嗜水气单胞菌gyrA氟喹诺酮抗性决定区的克隆与分析

研究了从病鱼体内分离的嗜水气单胞菌对氟喹诺酮类药物耐药性的分子机理。以 4株对诺氟沙星等氟喹诺酮类药物耐药菌株及 2株敏感菌株为模板 ,参照杀鲑气单胞菌gyrA基因序列 ,设计了 1对引物 ,进行 gyrA基因氟喹诺酮抗性决定区PCR扩增 ,将扩增产物克隆入pMD1 8 T载体 ,转化入大肠埃希氏菌DH5α中 ,小提质粒 ,酶切鉴定 ,测序并分析比较耐药菌和敏感菌的氨基酸残基序列 ,发现耐药菌有 5个氨基酸突变位点 ,分别是 83位点的Ser→Ile,92位点的Leu→Met,1 74位点的Ile→Phe,2 0 2位点的Asn→Asp ,2 0 3位点的Leu→Arg ,耐药菌突变后的氨基酸残基均比敏感菌正常的相对应氨基酸残基分子量大。 83位点的突变与大肠埃希氏菌的耐药性突变一致 ,1 2 2位点具有保守的Try。     

论行政相对人的抵抗权利

行政相对人的抵抗权利,即行政抵抗权,是行政相对人个体对行政行为所设置的义务进行抵制和不服从的权利。本文首先对行政抵抗权进行了界定,其次又对我国行政抵抗权的立法现状进行了评论,并在此基础上提出了自己的完善建议。     

浅析中原战役国民党军队大溃败之原因

中原战役是抗日战争时期豫湘桂战役的首战,全面探析国民党军队在这次战役中溃败的原因,将有助于了解认识造成国民党军队失败的深层原因。     

Labour Discipline and Resistance: The Oruro Mining District in the Late Colonial Period

This article discusses the characteristics of the (principally indigenous) work force in the mines of Charcas, Bolivia, in the last decades of the eighteenth century. It analyses the ways in which mine workers were disciplined under law and in daily practice, as well as their resistance to such discipline, so as to assess the application and impact of mining legislation. The chief obstacles to proletarianization lay in the characteristic mixture in the mines of wage labourers and peasants, as well as in the different concepts of work held by Spaniards and Indians. An increase in coercion during this period, both by the State and private individuals, so as to recruit and retain workers provoked different strategies of resistance among the Indians, as is illustrated by a significant joint protest among smelting–mill workers in Oruro and Paria in 1793.     

Disentangling the Effects of Self-Protective Behaviors on the Risk of Injury in Assaults Against Women

Using data from the National Crime Victimization Survey, this paper attempts to disentangle the effects of self-protective behaviors on the risk of injury in assaults against women. Unlike previous research, in this study we address simultaneously three important conceptual and methodological issues: (1) type of self-protective behavior, (2) temporal sequencing of self-protective behavior in relation to injury, and (3) the victim/offender relationship. Results indicate that even after controlling for other contextual characteristics of an assault, the probability of a woman being injured was lowest when she employed non-physical resistance strategies such as arguing or reasoning with the offender. This was true for all types of offenders. However, for assaults involving intimates, the probability of injury was increased for women who physically resisted their attackers.     

垃圾卫生填埋场复合密封层防渗技术及应用

介绍了垃圾卫生填埋场防渗系统中密封材料HDPE膜及GCL的主要性能及特点。提出在我国应用新的填埋场密封材料GCL ,与HDPE膜组成复合密封层 ,并从防渗性能、价格等方面进行了比较分析 ,同时提出HDPE膜—GCL复合密封层建设规范的建议     

Reading Václav Havel in the Age of Trump

This paper explores the explicit self-deprecation in Vaclav Havel’s writings in order to outline a unique contribution to thinking about resistance contained in Havel’s oeuvre. What Havel offers is a timely critique of messianic political gestures. I will focus on how Havel’s self-deprecation offers a subtle but significant critique of western politics which also contains the kernal of a virtue ethics of sorts, instead of a blueprint for radical action. I will also explain the important and related critique of messianic politics entailed in Havel’s thought and outline the significance of this critique for the current political climate.     

Just war and the Lebanese resistance to Israel

The literature on Lebanese resistance to Israel is overwhelmed with work on Hezbollah, the role of religion, and its connection to Iranian influence. However, few of these studies have looked at the totality of Lebanese resistance, from its secular origins to its Islamic monopoly. Moreover, no work to date has looked at Lebanese resistance through the prism of just war theory. This article aims at addressing this gap by applying the criteria introduced by Childress regarding the justness of war. Moreover, the article examines resistance as a practice of non-state actors and its terrorist label, and at the same time, evaluates Israel’s military response to Lebanese resistance through the prism of state terrorism.     

New Ways of Making and Living Politics: The Movimiento de Trabajadores Desocupados de Solano and the 'Movement of Movements'

This article develops a critique of liberal and social democratic conceptualisations of the political that seek to analyse contemporary social movements in Latin America, arguing that they often silence, misinterpret and/or de-legitimise new ways of living and making politics in the region. It develops an alternative conceptualisation, based upon the insights of post-structuralism and heterodox Marxism, which offers the analytic means to engage more fully with territorialised movements that politicise place, subjectivity and everyday social relations. It illustrates the potential use of such a framework with reference to the Movimiento de Trabajadores Desocupados de Solano of Greater Buenos Aires.     

论不动产物权登记的效力

登记的对抗力是一把双刃剑,以登记与否来确定权利的归属;而公信力则保护信赖登记的善意第三人取得不实登记的权利,以牺牲真正权利人的权利。登记的推定力是为了克服所有权取得过程中权原证明上的困难而采取的一种法律上的对策,斩断了登记名义人以前的一切权利关系,从而为物权交易创设新的权原起点。