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21.
崔晓玲 《山西青年管理干部学院学报》2012,25(1):27-28,32
企业团组织引导青年发展,应基于青年特点和企业的实际展开。企业团组织要发挥政治上引领青年、帮助青年成才、对青年的考核等方面的作用。企业团建工作要从健全组织机构、介入企业文化、推进组织创新、规范组织生活、开展青年养成教育等方面入手。 相似文献
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DNA侦查技术克服了以往遗传标记检测的种种缺陷以及指纹技术的一些局限 ,实现了物证检验从否定到认定的飞跃。目前DNA侦查技术正在走向更广阔、更深入的领域 ,出现了具有良好应用前景的一些新技术 ;许多国家均投资建立国家DNA犯罪数据库。我国的DNA犯罪数据库也在加紧建设中。从目前我国的有关情况看 ,建立DNA技术标准化和质量控制体系 ,已成为我国法庭科学亟待解决的问题。然而 ,DNA鉴定的不断完善 ,还有赖于人类基因组计划。 相似文献
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Hari Jang M.S. Sang Eon Shin Ph.D. Kyu Jin Youm B.S. Mustafa Zafer Karagozlu Ph.D. Chang Bae Kim Ph.D. Kwang Soo Ko M.S. Seong Hwan Park M.D. Ph.D. 《Journal of forensic sciences》2020,65(1):283-287
Species identification of necrophagous insects found on a dead body is an essential key in applying medicolegal entomology to the estimation of postmortem interval (PMI). Due to limited morphological identification of insect evidence, several studies have identified species using molecular information such as DNA markers. While considerable cytochrome c oxidase subunit I (COI) gene sequence data of necrophagous fly species have been collected and annotated, those of necrophagous beetle species have not. Since necrophagous beetles such as Dermestes species have a larval period longer than that of flies, beetles are useful in even the late decomposition phase in estimating minimum PMI. To obtain the full-length COI gene sequences of six Dermestes species collected from South Korea, we designed primers for polymerase chain reaction amplification and sequencing. The obtained full COI nucleotide sequences were used for performing phylogenic analysis and comparison with previously reported sequences. The results demonstrated that the COI gene sequences could be used to identify forensically important Dermestes species in South Korea. 相似文献
25.
Ju Yeon Jung M.S. Ga Hee Ryu B.S. Da-Hye Kim B.S. Joo-Young Kim Ph.D. In Kwan Hwang Ph.D. Pil-Won Kang Ph.D. Byung Won Chun Ph.D. Seong Yeon Yoo Ph.D. 《Journal of forensic sciences》2020,65(1):209-213
When using non-FTA cards in commercial multiplex STR kits for direct PCR, pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non-FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep-n-Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set-up to analyze reference samples collected using a non-FTA card manufactured in Korea. 相似文献
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Charlotte Murphy Ph.D. June Kenna M.Sc. Lorna Flanagan Ph.D. Marce Lee Gorman B.Sc. Clara Boland Ph.D. Jennifer Ryan Ph.D. 《Journal of forensic sciences》2020,65(2):399-405
This study is the first to examine the background level of male DNA on underpants worn by females in the absence of sexual contact. Here, we examined 103 samples from the inside front of underpants from 85 female volunteers. Samples were examined for the presence of male DNA using NGM SElect and PowerPlex Y23 kits. Only five samples gave a “complete” Y-STR profile, even though 83.5% of our volunteers cohabited with a male. In all cases where a partner reference sample was available, the Y-STR profile matched the cohabiting partner. We have demonstrated that a Y-STR profile is not expected on the inside front of underpants worn by females after social contact alone. The results of this study are informative for evaluating the significance of a Y-STR profile on underpants in cases of alleged sexual assault. 相似文献
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Sabrina Zorbo M.S. Patrick P. J. M. H. Jeuniaux Ph.D. 《Journal of forensic sciences》2020,65(2):641-648
In the case of suspicious deaths, the technique of 1:1 taping is often used in Belgium. It consists of affixing a large number of adhesive tapes to the body of the victim. It is conventionally aimed at obtaining microtraces (e.g., fibers, hair) and is usually not used for DNA analysis. However, in some cases, DNA analysis of certain areas of interest identified on the 1:1 taping material can offer a last resort solution. The four-step method that is described in this article involves the selection of areas of interest on the body (Step 1), the selection of the corresponding tapes (Step 2), the decontamination of the tapes (Step 3), the selection of areas of interest on the tapes, for DNA sampling (Step 4). The method is illustrated by its successful application in four murder cases. In each case, DNA profiles of good quality could be identified, including profiles of persons different from the victim. 相似文献
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Min Li Ruiyang Tao Wei Zhou Yanan Li Meng Meng Yilun Zhang Linsheng Yu Liqin Chen Yingnan Bian Chengtao Li 《法庭科学研究(英文)》2021,6(1):84
Short tandem repeat (STR) profiling is one of the mostly used systems for forensic applications. In certain circumstances, STR profiling is time-consuming and costly, which potentially leads to delays in criminal investigations. LGC (Laboratory of the Government Chemist, UK) Forensics has developed a robust STR profiling platform called the ParaDNA® Intelligence Test System which can provide early tactical intelligence and aid investigators in making informed decisions on sample prioritization for detection. Here, we validated the ParaDNA intelligence test for its application in forensic cases using a range of mock evidence items following guidelines set by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Specifically, we tested the sensitivity and accuracy of the ParaDNA intelligence test, as well as the success rates for detecting mock samples and for use in case scenarios. Our findings demonstrate that the ParaDNA intelligence test generates useful DNA profiles, especially for samples such as blood, saliva, and semen that contain ample DNA, indicating the benefits of including ParaDNA as a prior step in forensic STR profiling pipelines. 相似文献
30.
王旭东 《吉林公安高等专科学校学报》2011,(1):55-57
寻找和研究SNP已成为后基因组时代研究的重要内容和目标。Y染色体的拟常染色体非重组区段的SNP标记,是目前公认的研究早期人类进化和迁移以及法医学应用最理想的工具。Y染色体单核苷酸多态性(SNP)分型和检测方法的发展,决定了它能被广泛运用于刑事侦查当中。Y染色体的命名、标记和分类等技术分析方法有待进一步地发展,以提高其识别的规范性与稳定性。 相似文献