荧光标记复合扩增检测4个STR基因座多态性

目的建立荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座检测分型方法,并对成都汉族群体4个基因座的遗传多态性进行调查。方法用6-FAM标记D1S2142和D15S659引物,HEX、TMR分别标记D14S306和D13S1492引物,PCR复合扩增,310基因分析仪电泳自动收集电泳结果数据,GeneScan Analysis Software3.7NT软件计算扩增产物片段相对大小,Genotyper(3.7NT软件进行样本基因型分型,建立了荧光标记复合扩增检测4个STR基因座基因型的方法,对145名成都汉族无关个体样本进行分型。结果荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座,每个STR基因座都获得了清晰的基因型分型结果。145份样本,4个STR基因座分别检出10,14,7,12个等位基因和22,54,21,39种基因型,其基因型分布均符合Hardy-W e inberg平衡。4个基因座在成都汉族群体的杂合度分别依次为0.7793,0.8345,0.7793和0.8345;多态信息含量分别依次为:0.7656,0.8730,0.7470和0.8312。累计非父排除率为0.9783,累计个人识别机率为0.9999 917。结论荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座,可实现对每个基因座准确分型;成都汉族群体该4个基因座的遗传学数据,可为群体遗传学和法医学研究与应用提供基础资料。     

在初中开展青春期教育的实践与反思——以“重要性回答”、“性角色定位”两课为例

新课标推行后,思想品德课程标准与原思想政治课课程标准在教学内容的比较上,增补了对初中学生进行青春期教育的内容。本章总结中学生青春期教育过程中存在的问题,结合思想品德课程教学积极探索解决问题的办法。     

论相对独立的量刑程序

量刑程序的正当性与量刑程序与定罪程序分离的可行性属于两个不同层面的问题。现阶段我国尚不具备将定罪与量刑程序完全分离的制度条件，而只能构建相对独立的量刑程序，即在法庭审理的主要环节，将定罪与量刑活动相对分离。在将量刑活动纳入法庭审理程序时，应当注意处理好量刑建议与量刑裁量权之间的关系。     

中日青少年交流回顾与思量

新中国成立后中日青少年交流从无到有、由浅入深、由点及面全面铺开，经历了起步、兴盛和平稳发展3个大的阶段。中日青少年交流主要有交流范围和领域不断扩大，交流密切度与政治关联极大，交流丰富多彩、形式多样，着眼长远，走向机制化以及矛盾与发展相辅相成5个显而易见的特点。两国青少年交流对两国政治、经济、文化等各个方面都有很大影响，最突出的表现为有利于拉近两国国民距离，增进国民感情；有利于减少两国民间误解，增强政治互信。展望未来两国青少年交流，两国应该采取着力建立中日青少年交流长效机制、进行正确的历史观教育、加大两国留学生交流等对策。     

用超薄层聚丙烯酰胺凝胶等电聚焦法调查西藏藏族群体GC亚型频率报道

作者采用超薄层聚丙烯酰胺凝胶等电聚焦法检测了142例西藏地区无关藏族青年男女GC亚型,发现6例变异型,其中2例1A变异;1例1c变异;3例1F1S-2变异,这种新的变异型,迄今国内外尚未见报道。GC亚型的基因频率分别为:GC*1F=0.3921、GC*1S=0.3705、GC*2=0.2266、GC*1A=0.0072、GC*1C=0.0036。个人识别机率为0.8079;非父排除率为0.3430。     

Community-based forensic child and adolescent mental health services in England,Scotland and Wales: a national mapping exercise

Aim: We sought to ascertain the current nature of specialist community mental health provision for young people presenting with risk of harm to others or involvement with the youth justice system in England, Wales and Scotland. Methods: Questionnaire-based survey. Results: Fifty-five services were contacted, 48 responded and 35 of these were found to be providing some form of community mental health provision to high-risk young people. Thirteen services provided comprehensive community forensic child and adolescent mental health (FCAMH) services, whilst the remainder offered more ad hoc clinical input. Amongst the comprehensive FCAMH services, staffing, function, commissioning arrangements and population served varied widely. Conclusions: There is patchy geographical provision of comprehensive community FCAMH services across England, Scotland and Wales with heterogeneous commissioning arrangements, staffing and function. Development of a national commissioning strategy for community FCAMH services is required to ensure that the complex needs of high-risk young people are adequately addressed.     

国际人权公约与中国宪法

由于历史背景、立法精神和逻辑起点等不同,《经济、社会和文化权利国际公约》和《公民权利和政治权利国际公约》,这两个国际人权公约与我国宪法存在差异。我国人权宪政机制的整合需要完善国内宪法,发挥解释机制的作用;签署或批准公约时声明保留;正确处理我国宪法与两个国际人权公约的效力问题;人权原则的制度化和法律化等。     

主观主义与客观主义之分野及趋同

客观主义从人的共同理性、人格同一性出发 ,以行为为中心 ,注重行为客观社会危害的犯罪评价 ;主观主义从人的超越理性、人格特殊性出发 ,以行为人为中心 ,强调行为人的人身危险性的犯罪评价。 1 9世纪以来 ,主观主义、客观主义由对立走向折衷、调和以至统一 ,形成了折衷主义。刑法典与司法运作折射着其背后的理论蕴含。在当代刑法中 ,量刑不仅要依据行为、实害、故意、过失 ,而且需考虑行为人的人身危险性 ,此为量刑之折衷 ;人身危险性从两个角度介入定罪 ,是为定罪之折衷。     

Peer pressure and adolescent substance use

Peer influence is regarded as one of the strongest determinants of juvenile delinquency and particularly adolescent substance use. A commonly held view is that social pressure from friends to use drugs and alcohol is a major contributor to substance use. Yet the notion of peer pressure, implied by the association between peer-group associations and drug behavior, is seldom tested empirically. As a crucial test of the group pressure model, this research examines the role of peer pressure in mediating the effect of differential association on individual use. Moreover, few studies examine the nature of the relationship between peers and substance use as it relates to the processes leading toand from use. Drawing on differential association and social learning theories, our research specifies the social processes (socialization, group pressure, social selection, and rationalization) which dictate particular causal pathways leading to and from substance use and then estimates the reciprocal influences among differential association, social pressure from peers, attitudes favorable toward substance use, and individual use. Using the 1977–1979 National Youth Survey panel data, we estimate a covariance structural equation model allowing for correlated measurement error. In the cross-sectional analyses, we find no main effects of overt peer pressure on substance use. Estimation of the reciprocal effects model also reveals that overt peer pressure does not significantly influence substance use and does not mediate the effect of differential association. Instead, the influences of socialization, social selection, and rationalization play significant roles in understanding substance use.An earlier version of this paper was presented at the 1994 American Society of Criminology meetings in Miami, Florida.