl A. Reich 《Forensic Science International: Genetics Supplement Series》2008,2(3):243-247
Human blood is the body fluid most commonly encountered at crime scenes, and blood detection may aid investigators in reconstructing what occurred during a crime. In addition, blood detection can help determine which items of evidence should be processed for DNA-STR testing. Unfortunately, many common substances can cause red-brown stains that resemble blood. Furthermore, many current human blood detection methods are presumptive and prone to false positive results. Here, the developmental validation of a new blood identification test, Rapid Stain Identification™-Blood (RSID™-Blood), is described. RSID™-Blood utilizes two anti-glycophorin A (red blood cell membrane specific protein) monoclonal antibodies in a lateral flow strip test format to detect human blood. We present evidence demonstrating that this test is accurate, reproducible, easy to use, and highly specific for human blood. Importantly, RSID™-Blood does not cross-react with ferret, skunk, or primate blood and exhibits no high-dose hook effect. Also, we describe studies on the sensitivity, body fluid specificity, and species specificity of RSID™-Blood. In addition, we show that the test can detect blood from a variety of forensic exhibits prior to processing for DNA-STR analysis. In conclusion, we suggest that RSID™-Blood is effective and useful for the detection of human blood on forensic exhibits, and offers improved blood detection when compared to other currently used methods. 相似文献
42.
目的:观察养肝益水颗粒对高血压早期肾损害患者尿清蛋白排泄率(urinary albumin ex-cretion ratio,UAER)、尿微量清蛋白(microalbuminuria,MA)、β2微球蛋白(β2-microglobulin,β2-MG)、α1微球蛋白(α1-microglobulin,α1-MG)、转铁蛋白(transferrin,TRF),免疫球蛋白G(immu-noglobulin G,IgGu)的影响.方法:选择80例符合中医辨证的高血压早期肾损害患者,随机分为治疗组和对照组.两组均给予常规降压治疗.治疗组同时加服养肝益水颗粒.两组疗程均为4周.分别于治疗前和治疗4周后进行UAER、MA、α1-MG、β2-MG、TRF、IgGu测定.结果:治疗后两组UAER、MA、α1-MG、β2MG、TRF、IgGu水平均显著下降,且治疗组与对照纽比较,上述指标的差异具有显著性(P<0.05,P<0.01).结论:养肝益水颗粒具有减少高血压早期肾损害尿微量蛋白排出的作用. 相似文献
43.
随着测谎技术在司法工作领域内的广泛开展 ,解决了许多以往用经验、技术无法面对和解决的问题。从以往只由物的鉴识进而发展到对人的鉴识 ,避免了由于复杂的犯罪假象而带来的侦查活动的盲目性。作为一种侦查手段的测试结论 ,为测后审讯及审讯方案提供了事实上的依据。测试活动和测试结论的得出 ,改变了审讯活动中 ,敌我双方的力量和态势 ,是促成犯罪嫌疑人作出真实供述的重要基础。犯罪心理测试作为一种技术手段用于侦查阶段 ,是行之有效的。但它不能代替侦查、审讯 ,其结论最终是通过审讯查证所获取的证据来印证、核实。 相似文献
44.
“教授法”是我国占主流的法学教育模式,该模式存在着忽视法学的实践性的缺点;统一司法考试中的刑法真题在总体上体现了素质教育理念;以司法考试为导向的法学教育不会成为新的应试教育;在统一的司法考试制度下,刑事案例演习这种新教育模式的探索具有时代意义。 相似文献
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目的:对珍灵口服液进行主要药效及毒性研究。方法:采用酶联免疫吸附(ELISA)测定小鼠血清中IgG含量,采用植物血凝素(PHA)刺激淋巴细胞转化--小鼠体内诱导法测定淋巴细胞转化率;用TBA反应比色法测定小鼠血清中脂质过氧化物(LPO)的含量,用NBT法(四氮唑蓝法)测定超氧化物歧化酶(SOD)的含量;并进行了急性和长期毒性试验。结果:小鼠血清IgG含量、淋巴细胞数、红细胞内SOD含量明显升高(P<0.05或P<0.01);血清中LPO含量明显降低(P<0.05);未见毒性反应。结论:珍灵口服液能增强机体免疫力,并具有降低机体损伤的功能,是安全有效的保健药品。 相似文献
47.
The Accuracy of Handheld Pre‐Arrest Breath Test Instruments as a Predictor of the Evidential Breath Alcohol Test Results 下载免费PDF全文
Nayak L. Polissar Ph.D. Wassana Suwanvijit Ph.D. Rod G. Gullberg M.S. PStat 《Journal of forensic sciences》2015,60(2):482-487
Drunk driving is a serious threat to public safety. All available and appropriate tools for curbing this threat should be employed to their full extent. The handheld pre‐arrest breath test instrument (PBT) is one tool for identifying the alcohol‐impaired driver and enforcing drunk driving legislation. A set of data was evaluated (n = 1779) where the PBT instrument was employed in drunk driving arrests to develop a multivariate predictive model. When maintained and operated by trained personnel, the PBT provides a reasonable estimate of the evidential test result within the relevant forensic range (95% prediction interval: ± 0.003 g/210 L). ROC analysis shows that a multivariate model for PBT prediction of the evidentiary alcohol concentration above versus below the legal limit of 0.08 g/210 L has excellent performance with an AUC of 0.96. These results would be of value in evidential hearings seeking to admit the PBT results in drunk driving trials. 相似文献
48.
Huipin Wang M.D. Yan Liu M.D. Jian Zhao B.D. Sunlin Hu Ph.D. Yuzhong Wang M.D. Chao Liu Ph.D. Yanji Zhang B.D. 《Journal of forensic sciences》2015,60(Z1):S227-S230
Presence of diatoms in tissues has been considered as a significant sign of drowning. However, there are limitations in the present extraction methods. We developed a new digestion method using the Lefort aqua regia solution (3:1 nitric acid to hydrochloric acid) for diatom extraction and evaluated the digestive capability, diatom destruction, and diatoms' recovery of this new method. The kidney tissues from rabbit mixed with water rich in diatoms were treated by the Lefort aqua regia digestion method (n = 10) and the conventional acid digestion method (n = 10). The results showed that the digestive capability of Lefort aqua regia digestion method was superior to conventional acid digestion method (p < 0.01); the structure of diatom remained almost intact; and the recovery of diatom was comparable to the conventional acid digestion method (p > 0.05). The Lefort aqua regia reagent is an improvement over the conventional acid digestion for recovery of diatoms from tissue samples. 相似文献
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50.
利用边缘无形体可溶性抗原 ,以化学交联的方式致敏羧化胶乳制成胶乳抗原 ,成功地建立了一种检测牛边缘无形体血清抗体的胶乳凝集试验 (LAT)诊断方法。用制备的胶乳抗原分别检测瑟氏泰勒虫、双芽巴贝虫、衣原体、附红细胞体阳性血清 ,结果均为阴性 ;与边缘无形体标准阳性血清、免疫牛血清、流行区血清样品均呈明显的凝集反应。研究结果表明 ,LAT方法具有操作简单、快速、敏感性高、特异性强、成本低廉等优点 ,是一种特别适合于基层现场检测边缘无形体血清抗体的新方法。 相似文献